Construct: sgRNA BRDN0001147423
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGATACTACATTACCACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FYN (2534)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77244
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
111702933 |
- |
FYN |
NNNATACTACATTACCACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
49323751 |
+ |
CAB39L |
NNNATACTATATTACTACCC |
NGG |
2 |
0.2896 |
Tier II |
| 3 |
NC_000015.10 |
15 |
38470693 |
- |
FAM98B |
NNNATACTACATTAAAACCC |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000003.12 |
3 |
59014993 |
+ |
CFAP20DC |
NNNATACTCCATTACCACCC |
NAG |
2 |
0.1481 |
Tier II |
| 5 |
NC_000003.12 |
3 |
142548222 |
- |
ATR |
NNNATACTACATTTCCTCCC |
NGG |
2 |
0.0711 |
Tier II |
| 6 |
NC_000019.10 |
19 |
31591822 |
- |
LINC03103 |
NNNATCCTGCATTACCACCC |
NGG |
2 |
0.303 |
Tier III |
| 7 |
NC_000005.10 |
5 |
17455922 |
- |
LINC02218 |
NNNATACTACAGTACCACCT |
NGG |
2 |
0.15 |
Tier III |
| 8 |
NC_000003.12 |
3 |
59014993 |
+ |
CFAP20DC-AS1 |
NNNATACTCCATTACCACCC |
NAG |
2 |
0.1481 |
Tier III |
| 9 |
NC_000002.12 |
2 |
219366979 |
+ |
LOC105373883 |
NNNATAGTACATTACCAGCC |
NGG |
2 |
0.0627 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
73944852 |
- |
Brd9 |
NNNATACTACCTTGCCACCC |
NGG |
2 |
0.1867 |
Tier I |
| 2 |
NC_000083.6 |
17 |
26235388 |
+ |
Fam234a |
NNNATAAGACATTACCACCC |
NGG |
2 |
0.55 |
Tier II |
| 3 |
NC_000072.6 |
6 |
108853756 |
+ |
Edem1 |
NNNATAGTGCATTACCACCC |
NGG |
2 |
0.3137 |
Tier II |
| 4 |
NC_000085.6 |
19 |
44234582 |
- |
Scd3 |
NNNATACTACATTATAACCC |
NGG |
2 |
0.0667 |
Tier II |
| 5 |
NC_000085.6 |
19 |
44234582 |
- |
Pkd2l1 |
NNNATACTACATTATAACCC |
NGG |
2 |
0.0667 |
Tier II |
| 6 |
NC_000067.6 |
1 |
124178374 |
- |
Dpp10 |
NNNATACTAAATTACCACCC |
NTG |
2 |
0.0338 |
Tier II |
| 7 |
NC_000081.6 |
15 |
78791713 |
- |
Card10 |
NNNATACTTCATTACCACCC |
NGC |
2 |
0.0133 |
Tier II |
| 8 |
NC_000082.6 |
16 |
4169686 |
+ |
Crebbp |
NNNATACTACATTACCAGCC |
NGC |
2 |
0.003 |
Tier II |
| 9 |
NC_000077.6 |
11 |
80807561 |
- |
Gm31228 |
NNNGTACCACATTACCACCC |
NGG |
2 |
0.625 |
Tier III |
Other clones with same target sequence:
(none)
