Construct: sgRNA BRDN0001147403

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCGCTGTGCCAATGCCTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TYRO3 (7301)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76229

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 41567486 + TYRO3 NNNGCTGTGCCAATGCCTTG NGG 0 1.0 Tier I
2 NC_000001.11 1 209623114 + LAMB3 NNNGCTGTGCCATTGTCTTG NGG 2 0.0923 Tier I
3 NC_000011.10 11 64352267 - CCDC88B NNNGCTGTGCCAGGGCCTTG NGG 2 0.0 Tier I
4 NC_000019.10 19 45076662 - GEMIN7 NNNGCTGTTTCAATGCCTTG NGG 2 0.502 Tier II
5 NC_000015.10 15 76260798 - ETFA NNNGCTGTGCCAATGCTTTG NGG 1 0.4667 Tier II
6 NC_000008.11 8 27887602 - SCARA5 NNNGCTCTGCCAAAGCCTTG NGG 2 0.4256 Tier II
7 NC_000009.12 9 132800860 - AK8 NNNGCTGTGTCAATGCCGTG NGG 2 0.3137 Tier II
8 NC_000017.11 17 45082028 + NMT1 NNNGCTGTGCCAGTGCCCTG NGG 2 0.2795 Tier II
9 NC_000022.11 22 43967286 - SAMM50 NNNGCTGTGGCAATGCCATG NGG 2 0.2593 Tier II
10 NC_000010.11 10 32806956 - CCDC7 NNNGCTGTCCCAATGCCTTC NGG 2 0.2308 Tier II
11 NC_000002.12 2 95338098 - KCNIP3 NNNGCTGTGCCGATGCCTCG NGG 2 0.1992 Tier II
12 NC_000009.12 9 9999068 - PTPRD NNNGTTGTGCTAATGCCTTG NGG 2 0.1958 Tier II
13 NC_000003.12 3 159484786 - SCHIP1 NNNTCTGTGCCAATGCATTG NGG 2 0.1697 Tier II
14 NC_000003.12 3 159484786 - IQCJ-SCHIP1 NNNTCTGTGCCAATGCATTG NGG 2 0.1697 Tier II
15 NC_000002.12 2 233743942 - UGT1A10 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
16 NC_000002.12 2 233743942 - UGT1A8 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
17 NC_000002.12 2 233743942 - UGT1A7 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
18 NC_000002.12 2 233743942 - UGT1A6 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
19 NC_000002.12 2 233743942 - UGT1A5 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
20 NC_000002.12 2 233743942 - UGT1A9 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
21 NC_000002.12 2 233743942 - UGT1A4 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
22 NC_000002.12 2 233743942 - UGT1A3 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier II
23 NC_000018.10 18 27975469 + CDH2 NNNGCTGAGCCAATTCCTTG NGG 2 0.1143 Tier II
24 NC_000011.10 11 19411048 - NAV2 NNNGCTGTGCCAATGCCCTG NAG 2 0.1111 Tier II
25 NC_000004.12 4 153718439 + RNF175 NNNGCTGTACCAATGGCTTG NGG 2 0.0989 Tier II
26 NC_000017.11 17 45708944 - LINC02210-CRHR1 NNNGCTGTGCCCTTGCCTTG NGG 2 0.0789 Tier II
27 NC_000002.12 2 214578298 - VWC2L NNNGCTGTGCCAATGCTTTG NTG 2 0.0182 Tier II
28 NC_000002.12 2 233743942 - UGT1A NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier III
29 NC_000002.12 2 233743942 - DNAJB3 NNNGCTGTGCCAGTGCCTGG NGG 2 0.163 Tier III
30 NC_000008.11 8 57503043 + LOC105375855 NNNGCTGTGCCAATTCCTTT NGG 2 0.1 Tier III
31 NC_000009.12 9 117443848 - LOC105376242 NNNTCTGTGCCAATGGCTTG NGG 2 0.0559 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 121613545 - Xpot NNNGCTGTGCCAGTGCCTTG NAG 2 0.1691 Tier I
2 NC_000068.7 2 119807961 + Tyro3 NNNGCTGTGCCAATGCCTTG NGC 1 0.0222 Tier I
3 NC_000082.6 16 35561756 - Sema5b NNNGCTGGGCCAATGCCTTG NGG 1 0.7333 Tier II
4 NC_000070.6 4 149179175 - Kif1b NNNGCTGTGCCAGTGACTTG NGG 2 0.6522 Tier II
5 NC_000084.6 18 13767360 - Zfp521 NNNGTTCTGCCAATGCCTTG NGG 2 0.4375 Tier II
6 NC_000074.6 8 68017261 - Psd3 NNNGCTGTGACAATGCTTTG NGG 2 0.4044 Tier II
7 NC_000067.6 1 158510638 + Astn1 NNNTCAGTGCCAATGCCTTG NGG 2 0.3152 Tier II
8 NC_000070.6 4 61525647 - Mup-ps12 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier II
9 NC_000070.6 4 61788902 - Mup-ps16 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier II
10 NC_000070.6 4 62022520 - Mup20 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier II
11 NC_000069.6 3 121209912 + Tlcd4 NNNGCTGTGCCCATGCCTTA NGG 2 0.2467 Tier II
12 NC_000071.6 5 26959434 - Dpp6 NNNGGTGTGCCTATGCCTTG NGG 2 0.2 Tier II
13 NC_000075.6 9 99429039 - Mras NNNGCTGTCCCAATGCCTGG NGG 2 0.1346 Tier II
14 NC_000085.6 19 32346339 - Sgms1 NNNGCTGTGCCTTTGCCTTG NGG 2 0.1 Tier II
15 NC_000068.7 2 169693388 - Tshz2 NNNGCTGTGCCAATGCCTGG NAG 2 0.0648 Tier II
16 NC_000074.6 8 57536153 + Galnt7 NNNGCTGTGCCAATGCCCTG NGT 2 0.0069 Tier II
17 NC_000078.6 12 11393544 + Vsnl1 NNNGCTGTGCCCATGCCTTG NGT 2 0.0042 Tier II
18 NC_000076.6 10 24959594 + Gm36228 NNNGCTGTGCCAATGATTTG NGG 2 0.4667 Tier III
19 NC_000074.6 8 68017261 - Gm15991 NNNGCTGTGACAATGCTTTG NGG 2 0.4044 Tier III
20 NC_000067.6 1 158510638 + Gm37679 NNNTCAGTGCCAATGCCTTG NGG 2 0.3152 Tier III
21 NC_000070.6 4 60077224 - Mup-ps1 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier III
22 NC_000070.6 4 61234934 - Mup-ps10 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier III
23 NC_000070.6 4 61691066 - Mup-ps15 NNNGCTGTGCCATTGCCTTA NGG 2 0.2813 Tier III
24 NC_000074.6 8 3995263 + Gm7389 NNNGCTGTGCCAGTGCCTTG NAG 2 0.1691 Tier III
25 NC_000068.7 2 118898445 - Gm32957 NNNGCTGTGCCACTGCCTGG NGG 2 0.0526 Tier III
26 NC_000067.6 1 137353842 + Gm34244 NNNGCTGTGCCAAGGCCTTG NAG 2 0.0 Tier III
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Other clones with same target sequence:

(none)