Construct: sgRNA BRDN0001147364

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGTGACAAACCGAGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDR2 (4921)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77972

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 162761345 - DDR2 NNNTGACAAACCGAGCACTG NGG 0 1.0 Tier I
2 NC_000016.10 16 57512823 + CCDC102A NNNTGCCAAAGCGAGCACTG NGG 2 0.1136 Tier I
3 NC_000023.11 X 149498211 - IDS NNNTGACAAACAGAGCACTG NGC 2 0.0159 Tier I
4 NC_000006.12 6 36088020 + MAPK14 NNNTGACAAAACGAGCAATG NGG 2 0.4038 Tier II
5 NC_000004.12 4 9866589 + SLC2A9 NNNTGACAACCCGAGAACTG NGG 2 0.3333 Tier II
6 NC_000016.10 16 88726098 + PIEZO1 NNNTGACAGACCCAGCACTG NGG 2 0.2807 Tier II
7 NC_000017.11 17 60466124 - APPBP2 NNNAGACAAGCCGAGCACTG NGG 2 0.2646 Tier II
8 NC_000013.11 13 37720439 + TRPC4 NNNTGAGAAACCGAGCAATG NGG 2 0.2534 Tier II
9 NC_000007.14 7 47428183 + TNS3 NNNTGACCAACCCAGCACTG NGG 2 0.1805 Tier II
10 NC_000005.10 5 80654135 + DHFR NNNTGACAAACGGAGCGCTG NGG 2 0.0784 Tier II
11 NC_000008.11 8 5883241 - LOC105377795 NNNTGACAAAACAAGCACTG NGG 2 0.6923 Tier III
12 NC_000010.11 10 31132200 - LOC105376481 NNNTGACAAACAGAGCATTG NGG 2 0.4592 Tier III
13 NC_000010.11 10 31132200 - LOC105376482 NNNTGACAAACAGAGCATTG NGG 2 0.4592 Tier III
14 NC_000016.10 16 65811003 - LOC107984823 NNNTGAGAAACCGAGCACTA NGG 2 0.4412 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 169998041 + Ddr2 NNNTGACAAACCGGGCACTG NGG 1 0.4667 Tier I
2 NC_000068.7 2 26440663 - Sec16a NNNTGACAAACAGAGCACTG NGC 2 0.0159 Tier I
3 NC_000072.6 6 96932182 + Tafa4 NNNTGAGAAACCAAGCACTG NGG 2 0.4344 Tier II
4 NC_000079.6 13 94185894 - Lhfpl2 NNNTGATAAACCGTGCACTG NGG 2 0.4333 Tier II
5 NC_000080.6 14 29550783 - Cacna2d3 NNNTGACAAACCCAGCACTA NGG 2 0.3947 Tier II
6 NC_000068.7 2 125175887 - Slc12a1 NNNTGACAATCCCAGCACTG NGG 2 0.3715 Tier II
7 NC_000077.6 11 53162380 - Fstl4 NNNTGACAACCCAAGCACTG NGG 2 0.3077 Tier II
8 NC_000067.6 1 100113306 + Cntnap5b NNNTGGCAAACCCAGCACTG NGG 2 0.3008 Tier II
9 NC_000083.6 17 46776223 - Rpl7l1 NNNTGCCAAACCCAGCACTG NGG 2 0.1914 Tier II
10 NC_000069.6 3 153566059 + St6galnac3 NNNTGACAAATTGAGCACTG NGG 2 0.1657 Tier II
11 NC_000077.6 11 61334762 - Slc47a2 NNNTGCCAAAGCGAGCACTG NGG 2 0.1136 Tier II
12 NC_000077.6 11 47186613 - Sgcd NNNTGACAAACTGAGCACTG NGA 2 0.0374 Tier II
13 NC_000067.6 1 156351088 + Axdnd1 NNNTGGCAAACCGAGCACTG NGT 2 0.0115 Tier II
14 NC_000079.6 13 94185894 - LOC115488151 NNNTGATAAACCGTGCACTG NGG 2 0.4333 Tier III
15 NC_000076.6 10 25036539 - Gm47715 NNNTGACAATCCCAGCACTG NGG 2 0.3715 Tier III
16 NC_000083.6 17 45828787 - Gm35692 NNNTGACCAACCCAGCACTG NGG 2 0.1805 Tier III
17 NC_000077.6 11 61334762 - Gm25929 NNNTGCCAAAGCGAGCACTG NGG 2 0.1136 Tier III
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Other clones with same target sequence:

(none)