Construct: sgRNA BRDN0001147403
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCGCTGTGCCAATGCCTTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- TYRO3 (7301)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000015.10 | 15 | 41567486 | + | TYRO3 | NNNGCTGTGCCAATGCCTTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 209623114 | + | LAMB3 | NNNGCTGTGCCATTGTCTTG | NGG | 2 | 0.0923 | Tier I |
3 | NC_000011.10 | 11 | 64352267 | - | CCDC88B | NNNGCTGTGCCAGGGCCTTG | NGG | 2 | 0.0 | Tier I |
4 | NC_000019.10 | 19 | 45076662 | - | GEMIN7 | NNNGCTGTTTCAATGCCTTG | NGG | 2 | 0.502 | Tier II |
5 | NC_000015.10 | 15 | 76260798 | - | ETFA | NNNGCTGTGCCAATGCTTTG | NGG | 1 | 0.4667 | Tier II |
6 | NC_000008.11 | 8 | 27887602 | - | SCARA5 | NNNGCTCTGCCAAAGCCTTG | NGG | 2 | 0.4256 | Tier II |
7 | NC_000009.12 | 9 | 132800860 | - | AK8 | NNNGCTGTGTCAATGCCGTG | NGG | 2 | 0.3137 | Tier II |
8 | NC_000017.11 | 17 | 45082028 | + | NMT1 | NNNGCTGTGCCAGTGCCCTG | NGG | 2 | 0.2795 | Tier II |
9 | NC_000022.11 | 22 | 43967286 | - | SAMM50 | NNNGCTGTGGCAATGCCATG | NGG | 2 | 0.2593 | Tier II |
10 | NC_000010.11 | 10 | 32806956 | - | CCDC7 | NNNGCTGTCCCAATGCCTTC | NGG | 2 | 0.2308 | Tier II |
11 | NC_000002.12 | 2 | 95338098 | - | KCNIP3 | NNNGCTGTGCCGATGCCTCG | NGG | 2 | 0.1992 | Tier II |
12 | NC_000009.12 | 9 | 9999068 | - | PTPRD | NNNGTTGTGCTAATGCCTTG | NGG | 2 | 0.1958 | Tier II |
13 | NC_000003.12 | 3 | 159484786 | - | SCHIP1 | NNNTCTGTGCCAATGCATTG | NGG | 2 | 0.1697 | Tier II |
14 | NC_000003.12 | 3 | 159484786 | - | IQCJ-SCHIP1 | NNNTCTGTGCCAATGCATTG | NGG | 2 | 0.1697 | Tier II |
15 | NC_000002.12 | 2 | 233743942 | - | UGT1A10 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
16 | NC_000002.12 | 2 | 233743942 | - | UGT1A8 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
17 | NC_000002.12 | 2 | 233743942 | - | UGT1A7 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
18 | NC_000002.12 | 2 | 233743942 | - | UGT1A6 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
19 | NC_000002.12 | 2 | 233743942 | - | UGT1A5 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
20 | NC_000002.12 | 2 | 233743942 | - | UGT1A9 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
21 | NC_000002.12 | 2 | 233743942 | - | UGT1A4 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
22 | NC_000002.12 | 2 | 233743942 | - | UGT1A3 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier II |
23 | NC_000018.10 | 18 | 27975469 | + | CDH2 | NNNGCTGAGCCAATTCCTTG | NGG | 2 | 0.1143 | Tier II |
24 | NC_000011.10 | 11 | 19411048 | - | NAV2 | NNNGCTGTGCCAATGCCCTG | NAG | 2 | 0.1111 | Tier II |
25 | NC_000004.12 | 4 | 153718439 | + | RNF175 | NNNGCTGTACCAATGGCTTG | NGG | 2 | 0.0989 | Tier II |
26 | NC_000017.11 | 17 | 45708944 | - | LINC02210-CRHR1 | NNNGCTGTGCCCTTGCCTTG | NGG | 2 | 0.0789 | Tier II |
27 | NC_000002.12 | 2 | 214578298 | - | VWC2L | NNNGCTGTGCCAATGCTTTG | NTG | 2 | 0.0182 | Tier II |
28 | NC_000002.12 | 2 | 233743942 | - | UGT1A | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier III |
29 | NC_000002.12 | 2 | 233743942 | - | DNAJB3 | NNNGCTGTGCCAGTGCCTGG | NGG | 2 | 0.163 | Tier III |
30 | NC_000008.11 | 8 | 57503043 | + | LOC105375855 | NNNGCTGTGCCAATTCCTTT | NGG | 2 | 0.1 | Tier III |
31 | NC_000009.12 | 9 | 117443848 | - | LOC105376242 | NNNTCTGTGCCAATGGCTTG | NGG | 2 | 0.0559 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000076.6 | 10 | 121613545 | - | Xpot | NNNGCTGTGCCAGTGCCTTG | NAG | 2 | 0.1691 | Tier I |
2 | NC_000068.7 | 2 | 119807961 | + | Tyro3 | NNNGCTGTGCCAATGCCTTG | NGC | 1 | 0.0222 | Tier I |
3 | NC_000082.6 | 16 | 35561756 | - | Sema5b | NNNGCTGGGCCAATGCCTTG | NGG | 1 | 0.7333 | Tier II |
4 | NC_000070.6 | 4 | 149179175 | - | Kif1b | NNNGCTGTGCCAGTGACTTG | NGG | 2 | 0.6522 | Tier II |
5 | NC_000084.6 | 18 | 13767360 | - | Zfp521 | NNNGTTCTGCCAATGCCTTG | NGG | 2 | 0.4375 | Tier II |
6 | NC_000074.6 | 8 | 68017261 | - | Psd3 | NNNGCTGTGACAATGCTTTG | NGG | 2 | 0.4044 | Tier II |
7 | NC_000067.6 | 1 | 158510638 | + | Astn1 | NNNTCAGTGCCAATGCCTTG | NGG | 2 | 0.3152 | Tier II |
8 | NC_000070.6 | 4 | 61525647 | - | Mup-ps12 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier II |
9 | NC_000070.6 | 4 | 61788902 | - | Mup-ps16 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier II |
10 | NC_000070.6 | 4 | 62022520 | - | Mup20 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier II |
11 | NC_000069.6 | 3 | 121209912 | + | Tlcd4 | NNNGCTGTGCCCATGCCTTA | NGG | 2 | 0.2467 | Tier II |
12 | NC_000071.6 | 5 | 26959434 | - | Dpp6 | NNNGGTGTGCCTATGCCTTG | NGG | 2 | 0.2 | Tier II |
13 | NC_000075.6 | 9 | 99429039 | - | Mras | NNNGCTGTCCCAATGCCTGG | NGG | 2 | 0.1346 | Tier II |
14 | NC_000085.6 | 19 | 32346339 | - | Sgms1 | NNNGCTGTGCCTTTGCCTTG | NGG | 2 | 0.1 | Tier II |
15 | NC_000068.7 | 2 | 169693388 | - | Tshz2 | NNNGCTGTGCCAATGCCTGG | NAG | 2 | 0.0648 | Tier II |
16 | NC_000074.6 | 8 | 57536153 | + | Galnt7 | NNNGCTGTGCCAATGCCCTG | NGT | 2 | 0.0069 | Tier II |
17 | NC_000078.6 | 12 | 11393544 | + | Vsnl1 | NNNGCTGTGCCCATGCCTTG | NGT | 2 | 0.0042 | Tier II |
18 | NC_000076.6 | 10 | 24959594 | + | Gm36228 | NNNGCTGTGCCAATGATTTG | NGG | 2 | 0.4667 | Tier III |
19 | NC_000074.6 | 8 | 68017261 | - | Gm15991 | NNNGCTGTGACAATGCTTTG | NGG | 2 | 0.4044 | Tier III |
20 | NC_000067.6 | 1 | 158510638 | + | Gm37679 | NNNTCAGTGCCAATGCCTTG | NGG | 2 | 0.3152 | Tier III |
21 | NC_000070.6 | 4 | 60077224 | - | Mup-ps1 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier III |
22 | NC_000070.6 | 4 | 61234934 | - | Mup-ps10 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier III |
23 | NC_000070.6 | 4 | 61691066 | - | Mup-ps15 | NNNGCTGTGCCATTGCCTTA | NGG | 2 | 0.2813 | Tier III |
24 | NC_000074.6 | 8 | 3995263 | + | Gm7389 | NNNGCTGTGCCAGTGCCTTG | NAG | 2 | 0.1691 | Tier III |
25 | NC_000068.7 | 2 | 118898445 | - | Gm32957 | NNNGCTGTGCCACTGCCTGG | NGG | 2 | 0.0526 | Tier III |
26 | NC_000067.6 | 1 | 137353842 | + | Gm34244 | NNNGCTGTGCCAAGGCCTTG | NAG | 2 | 0.0 | Tier III |