Construct: sgRNA BRDN0001147400
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGGGTAGAGTCTGATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT2 (208)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77505
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40256965 |
+ |
AKT2 |
NNNGGGTAGAGTCTGATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
35715995 |
+ |
ZBTB32 |
NNNTGGAAGAGTCTGATCAG |
NGG |
2 |
0.3182 |
Tier I |
| 3 |
NC_000006.12 |
6 |
165499208 |
+ |
PDE10A |
NNNGGGAAGAGTCTGATCAG |
NGG |
1 |
0.875 |
Tier II |
| 4 |
NC_000001.11 |
1 |
2562821 |
- |
TNFRSF14 |
NNNGGGCAGTGTCTGATCAG |
NGG |
2 |
0.6066 |
Tier II |
| 5 |
NC_000021.9 |
21 |
17557783 |
+ |
CXADR |
NNNTGGTAGAATCTGATCAG |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000010.11 |
10 |
68764160 |
- |
CCAR1 |
NNNGGGTAGAGTCAGATCAC |
NGG |
2 |
0.2653 |
Tier II |
| 7 |
NC_000011.10 |
11 |
105789322 |
+ |
GRIA4 |
NNNGGGTAGAGTCTCATCAA |
NGG |
2 |
0.2557 |
Tier II |
| 8 |
NC_000002.12 |
2 |
3328576 |
- |
EIPR1 |
NNNGGGTAGAGTCTGATCCA |
NGG |
2 |
0.194 |
Tier II |
| 9 |
NC_000002.12 |
2 |
3329262 |
- |
EIPR1 |
NNNGGGTAGAGTCTGATCCA |
NGG |
2 |
0.194 |
Tier II |
| 10 |
NC_000010.11 |
10 |
88900864 |
+ |
STAMBPL1 |
NNNGGCTAGAGTCTCATCAG |
NGG |
2 |
0.186 |
Tier II |
| 11 |
NC_000011.10 |
11 |
66913912 |
+ |
PC |
NNNGTGTAGAGTCTGAACAG |
NGG |
2 |
0.16 |
Tier II |
| 12 |
NC_000008.11 |
8 |
1191725 |
- |
DLGAP2 |
NNNGGGTAAAGTGTGATCAG |
NGG |
2 |
0.0877 |
Tier II |
| 13 |
NC_000011.10 |
11 |
92265807 |
- |
FAT3 |
NNNGGGTAGAGTCTCATGAG |
NGG |
2 |
0.0364 |
Tier II |
| 14 |
NC_000002.12 |
2 |
118985394 |
- |
MARCO |
NNNGGGTAGAGTGTGGTCAG |
NGG |
2 |
0.0262 |
Tier II |
| 15 |
NC_000003.12 |
3 |
48887561 |
- |
SLC25A20 |
NNNGGGTAGAGTCTGAGCAG |
NGT |
2 |
0.0019 |
Tier II |
| 16 |
NC_000007.14 |
7 |
42592354 |
+ |
LOC105375251 |
NNNGGGTAAAGACTGATCAG |
NGG |
2 |
0.5143 |
Tier III |
| 17 |
NC_000014.9 |
14 |
106396566 |
+ |
IGH |
NNNGGGTTGATTCTGATCAG |
NGG |
2 |
0.1648 |
Tier III |
| 18 |
NC_000023.11 |
X |
8784361 |
+ |
DRAXINP1 |
NNNGGGTAGATTCTGAGCAG |
NGG |
2 |
0.0452 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
132370576 |
+ |
Auts2 |
NNNGGGAAGAGTCTGATCAT |
NGG |
2 |
0.6125 |
Tier II |
| 2 |
NC_000082.6 |
16 |
28245682 |
- |
Fgf12 |
NNNGAGTAGAGTTTGATCAG |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000084.6 |
18 |
34719753 |
- |
Gfra3 |
NNNGAGGAGAGTCTGATCAG |
NGG |
2 |
0.5098 |
Tier II |
| 4 |
NC_000068.7 |
2 |
143589331 |
+ |
Pcsk2 |
NNNGGGAAGAGGCTGATCAG |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000071.6 |
5 |
129193599 |
+ |
Adgrd1 |
NNNGGGTAAAGTCTGATCTG |
NGG |
2 |
0.3462 |
Tier II |
| 6 |
NC_000075.6 |
9 |
35789760 |
+ |
Pate6 |
NNNGGGTAGAGTCTGATGAG |
NGG |
1 |
0.1333 |
Tier II |
| 7 |
NC_000082.6 |
16 |
26971885 |
+ |
Gmnc |
NNNAGGTAGAGTGTGATCAG |
NGG |
2 |
0.1227 |
Tier II |
| 8 |
NC_000086.7 |
X |
141203985 |
+ |
Col4a6 |
NNNGGGTAGATTCTGATCAG |
NAG |
2 |
0.0997 |
Tier II |
| 9 |
NC_000082.6 |
16 |
28245682 |
- |
Gm52250 |
NNNGAGTAGAGTTTGATCAG |
NGG |
2 |
0.6067 |
Tier III |
| 10 |
NC_000077.6 |
11 |
25334021 |
- |
4933427E13Rik |
NNNGGGTAGAGTCTGATCAT |
NGT |
2 |
0.0113 |
Tier III |
Other clones with same target sequence:
(none)
