Construct: sgRNA BRDN0001147380
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAAAGCTTCCGCCTGATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80225
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
215234946 |
- |
KCNK2 |
NNNGAGCTTCCGCTTGATGG |
NGG |
2 |
0.4583 |
Tier I |
| 2 |
NC_000016.10 |
16 |
3758006 |
+ |
CREBBP |
NNNCAGCTTCCGCTTGATGG |
NGG |
2 |
0.2588 |
Tier I |
| 3 |
NC_000011.10 |
11 |
6623326 |
- |
DCHS1 |
NNNAAGCTTCCGGCTGCTGG |
NGG |
2 |
0.0241 |
Tier I |
| 4 |
NC_000013.11 |
13 |
52848241 |
- |
PCDH8 |
NNNAAGCTTCCGCCTGATGA |
NGC |
2 |
0.0208 |
Tier I |
| 5 |
NC_000002.12 |
2 |
45906748 |
- |
PRKCE |
NNNGAGCTGCCGCCTGATGG |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000007.14 |
7 |
41019747 |
+ |
SUGCT |
NNNCAGCTTCTGCCTGATGG |
NGG |
2 |
0.1086 |
Tier II |
| 7 |
NC_000017.11 |
17 |
6896432 |
+ |
ALOX12P2 |
NNNAAGCTTCAGCCTGATGG |
NGG |
1 |
0.75 |
Tier III |
| 8 |
NC_000009.12 |
9 |
87939178 |
- |
LOC497256 |
NNNCACCTTCCGCCTGATGG |
NGG |
2 |
0.2406 |
Tier III |
| 9 |
NC_000009.12 |
9 |
88113784 |
+ |
LOC124902203 |
NNNCACCTTCCGCCTGATGG |
NGG |
2 |
0.2406 |
Tier III |
| 10 |
NC_000017.11 |
17 |
21612975 |
+ |
LOC101930665 |
NNNAACCTTCCGCCTGCTGG |
NGG |
2 |
0.1203 |
Tier III |
| 11 |
NT_187505.1 |
|
33565 |
+ |
LOC105379562 |
NNNAATCTTCCGCCTGCTGG |
NGG |
2 |
0.1176 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
79770926 |
- |
Pcdh8 |
NNNAAGCTTCCGCCTGATGA |
NGC |
2 |
0.0208 |
Tier I |
| 2 |
NC_000075.6 |
9 |
117085690 |
+ |
Rbms3 |
NNNAAGCCTCCGCCTGAAGG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000072.6 |
6 |
40086690 |
+ |
Tmem178b |
NNNAAGCTTCAGCCTGAGGG |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000067.6 |
1 |
86864937 |
- |
Dis3l2 |
NNNAAGCTTCCTCCTGTTGG |
NGG |
2 |
0.0513 |
Tier II |
| 5 |
NC_000080.6 |
14 |
54191773 |
- |
Tcra |
NNNCAGCTTCCGCATGATGG |
NGG |
2 |
0.1235 |
Tier III |
| 6 |
NC_000080.6 |
14 |
79770926 |
- |
Gm9748 |
NNNAAGCTTCCGCCTGATGA |
NGC |
2 |
0.0208 |
Tier III |
Other clones with same target sequence:
(none)
