Construct: sgRNA BRDN0001147371
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGCCTACTAGAAACGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKK1 (84254)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76516
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
3885567 |
- |
CAMKK1 |
NNNGCCTACTAGAAACGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
129987876 |
- |
CYRIB |
NNNGTGTACTAGAAACGGTG |
NGG |
2 |
0.3182 |
Tier II |
| 3 |
NC_000001.11 |
1 |
230391760 |
- |
PGBD5 |
NNNGGCTACTAGAAATGGTG |
NGG |
2 |
0.1846 |
Tier II |
| 4 |
NC_000010.11 |
10 |
66168298 |
- |
CTNNA3 |
NNNGCCTACTAGAAACGGTG |
NCA |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
62919461 |
- |
Pias1 |
NNNACCTACTAAAAACGGTG |
NGG |
2 |
0.84 |
Tier I |
| 2 |
NC_000075.6 |
9 |
105488697 |
+ |
Atp2c1 |
NNNGCCTACTAGAAACACTG |
NGG |
2 |
0.4444 |
Tier II |
| 3 |
NC_000077.6 |
11 |
74924946 |
- |
Srr |
NNNTCCTATTAGAAACGGTG |
NGG |
2 |
0.3182 |
Tier II |
| 4 |
NC_000075.6 |
9 |
78206644 |
+ |
Gsta4 |
NNNGCCTACTCGGAACGGTG |
NGG |
2 |
0.2609 |
Tier II |
| 5 |
NC_000071.6 |
5 |
117683196 |
- |
Ksr2 |
NNNGCCCACTAGAAATGGTG |
NGG |
2 |
0.2115 |
Tier II |
| 6 |
NC_000079.6 |
13 |
102880382 |
+ |
Mast4 |
NNNGACTACTTGAAACGGTG |
NGG |
2 |
0.1758 |
Tier II |
| 7 |
NC_000075.6 |
9 |
71554681 |
- |
Myzap |
NNNGCCTACAAGAAAGGGTG |
NGG |
2 |
0.1319 |
Tier II |
Other clones with same target sequence:
(none)
