Construct: sgRNA BRDN0001147390
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTACGAGGAAAAGTACGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK4 (65266)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75657
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
42784467 |
+ |
WNK4 |
NNNCGAGGAAAAGTACGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
132925736 |
- |
CHCHD3 |
NNNAGAGGACAAGTACGATG |
NGG |
2 |
0.2807 |
Tier II |
| 3 |
NC_000002.12 |
2 |
43302282 |
- |
THADA |
NNNTTAGGAAAAGTACGATG |
NGG |
2 |
0.24 |
Tier II |
| 4 |
NC_000017.11 |
17 |
67532666 |
+ |
PITPNC1 |
NNNCGAGGAAAAGTAACATG |
NGG |
2 |
0.2353 |
Tier II |
| 5 |
NC_000002.12 |
2 |
122327150 |
- |
LOC105373592 |
NNNCGAGGAAAAGAAAGATG |
NGG |
2 |
0.619 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
101264313 |
+ |
Wnk4 |
NNNCGAGGAAAAGTACGACG |
NGG |
1 |
0.2759 |
Tier I |
| 2 |
NC_000070.6 |
4 |
119358179 |
+ |
Ccdc30 |
NNNTGAGGAAAAGTACAATG |
NGG |
2 |
0.7467 |
Tier II |
| 3 |
NC_000071.6 |
5 |
19741196 |
- |
Magi2 |
NNNGGAGGAAAAGTAAGATG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000069.6 |
3 |
99269969 |
- |
Tbx15 |
NNNCAAGGAAAAGTACTATG |
NGG |
2 |
0.2167 |
Tier II |
| 5 |
NC_000071.6 |
5 |
19741196 |
- |
Gm52766 |
NNNGGAGGAAAAGTAAGATG |
NGG |
2 |
0.5 |
Tier III |
Other clones with same target sequence:
(none)
