Construct: sgRNA BRDN0001147362
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCGATGAGACTTCATGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG1 (5571)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77747
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
49005814 |
+ |
PRKAG1 |
NNNGCGATGAGACTTCATGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
108228477 |
+ |
NRCAM |
NNNGCGATGGGACTTCATGA |
NGG |
1 |
0.5556 |
Tier II |
| 3 |
NC_000011.10 |
11 |
133055459 |
+ |
OPCML |
NNNGTGGTGAGACTTCATGA |
NGG |
2 |
0.4492 |
Tier II |
| 4 |
NC_000012.12 |
12 |
49005814 |
+ |
DDN-AS1 |
NNNGCGATGAGACTTCATGA |
NGG |
0 |
1.0 |
Tier III |
| 5 |
NC_000010.11 |
10 |
112334361 |
+ |
GUCY2GP |
NNNGCCATGAGACTTCAGGA |
NGG |
2 |
0.2273 |
Tier III |
| 6 |
NC_000004.12 |
4 |
27274785 |
+ |
LINC02261 |
NNNGTGATGAGACTTCATGA |
NGC |
2 |
0.0141 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
98815947 |
+ |
Prkag1 |
NNNGCGATGAGACTTCATGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
66776759 |
+ |
Tg |
NNNGAGAAGAGACTTCATGA |
NGG |
2 |
0.4571 |
Tier II |
| 3 |
NC_000079.6 |
13 |
51464427 |
- |
Shc3 |
NNNGAGATGAGACTTCATGG |
NGG |
2 |
0.437 |
Tier II |
| 4 |
NC_000085.6 |
19 |
32014741 |
+ |
Asah2 |
NNNGGGATGAGGCTTCATGA |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000075.6 |
9 |
92548931 |
- |
Plod2 |
NNNGGGATTAGACTTCATGA |
NGG |
2 |
0.32 |
Tier II |
| 6 |
NC_000081.6 |
15 |
65957310 |
- |
Hhla1 |
NNNGCGATGAGACTTCACTA |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000071.6 |
5 |
136846728 |
- |
Col26a1 |
NNNGAGATGAGACTCCATGA |
NGG |
2 |
0.1558 |
Tier II |
| 8 |
NC_000074.6 |
8 |
40342275 |
- |
Micu3 |
NNNGAGATGAGACTTCATGA |
NAG |
2 |
0.1481 |
Tier II |
| 9 |
NC_000068.7 |
2 |
103446431 |
- |
Elf5 |
NNNGAGATGAGACTTCATGC |
NGG |
2 |
0.1299 |
Tier II |
Other clones with same target sequence:
(none)
