Construct: sgRNA BRDN0001147386
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGATGATGTTGAGTGCACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCQ (5588)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75555
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
6479072 |
- |
PRKCQ |
NNNTGATGTTGAGTGCACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
14498498 |
+ |
COPB1 |
NNNTGAAGTTAAGTGCACGA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000002.12 |
2 |
110794352 |
- |
ACOXL |
NNNTGATGTTGGGTACACGA |
NGG |
2 |
0.6797 |
Tier II |
| 4 |
NC_000016.10 |
16 |
84302109 |
+ |
WFDC1 |
NNNTGATGCTGAGTGCACAA |
NGG |
2 |
0.6593 |
Tier II |
| 5 |
NC_000006.12 |
6 |
46485677 |
- |
RCAN2 |
NNNTGATGTGGAGTGCAAGA |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000007.14 |
7 |
28031389 |
+ |
JAZF1 |
NNNAGATGTTGAGTGGACGA |
NGG |
2 |
0.0733 |
Tier II |
| 7 |
NC_000005.10 |
5 |
2021083 |
- |
LOC105374618 |
NNNTGTTGTTAAGTGCACGA |
NGG |
2 |
0.7143 |
Tier III |
| 8 |
NC_000006.12 |
6 |
149911558 |
- |
RAET1E-AS1 |
NNNTGATGCTGAGTGCAGGA |
NGG |
2 |
0.1231 |
Tier III |
| 9 |
NC_000002.12 |
2 |
95196743 |
- |
SLC2AXP1 |
NNNTGATGTTGAGGGCAAGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
137950857 |
- |
Ece1 |
NNNTTATGTTGAGTGCACTA |
NGG |
2 |
0.2 |
Tier II |
| 2 |
NC_000074.6 |
8 |
23445329 |
+ |
Sfrp1 |
NNNTGATGTTGAGTGCACCC |
NGG |
2 |
0.1019 |
Tier II |
| 3 |
NC_000083.6 |
17 |
50139626 |
+ |
Rftn1 |
NNNTGATTTTGAGGGCACGA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
