Construct: sgRNA BRDN0001147429
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTAACCCTCTCACAGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CG (5294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76333
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000007.14 |
7 |
106869267 |
+ |
PIK3CG |
NNNTAACCCTCTCACAGCAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000021.9 |
21 |
46353274 |
+ |
PCNT |
NNNTAACCCTGTTACAGCAG |
NGG |
2 |
0.175 |
Tier I |
3 |
NC_000007.14 |
7 |
111895346 |
+ |
DOCK4 |
NNNTAACCCTATCACAGCAA |
NGG |
2 |
0.7031 |
Tier II |
4 |
NC_000001.11 |
1 |
223247529 |
+ |
SUSD4 |
NNNTAACCTTCTTACAGCAG |
NGG |
2 |
0.6125 |
Tier II |
5 |
NC_000011.10 |
11 |
66866884 |
- |
PC |
NNNCAACCCTCTCACAGCTG |
NGG |
2 |
0.3484 |
Tier II |
6 |
NC_000006.12 |
6 |
79940693 |
+ |
ELOVL4 |
NNNCAACCCTCTCACAGCAC |
NGG |
2 |
0.2773 |
Tier II |
7 |
NC_000010.11 |
10 |
132322033 |
+ |
STK32C |
NNNACACCCTCTCACAGCAG |
NGG |
2 |
0.2381 |
Tier II |
8 |
NC_000001.11 |
1 |
19140725 |
+ |
UBR4 |
NNNTTACACTCTCACAGCAG |
NGG |
2 |
0.2364 |
Tier II |
9 |
NC_000003.12 |
3 |
11644455 |
+ |
VGLL4 |
NNNTCACCCTTTCACAGCAG |
NGG |
2 |
0.1538 |
Tier II |
10 |
NC_000006.12 |
6 |
93359129 |
- |
EPHA7 |
NNNTAACCCTCTCTCGGCAG |
NGG |
2 |
0.1026 |
Tier II |
11 |
NC_000009.12 |
9 |
136045395 |
- |
NACC2 |
NNNTAACCCTTTCCCAGCAG |
NGG |
2 |
0.0659 |
Tier II |
12 |
NC_000007.14 |
7 |
139297494 |
+ |
UBN2 |
NNNTAACCCTCTCACAGCAT |
NTG |
2 |
0.0273 |
Tier II |
13 |
NC_000006.12 |
6 |
52564839 |
+ |
TRAM2 |
NNNTAACCCTCTCAGGGCAG |
NGG |
2 |
0.0096 |
Tier II |
14 |
NC_000012.12 |
12 |
25109342 |
+ |
DNAI7 |
NNNTAACCCTCTCAGAGGAG |
NGG |
2 |
0.0067 |
Tier II |
15 |
NC_000003.12 |
3 |
13601791 |
- |
FBLN2 |
NNNTAACCCTCTCACAGCAG |
NCA |
2 |
0.0 |
Tier II |
16 |
NC_000009.12 |
9 |
127754514 |
+ |
SH2D3C |
NNNTAACCCTCTCACCGCCG |
NGG |
2 |
0.0 |
Tier II |
17 |
NC_000005.10 |
5 |
21022867 |
+ |
LOC105374678 |
NNNTAAACCTCTCACAACAG |
NGG |
2 |
0.7 |
Tier III |
18 |
NC_000023.11 |
X |
69062467 |
+ |
LOC105373242 |
NNNTTGCCCTCTCACAGCAG |
NGG |
2 |
0.2597 |
Tier III |
19 |
NC_000003.12 |
3 |
34955832 |
- |
LOC101928135 |
NNNTCACCCTCTCACTGCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000078.6 |
12 |
32204282 |
- |
Pik3cg |
NNNTAACCCCCTCACAGCAG |
NGG |
1 |
0.5333 |
Tier I |
2 |
NC_000070.6 |
4 |
136542879 |
+ |
Luzp1 |
NNNTACCCCTCTGACAGCAG |
NGG |
2 |
0.062 |
Tier I |
3 |
NC_000076.6 |
10 |
80415524 |
- |
Tcf3 |
NNNTCACCCTCTCACAGCAG |
NGC |
2 |
0.0111 |
Tier I |
4 |
NC_000071.6 |
5 |
36172392 |
+ |
Sorcs2 |
NNNTAATCCTCTCACAGCAA |
NGG |
2 |
0.7617 |
Tier II |
5 |
NC_000067.6 |
1 |
154424622 |
+ |
Cacna1e |
NNNCAACCCTCCCACAGCAG |
NGG |
2 |
0.613 |
Tier II |
6 |
NC_000072.6 |
6 |
116400921 |
+ |
Marchf8 |
NNNTATTCCTCTCACAGCAG |
NGG |
2 |
0.5804 |
Tier II |
7 |
NC_000071.6 |
5 |
123455291 |
- |
Mlxip |
NNNCGACCCTCTCACAGCAG |
NGG |
2 |
0.4659 |
Tier II |
8 |
NC_000076.6 |
10 |
110045631 |
- |
Nav3 |
NNNCAACCCTCGCACAGCAG |
NGG |
2 |
0.3235 |
Tier II |
9 |
NC_000068.7 |
2 |
62678542 |
+ |
Gca |
NNNTTGCCCTCTCACAGCAG |
NGG |
2 |
0.2597 |
Tier II |
10 |
NC_000075.6 |
9 |
64449521 |
+ |
Megf11 |
NNNTCAGCCTCTCACAGCAG |
NGG |
2 |
0.2353 |
Tier II |
11 |
NC_000069.6 |
3 |
65683459 |
- |
Lekr1 |
NNNTGACCCTTTCACAGCAG |
NGG |
2 |
0.2215 |
Tier II |
12 |
NC_000083.6 |
17 |
42599917 |
- |
Opn5 |
NNNTCACCCTCTCACAGCAG |
NAG |
2 |
0.1296 |
Tier II |
13 |
NC_000081.6 |
15 |
57944612 |
+ |
Tbc1d31 |
NNNAAACCCTCTCACACCAG |
NGG |
2 |
0.112 |
Tier II |
14 |
NC_000076.6 |
10 |
70994670 |
+ |
Bicc1 |
NNNTAAGCCTCTCAAAGCAG |
NGG |
2 |
0.1046 |
Tier II |
15 |
NC_000067.6 |
1 |
190168335 |
+ |
Prox1 |
NNNAAACCCTCTCACGGCAG |
NGG |
2 |
0.0916 |
Tier II |
16 |
NC_000075.6 |
9 |
104095807 |
- |
Acad11 |
NNNGAACCCTCTCACAGGAG |
NGG |
2 |
0.0833 |
Tier II |
17 |
NC_000072.6 |
6 |
97118321 |
- |
Eogt |
NNNTAGCCCTCTCACAGCAG |
NTG |
2 |
0.0278 |
Tier II |
18 |
NC_000077.6 |
11 |
56048171 |
- |
LOC115485826 |
NNNTATCCCTCTCACAACAG |
NGG |
2 |
0.6667 |
Tier III |
Other clones with same target sequence:
(none)