Construct: sgRNA BRDN0001147363

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAATGAGGAGGATCACACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKMT2 (1160)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76595

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 81257783 + CKMT2 NNNTGAGGAGGATCACACCA NGG 0 1.0 Tier I
2 NC_000015.10 15 43216397 - EPB42 NNNTGAGGAGCACCACACCA NGG 2 0.3383 Tier I
3 NC_000004.12 4 8302507 + HTRA3 NNNTGCGGACGATCACACCA NGG 2 0.1818 Tier I
4 NC_000004.12 4 70644295 + ENAM NNNTGAGGAGGAACACACCA NGT 2 0.0112 Tier I
5 NC_000003.12 3 142795398 + TRPC1 NNNTGAGGAAAATCACACCA NGG 2 0.9333 Tier II
6 NC_000015.10 15 66359001 + TIPIN NNNTGAGCAAGATCACACCA NGG 2 0.5744 Tier II
7 NC_000002.12 2 69945041 + ASPRV1 NNNTGAGGAGGGCCACACCA NGG 2 0.5702 Tier II
8 NC_000009.12 9 23803504 - ELAVL2 NNNTGAGGAGGAACGCACCA NGG 2 0.45 Tier II
9 NC_000002.12 2 179860137 - ZNF385B NNNTGAGGAATATCACACCA NGG 2 0.359 Tier II
10 NC_000009.12 9 123092924 + RABGAP1 NNNTCAGGAGCATCACACCA NGG 2 0.3367 Tier II
11 NC_000003.12 3 38046119 + DLEC1 NNNTGAGGAGGATTACACAA NGG 2 0.3143 Tier II
12 NC_000017.11 17 12003923 + ZNF18 NNNTGAGGAGGCCCACACCA NGG 2 0.2078 Tier II
13 NC_000004.12 4 82524983 + TMEM150C NNNTGAGGAGGATTACAGCA NGG 2 0.0978 Tier II
14 NC_000010.11 10 110788181 - RBM20 NNNAGAGGAGGATCACAGCA NGG 2 0.0635 Tier II
15 NC_000019.10 19 41673916 + CEACAM7 NNNTCAGGAGGATCACACCA NGA 2 0.0546 Tier II
16 NC_000004.12 4 2570738 - FAM193A NNNTGAGGAGGAGCTCACCA NGG 2 0.0522 Tier II
17 NC_000011.10 11 46384931 - CHRM4 NNNTGAGGAGGCTCACCCCA NGG 2 0.0464 Tier II
18 NC_000011.10 11 8958633 - TMEM9B NNNTGAGGAAGATCACACCA NTG 2 0.0364 Tier II
19 NC_000015.10 15 100884752 - ALDH1A3 NNNTGAGGAGGATCTCACCA NCG 2 0.0214 Tier II
20 NC_000018.10 18 37322745 + CELF4 NNNTGAGGAGGATCACGCCA NCG 2 0.0189 Tier II
21 NC_000017.11 17 64215322 + TEX2 NNNTGAGGAGGATGACACCA NTG 2 0.0 Tier II
22 NC_000005.10 5 81257783 + CKMT2-AS1 NNNTGAGGAGGATCACACCA NGG 0 1.0 Tier III
23 NC_000011.10 11 41714599 + LINC01499 NNNTGAAGAGGATCACATCA NGG 2 0.6429 Tier III
24 NC_000016.10 16 11290737 + LOC105371082 NNNTGTGGAGGCTCACACCA NGG 2 0.188 Tier III
25 NC_000002.12 2 52835344 - LOC105369165 NNNTGAGGAGGCTCAGACCA NGG 2 0.0405 Tier III
26 NC_000002.12 2 122309792 + LOC105373592 NNNTGAGGAGGATTACACCA NTG 2 0.0286 Tier III
27 NC_000018.10 18 51652703 - LOC105372120 NNNTGAGCAGGATCACACCA NTG 2 0.024 Tier III
28 NC_000008.11 8 143198658 - LOC107986906 NNNTGAGGAGGATCACACAA NTG 2 0.0167 Tier III
29 NC_000002.12 2 145013017 - TEX41 NNNTGAGGAGGATGACACCA NGA 2 0.0 Tier III
30 NC_000002.12 2 145013017 - LOC100505498 NNNTGAGGAGGATGACACCA NGA 2 0.0 Tier III
31 NC_000018.10 18 48901486 - LOC124904355 NNNTGAGGAGGATGACACCA NGT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 91859269 - Ckmt2 NNNTGAGGAAGACCACACCA NGG 2 0.7368 Tier I
2 NC_000068.7 2 154546687 - Necab3 NNNTGAGGAGGACCTCACCA NGG 2 0.1579 Tier I
3 NC_000072.6 6 118122109 + Csgalnact2 NNNTGAGGAGGGTCACACCA NGG 1 0.7222 Tier II
4 NC_000077.6 11 11624308 - 4930512M02Rik NNNTGAGAAGGAACACACCA NGG 2 0.6923 Tier II
5 NC_000074.6 8 80000233 + Hhip NNNTGAGGAGGAACACACCA NGG 1 0.6923 Tier II
6 NC_000070.6 4 72119254 - Tle1 NNNTGAACAGGATCACACCA NGG 2 0.6154 Tier II
7 NC_000071.6 5 37310995 - Evc NNNTGGGGAGGATTACACCA NGG 2 0.5238 Tier II
8 NC_000083.6 17 69191124 - Epb41l3 NNNAGAAGAGGATCACACCA NGG 2 0.4762 Tier II
9 NC_000076.6 10 27098319 - Lama2 NNNTGATGAGGGTCACACCA NGG 2 0.4127 Tier II
10 NC_000080.6 14 34352656 - Fam25c NNNTGAGGAGGATTACAACA NGG 2 0.3949 Tier II
11 NC_000077.6 11 100674815 - Zfp385c NNNAGAGGAGGACCACACCA NGG 2 0.3759 Tier II
12 NC_000071.6 5 36950083 - Ppp2r2c NNNTGAGGTGGATCACAACA NGG 2 0.3231 Tier II
13 NC_000080.6 14 70773272 + Dok2 NNNTGTGGAGGCTCACACCA NGG 2 0.188 Tier II
14 NC_000073.6 7 28578703 + Pak4 NNNTCAGGAGGATCACCCCA NGG 2 0.1387 Tier II
15 NC_000069.6 3 20034864 + Hps3 NNNTAAGGAGGATCAGACCA NGG 2 0.1333 Tier II
16 NC_000067.6 1 20913604 + Paqr8 NNNTGAGGAGGTTCACACGA NGG 2 0.0417 Tier II
17 NC_000067.6 1 175132726 + Rgs7 NNNTGAGGAGGAAGACACCA NGG 2 0.0 Tier II
18 NC_000071.6 5 21684188 - Napepld NNNTGAGGAGGCTGACACCA NGG 2 0.0 Tier II
19 NC_000069.6 3 85449166 + Gm31419 NNNTGTGGGGGATCACACCA NGG 2 0.4762 Tier III
20 NC_000074.6 8 13692589 + Gm46032 NNNTGTGGAGGATCACACCT NGG 2 0.4286 Tier III
21 NC_000077.6 11 100674815 - Gm38483 NNNAGAGGAGGACCACACCA NGG 2 0.3759 Tier III
22 NC_000067.6 1 44528391 + Gm39635 NNNTTAGTAGGATCACACCA NGG 2 0.1875 Tier III
23 NC_000074.6 8 10725305 - Gm30666 NNNTGAGCAGGAGCACACCA NGG 2 0.1605 Tier III
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Other clones with same target sequence:

(none)