Construct: sgRNA BRDN0001147364
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGTGACAAACCGAGCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DDR2 (4921)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77972
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000001.11 |
1 |
162761345 |
- |
DDR2 |
NNNTGACAAACCGAGCACTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000016.10 |
16 |
57512823 |
+ |
CCDC102A |
NNNTGCCAAAGCGAGCACTG |
NGG |
2 |
0.1136 |
Tier I |
3 |
NC_000023.11 |
X |
149498211 |
- |
IDS |
NNNTGACAAACAGAGCACTG |
NGC |
2 |
0.0159 |
Tier I |
4 |
NC_000006.12 |
6 |
36088020 |
+ |
MAPK14 |
NNNTGACAAAACGAGCAATG |
NGG |
2 |
0.4038 |
Tier II |
5 |
NC_000004.12 |
4 |
9866589 |
+ |
SLC2A9 |
NNNTGACAACCCGAGAACTG |
NGG |
2 |
0.3333 |
Tier II |
6 |
NC_000016.10 |
16 |
88726098 |
+ |
PIEZO1 |
NNNTGACAGACCCAGCACTG |
NGG |
2 |
0.2807 |
Tier II |
7 |
NC_000017.11 |
17 |
60466124 |
- |
APPBP2 |
NNNAGACAAGCCGAGCACTG |
NGG |
2 |
0.2646 |
Tier II |
8 |
NC_000013.11 |
13 |
37720439 |
+ |
TRPC4 |
NNNTGAGAAACCGAGCAATG |
NGG |
2 |
0.2534 |
Tier II |
9 |
NC_000007.14 |
7 |
47428183 |
+ |
TNS3 |
NNNTGACCAACCCAGCACTG |
NGG |
2 |
0.1805 |
Tier II |
10 |
NC_000005.10 |
5 |
80654135 |
+ |
DHFR |
NNNTGACAAACGGAGCGCTG |
NGG |
2 |
0.0784 |
Tier II |
11 |
NC_000008.11 |
8 |
5883241 |
- |
LOC105377795 |
NNNTGACAAAACAAGCACTG |
NGG |
2 |
0.6923 |
Tier III |
12 |
NC_000010.11 |
10 |
31132200 |
- |
LOC105376481 |
NNNTGACAAACAGAGCATTG |
NGG |
2 |
0.4592 |
Tier III |
13 |
NC_000010.11 |
10 |
31132200 |
- |
LOC105376482 |
NNNTGACAAACAGAGCATTG |
NGG |
2 |
0.4592 |
Tier III |
14 |
NC_000016.10 |
16 |
65811003 |
- |
LOC107984823 |
NNNTGAGAAACCGAGCACTA |
NGG |
2 |
0.4412 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000067.6 |
1 |
169998041 |
+ |
Ddr2 |
NNNTGACAAACCGGGCACTG |
NGG |
1 |
0.4667 |
Tier I |
2 |
NC_000068.7 |
2 |
26440663 |
- |
Sec16a |
NNNTGACAAACAGAGCACTG |
NGC |
2 |
0.0159 |
Tier I |
3 |
NC_000072.6 |
6 |
96932182 |
+ |
Tafa4 |
NNNTGAGAAACCAAGCACTG |
NGG |
2 |
0.4344 |
Tier II |
4 |
NC_000079.6 |
13 |
94185894 |
- |
Lhfpl2 |
NNNTGATAAACCGTGCACTG |
NGG |
2 |
0.4333 |
Tier II |
5 |
NC_000080.6 |
14 |
29550783 |
- |
Cacna2d3 |
NNNTGACAAACCCAGCACTA |
NGG |
2 |
0.3947 |
Tier II |
6 |
NC_000068.7 |
2 |
125175887 |
- |
Slc12a1 |
NNNTGACAATCCCAGCACTG |
NGG |
2 |
0.3715 |
Tier II |
7 |
NC_000077.6 |
11 |
53162380 |
- |
Fstl4 |
NNNTGACAACCCAAGCACTG |
NGG |
2 |
0.3077 |
Tier II |
8 |
NC_000067.6 |
1 |
100113306 |
+ |
Cntnap5b |
NNNTGGCAAACCCAGCACTG |
NGG |
2 |
0.3008 |
Tier II |
9 |
NC_000083.6 |
17 |
46776223 |
- |
Rpl7l1 |
NNNTGCCAAACCCAGCACTG |
NGG |
2 |
0.1914 |
Tier II |
10 |
NC_000069.6 |
3 |
153566059 |
+ |
St6galnac3 |
NNNTGACAAATTGAGCACTG |
NGG |
2 |
0.1657 |
Tier II |
11 |
NC_000077.6 |
11 |
61334762 |
- |
Slc47a2 |
NNNTGCCAAAGCGAGCACTG |
NGG |
2 |
0.1136 |
Tier II |
12 |
NC_000077.6 |
11 |
47186613 |
- |
Sgcd |
NNNTGACAAACTGAGCACTG |
NGA |
2 |
0.0374 |
Tier II |
13 |
NC_000067.6 |
1 |
156351088 |
+ |
Axdnd1 |
NNNTGGCAAACCGAGCACTG |
NGT |
2 |
0.0115 |
Tier II |
14 |
NC_000079.6 |
13 |
94185894 |
- |
LOC115488151 |
NNNTGATAAACCGTGCACTG |
NGG |
2 |
0.4333 |
Tier III |
15 |
NC_000076.6 |
10 |
25036539 |
- |
Gm47715 |
NNNTGACAATCCCAGCACTG |
NGG |
2 |
0.3715 |
Tier III |
16 |
NC_000083.6 |
17 |
45828787 |
- |
Gm35692 |
NNNTGACCAACCCAGCACTG |
NGG |
2 |
0.1805 |
Tier III |
17 |
NC_000077.6 |
11 |
61334762 |
- |
Gm25929 |
NNNTGCCAAAGCGAGCACTG |
NGG |
2 |
0.1136 |
Tier III |
Other clones with same target sequence:
(none)