Construct: sgRNA BRDN0001147358
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACACCCCAGTCTACCGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROS1 (6098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75993
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
117365634 |
+ |
ROS1 |
NNNACCCCAGTCTACCGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
99240040 |
+ |
TTC23 |
NNNACCACAGTCTACAGCAG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000017.11 |
17 |
47698515 |
- |
TBKBP1 |
NNNACCCCAGACTACAGCAG |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000005.10 |
5 |
178367483 |
- |
COL23A1 |
NNNGCCCCAGTCTACCACAG |
NGG |
2 |
0.5833 |
Tier II |
| 5 |
NC_000015.10 |
15 |
90893536 |
- |
FES |
NNNACCCCTGTCTACCACAG |
NGG |
2 |
0.56 |
Tier II |
| 6 |
NC_000009.12 |
9 |
121210496 |
+ |
GSN |
NNNAACCCAGTCTACCACAG |
NGG |
2 |
0.5333 |
Tier II |
| 7 |
NC_000012.12 |
12 |
104514912 |
- |
CHST11 |
NNNACCCCAGTTTACCGCTG |
NGG |
2 |
0.2899 |
Tier II |
| 8 |
NC_000010.11 |
10 |
133224364 |
- |
KNDC1 |
NNNAGCCCAGTCTGCCGCAG |
NGG |
2 |
0.28 |
Tier II |
| 9 |
NC_000010.11 |
10 |
69303802 |
- |
HK1 |
NNNACCCCAGTCTCCCGCAA |
NGG |
2 |
0.2009 |
Tier II |
| 10 |
NC_000001.11 |
1 |
65835687 |
- |
PDE4B |
NNNACCCCAGTCTACAGCAG |
NGC |
2 |
0.0222 |
Tier II |
| 11 |
NC_000005.10 |
5 |
178367483 |
- |
LOC105377757 |
NNNGCCCCAGTCTACCACAG |
NGG |
2 |
0.5833 |
Tier III |
| 12 |
NC_000002.12 |
2 |
220126021 |
+ |
LOC105373893 |
NNNACCCCAGTCAACCCCAG |
NGG |
2 |
0.1629 |
Tier III |
| 13 |
NC_000005.10 |
5 |
117368149 |
+ |
LOC105379139 |
NNNACCCCAGTGTACCCCAG |
NGG |
2 |
0.1046 |
Tier III |
| 14 |
NC_000023.11 |
X |
131025408 |
- |
LINC01201 |
NNNACCCCAGTCTACCTCAG |
NAG |
2 |
0.0648 |
Tier III |
| 15 |
NC_000012.12 |
12 |
58976397 |
+ |
LRIG3-DT |
NNNACCCCAGTCTACCACAG |
NGT |
2 |
0.0151 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
52128992 |
+ |
Ros1 |
NNNGCCCCAGTCCACCGCAG |
NGG |
2 |
0.4934 |
Tier I |
| 2 |
NC_000069.6 |
3 |
116303232 |
+ |
Cdc14a |
NNNATCCCAGTCTACCACAG |
NGG |
2 |
0.5939 |
Tier II |
| 3 |
NC_000071.6 |
5 |
118194212 |
+ |
Rnft2 |
NNNACCCCTGTCTACCCCAG |
NGG |
2 |
0.1412 |
Tier II |
| 4 |
NC_000077.6 |
11 |
84465791 |
- |
Aatf |
NNNACCCCAGTCTACTGCAG |
NAG |
2 |
0.0798 |
Tier II |
| 5 |
NC_000075.6 |
9 |
48722445 |
- |
Zbtb16 |
NNNACCCCAGTCTACCCCAG |
NAG |
2 |
0.061 |
Tier II |
| 6 |
NC_000068.7 |
2 |
66090813 |
+ |
Galnt3 |
NNNACCCCAGTTTACCGCAG |
NTG |
2 |
0.021 |
Tier II |
| 7 |
NC_000077.6 |
11 |
112891728 |
+ |
Gm46301 |
NNNACCCCAGCCTACCACAG |
NGG |
2 |
0.6222 |
Tier III |
Other clones with same target sequence:
(none)
