Construct: sgRNA BRDN0001147357
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCAATGTAATGAACCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ILK (3611)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75909
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
6608150 |
+ |
ILK |
NNNCAATGTAATGAACCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
6608150 |
+ |
TAF10 |
NNNCAATGTAATGAACCGTG |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000002.12 |
2 |
29608573 |
+ |
ALK |
NNNCACTGTAATGAACCGTG |
NGG |
1 |
0.4545 |
Tier II |
| 4 |
NC_000012.12 |
12 |
56088919 |
- |
ERBB3 |
NNNCAATGAAATGAACTGTG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000007.14 |
7 |
147579136 |
+ |
CNTNAP2 |
NNNAAATGTAATGAACTGTG |
NGG |
2 |
0.393 |
Tier II |
| 6 |
NC_000003.12 |
3 |
44888827 |
+ |
TGM4 |
NNNCATTGTAATGAACAGTG |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000018.10 |
18 |
5195348 |
- |
AKAIN1 |
NNNCACTGTAATGAACAGTG |
NGG |
2 |
0.2121 |
Tier II |
| 8 |
NC_000010.11 |
10 |
114706640 |
+ |
ABLIM1 |
NNNCAATGTAATGAAGCCTG |
NGG |
2 |
0.0733 |
Tier II |
| 9 |
NC_000006.12 |
6 |
149459365 |
+ |
ZC3H12D |
NNNCAATGTAATGAAAGGTG |
NGG |
2 |
0.0588 |
Tier II |
| 10 |
NC_000011.10 |
11 |
64111817 |
+ |
FLRT1 |
NNNCAATGTAATGAAAGGTG |
NGG |
2 |
0.0588 |
Tier II |
| 11 |
NC_000011.10 |
11 |
64111817 |
+ |
MACROD1 |
NNNCAATGTAATGAAAGGTG |
NGG |
2 |
0.0588 |
Tier II |
| 12 |
NC_000010.11 |
10 |
79011272 |
- |
ZMIZ1-AS1 |
NNNCAATGTTGTGAACCGTG |
NGG |
2 |
0.5735 |
Tier III |
| 13 |
NC_000004.12 |
4 |
96800365 |
+ |
LINC02267 |
NNNCAATGTAATTAACAGTG |
NGG |
2 |
0.14 |
Tier III |
| 14 |
NC_000009.12 |
9 |
32724079 |
- |
LOC105376017 |
NNNCAATGTAATGAACTGTG |
NGA |
2 |
0.0324 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
142037022 |
+ |
Tmem51 |
NNNCAATGAAATGAACCGTA |
NGG |
2 |
0.8705 |
Tier II |
| 2 |
NC_000082.6 |
16 |
90081134 |
- |
Tiam1 |
NNNCAAAGTAATGAACCATG |
NGG |
2 |
0.6058 |
Tier II |
| 3 |
NC_000078.6 |
12 |
105038063 |
- |
Glrx5 |
NNNCACTGTAATGAACCATG |
NGG |
2 |
0.3147 |
Tier II |
| 4 |
NC_000081.6 |
15 |
47783561 |
+ |
Csmd3 |
NNNCAAAGTAATGAACCGTG |
NGT |
2 |
0.0141 |
Tier II |
| 5 |
NC_000078.6 |
12 |
105038063 |
- |
Gm46374 |
NNNCACTGTAATGAACCATG |
NGG |
2 |
0.3147 |
Tier III |
| 6 |
NC_000082.6 |
16 |
73263681 |
- |
4930500H12Rik |
NNNCAATGGAATGAACAGTG |
NGG |
2 |
0.2889 |
Tier III |
| 7 |
NC_000082.6 |
16 |
48402936 |
+ |
Gm5485 |
NNNCAATGTAATGAATGGTG |
NGG |
2 |
0.0181 |
Tier III |
Other clones with same target sequence:
(none)
