Construct: sgRNA BRDN0001147361
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTACTCACCCAACCCTCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCH (5583)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76913
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
61322457 |
- |
PRKCH |
NNNACTCACCCAACCCTCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
64726153 |
+ |
EYS |
NNNATTCAACCAACCCTCGA |
NGG |
2 |
0.5455 |
Tier II |
| 3 |
NC_000004.12 |
4 |
76108747 |
+ |
ART3 |
NNNACTTACCCAACCCTAGA |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000017.11 |
17 |
49022521 |
- |
IGF2BP1 |
NNNACTCACCCAGCCCTCCA |
NGG |
2 |
0.2924 |
Tier II |
| 5 |
NC_000013.11 |
13 |
113022996 |
- |
MCF2L |
NNNACTCTCCCAGCCCTCGA |
NGG |
2 |
0.2795 |
Tier II |
| 6 |
NC_000002.12 |
2 |
27001218 |
+ |
MAPRE3 |
NNNACTCACCCAAACCTAGA |
NGG |
2 |
0.1885 |
Tier II |
| 7 |
NC_000012.12 |
12 |
131993671 |
+ |
EP400 |
NNNACTCACCCAAACCTAGA |
NGG |
2 |
0.1885 |
Tier II |
| 8 |
NC_000014.9 |
14 |
74438503 |
+ |
SYNDIG1L |
NNNACTCACCCCACCCTCAA |
NGG |
2 |
0.188 |
Tier II |
| 9 |
NC_000011.10 |
11 |
61853856 |
+ |
FADS2 |
NNNCCTCCCCCAACCCTCGA |
NGG |
2 |
0.1513 |
Tier II |
| 10 |
NC_000014.9 |
14 |
61322457 |
- |
PRKCH-AS1 |
NNNACTCACCCAACCCTCGA |
NGG |
0 |
1.0 |
Tier III |
| 11 |
NC_000005.10 |
5 |
38077757 |
+ |
LOC105374730 |
NNNACTCACACAACCCTAGA |
NGG |
2 |
0.4667 |
Tier III |
| 12 |
NC_000005.10 |
5 |
38077757 |
+ |
LINC02107 |
NNNACTCACACAACCCTAGA |
NGG |
2 |
0.4667 |
Tier III |
| 13 |
NC_000002.12 |
2 |
27001218 |
+ |
MAPRE3-AS1 |
NNNACTCACCCAAACCTAGA |
NGG |
2 |
0.1885 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
73585518 |
- |
Prkch |
NNNACTTACCCAGCCCTCGA |
NGG |
2 |
0.5299 |
Tier I |
| 2 |
NC_000068.7 |
2 |
120311025 |
+ |
Pla2g4f |
NNNACTCACCCAACTCTCCA |
NGG |
2 |
0.0299 |
Tier I |
| 3 |
NC_000067.6 |
1 |
184728166 |
+ |
Hlx |
NNNACTCACCCAACCCACTA |
NGG |
2 |
0.3556 |
Tier II |
| 4 |
NC_000067.6 |
1 |
85245778 |
+ |
C130026I21Rik |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000067.6 |
1 |
85245778 |
+ |
Gm38510 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 6 |
NC_000067.6 |
1 |
85285353 |
- |
Gm38510 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000067.6 |
1 |
85583651 |
+ |
Sp110 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 8 |
NT_166281.1 |
1 |
22101 |
+ |
LOC677525 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 9 |
NT_166281.1 |
1 |
197464 |
- |
LOC100041057 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 10 |
NT_166282.1 |
1 |
135292 |
+ |
LOC102638047 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier II |
| 11 |
NC_000081.6 |
15 |
73006721 |
+ |
Trappc9 |
NNNACTCACCCAGCCCTGGA |
NGG |
2 |
0.087 |
Tier II |
| 12 |
NT_162750.1 |
1 |
22036 |
+ |
LOC664787 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier III |
| 13 |
NT_166281.1 |
1 |
149862 |
+ |
LOC100041034 |
NNNAGTCTCCCAACCCTCGA |
NGG |
2 |
0.2571 |
Tier III |
| 14 |
NC_000081.6 |
15 |
38446511 |
- |
G930009F23Rik |
NNNACTCACCCAAACCTCAA |
NGG |
2 |
0.25 |
Tier III |
| 15 |
NC_000079.6 |
13 |
102577930 |
- |
Gm29927 |
NNNACTCACCCAGGCCTCGA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
