Construct: sgRNA BRDN0001147358

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACACCCCAGTCTACCGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROS1 (6098)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75993

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 117365634 + ROS1 NNNACCCCAGTCTACCGCAG NGG 0 1.0 Tier I
2 NC_000015.10 15 99240040 + TTC23 NNNACCACAGTCTACAGCAG NGG 2 0.75 Tier II
3 NC_000017.11 17 47698515 - TBKBP1 NNNACCCCAGACTACAGCAG NGG 2 0.75 Tier II
4 NC_000005.10 5 178367483 - COL23A1 NNNGCCCCAGTCTACCACAG NGG 2 0.5833 Tier II
5 NC_000015.10 15 90893536 - FES NNNACCCCTGTCTACCACAG NGG 2 0.56 Tier II
6 NC_000009.12 9 121210496 + GSN NNNAACCCAGTCTACCACAG NGG 2 0.5333 Tier II
7 NC_000012.12 12 104514912 - CHST11 NNNACCCCAGTTTACCGCTG NGG 2 0.2899 Tier II
8 NC_000010.11 10 133224364 - KNDC1 NNNAGCCCAGTCTGCCGCAG NGG 2 0.28 Tier II
9 NC_000010.11 10 69303802 - HK1 NNNACCCCAGTCTCCCGCAA NGG 2 0.2009 Tier II
10 NC_000001.11 1 65835687 - PDE4B NNNACCCCAGTCTACAGCAG NGC 2 0.0222 Tier II
11 NC_000005.10 5 178367483 - LOC105377757 NNNGCCCCAGTCTACCACAG NGG 2 0.5833 Tier III
12 NC_000002.12 2 220126021 + LOC105373893 NNNACCCCAGTCAACCCCAG NGG 2 0.1629 Tier III
13 NC_000005.10 5 117368149 + LOC105379139 NNNACCCCAGTGTACCCCAG NGG 2 0.1046 Tier III
14 NC_000023.11 X 131025408 - LINC01201 NNNACCCCAGTCTACCTCAG NAG 2 0.0648 Tier III
15 NC_000012.12 12 58976397 + LRIG3-DT NNNACCCCAGTCTACCACAG NGT 2 0.0151 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 52128992 + Ros1 NNNGCCCCAGTCCACCGCAG NGG 2 0.4934 Tier I
2 NC_000069.6 3 116303232 + Cdc14a NNNATCCCAGTCTACCACAG NGG 2 0.5939 Tier II
3 NC_000071.6 5 118194212 + Rnft2 NNNACCCCTGTCTACCCCAG NGG 2 0.1412 Tier II
4 NC_000077.6 11 84465791 - Aatf NNNACCCCAGTCTACTGCAG NAG 2 0.0798 Tier II
5 NC_000075.6 9 48722445 - Zbtb16 NNNACCCCAGTCTACCCCAG NAG 2 0.061 Tier II
6 NC_000068.7 2 66090813 + Galnt3 NNNACCCCAGTTTACCGCAG NTG 2 0.021 Tier II
7 NC_000077.6 11 112891728 + Gm46301 NNNACCCCAGCCTACCACAG NGG 2 0.6222 Tier III
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Other clones with same target sequence:

(none)