Construct: sgRNA BRDN0001147382
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACCCACTGTGGATAGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TIE1 (7075)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76686
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
43313288 |
+ |
TIE1 |
NNNCCCACTGTGGATAGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
137471039 |
- |
PNPLA7 |
NNNTGCACTGTGGATAGACG |
NGG |
2 |
0.48 |
Tier II |
| 3 |
NC_000005.10 |
5 |
1321920 |
- |
CLPTM1L |
NNNCCCACTATGCATAGACG |
NGG |
2 |
0.393 |
Tier II |
| 4 |
NC_000016.10 |
16 |
88808301 |
+ |
CDT1 |
NNNGCCACTGTGGACAGACG |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000002.12 |
2 |
181585099 |
+ |
CERKL |
NNNCCCACTGTGTATAGAAG |
NGG |
2 |
0.1286 |
Tier II |
| 6 |
NC_000009.12 |
9 |
114287021 |
- |
COL27A1 |
NNNCCCACTGTGGATAGAGG |
NAG |
2 |
0.0324 |
Tier II |
| 7 |
NC_000007.14 |
7 |
15361129 |
- |
AGMO |
NNNCCCACTGTGGAGAGACG |
NTG |
2 |
0.0019 |
Tier II |
| 8 |
NC_000022.11 |
22 |
47796075 |
+ |
EPIC1 |
NNNTCCACTGTGGATAGACA |
NGG |
2 |
0.75 |
Tier III |
| 9 |
NC_000008.11 |
8 |
117374001 |
+ |
LOC105375716 |
NNNCCCACTTTGGATAGAAG |
NGG |
2 |
0.3482 |
Tier III |
| 10 |
NC_000009.12 |
9 |
114287021 |
- |
LOC124902252 |
NNNCCCACTGTGGATAGAGG |
NAG |
2 |
0.0324 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
69941056 |
- |
Ybx2 |
NNNCCCACTGTTGATAGGCG |
NGG |
2 |
0.1538 |
Tier I |
| 2 |
NC_000071.6 |
5 |
130014742 |
+ |
Asl |
NNNCCCACTGTAGAGAGACG |
NGG |
2 |
0.0467 |
Tier I |
| 3 |
NC_000083.6 |
17 |
8397223 |
- |
T2 |
NNNCCCACAGTGGATAGACA |
NGG |
2 |
0.8705 |
Tier II |
| 4 |
NC_000073.6 |
7 |
58799774 |
+ |
Atp10a |
NNNCCCACTGAGGATAGACA |
NGG |
2 |
0.7031 |
Tier II |
| 5 |
NC_000069.6 |
3 |
15404386 |
- |
Sirpb1a |
NNNCCCACTGTGGACAGACA |
NGG |
2 |
0.2557 |
Tier II |
| 6 |
NC_000069.6 |
3 |
15536459 |
- |
Sirpb1b |
NNNCCCACTGTGGACAGACA |
NGG |
2 |
0.2557 |
Tier II |
| 7 |
NC_000069.6 |
3 |
15826484 |
- |
Sirpb1c |
NNNCCCACTGTGGACAGACA |
NGG |
2 |
0.2557 |
Tier II |
| 8 |
NC_000080.6 |
14 |
21277966 |
+ |
Adk |
NNNCCCACTGTGGACAGACA |
NGG |
2 |
0.2557 |
Tier II |
| 9 |
NC_000084.6 |
18 |
35217582 |
+ |
Ctnna1 |
NNNCCCACTGTGGTTAGAAG |
NGG |
2 |
0.2286 |
Tier II |
| 10 |
NC_000076.6 |
10 |
45657301 |
- |
Hace1 |
NNNCCCACTGTGGATACACT |
NGG |
2 |
0.1647 |
Tier II |
| 11 |
NC_000077.6 |
11 |
70249600 |
- |
Alox12 |
NNNCCAACTGTGGATAGAGG |
NGG |
2 |
0.1161 |
Tier II |
| 12 |
NC_000075.6 |
9 |
45473243 |
- |
Dscaml1 |
NNNCCCACTGTGGCTAGATG |
NGG |
2 |
0.0989 |
Tier II |
| 13 |
NC_000067.6 |
1 |
180009059 |
+ |
Cdc42bpa |
NNNCCCACTGTGGCTAGACC |
NGG |
2 |
0.0918 |
Tier II |
| 14 |
NC_000080.6 |
14 |
28051779 |
- |
Erc2 |
NNNCCCACTGTGGCCAGACG |
NGG |
2 |
0.0584 |
Tier II |
| 15 |
NC_000077.6 |
11 |
7122432 |
+ |
Adcy1 |
NNNCCCACTGTGGAGAGACA |
NGG |
2 |
0.0469 |
Tier II |
| 16 |
NC_000077.6 |
11 |
6119602 |
+ |
Nudcd3 |
NNNCCCACTGTGGAGAGATG |
NGG |
2 |
0.0231 |
Tier II |
| 17 |
NC_000068.7 |
2 |
180278490 |
- |
Rbbp8nl |
NNNCCCACTGTGGATAGAGG |
NTG |
2 |
0.0049 |
Tier II |
| 18 |
NC_000070.6 |
4 |
114724903 |
+ |
Gm33655 |
NNNCCCACTGAGGATAGACA |
NGG |
2 |
0.7031 |
Tier III |
| 19 |
NC_000077.6 |
11 |
29619980 |
+ |
Gm12092 |
NNNCCCACTGTGGCTAGACA |
NGG |
2 |
0.2009 |
Tier III |
| 20 |
NC_000077.6 |
11 |
70249600 |
- |
Gm40191 |
NNNCCAACTGTGGATAGAGG |
NGG |
2 |
0.1161 |
Tier III |
| 21 |
NC_000085.6 |
19 |
33312807 |
- |
Rnls |
NNNGCCACTGTGGAGAGACG |
NGG |
2 |
0.025 |
Tier III |
Other clones with same target sequence:
(none)
