Construct: sgRNA BRDN0001147410

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTCACTCGCACCTCACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFN1 (91156)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76823

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 201208480 + IGFN1 NNNACTCGCACCTCACAATG NGG 0 1.0 Tier I
2 NC_000022.11 22 46609694 - GRAMD4 NNNACTCGCACTCCACAATG NGG 2 0.4251 Tier II
3 NC_000002.12 2 158113285 + UPP2 NNNACTGGCACCACACAATG NGG 2 0.3258 Tier II
4 NC_000004.12 4 1726170 - TACC3 NNNACTCGCTGCTCACAATG NGG 2 0.2206 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 6756363 + Rbfox1 NNNACCCGCTCCTCACAATG NGG 2 0.8021 Tier II
2 NC_000070.6 4 128561332 + Csmd2 NNNACTCGGACCTCACAGTG NGG 2 0.2476 Tier II
3 NC_000077.6 11 113923974 - Sdk2 NNNCCTGGCACCTCACAATG NGG 2 0.1661 Tier II
4 NC_000082.6 16 90257016 - Scaf4 NNNACTCGTACCTCACAATG NTG 2 0.0341 Tier II
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Other clones with same target sequence:

(none)