Construct: sgRNA BRDN0001147432
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATATTTGGCTCGGCTGCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80186
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
110511126 |
- |
COL4A2 |
NNNATTTGGCTTGGCTGAGC |
NGG |
2 |
0.2899 |
Tier II |
| 2 |
NC_000012.12 |
12 |
99345805 |
- |
ANKS1B |
NNNATTTGGCTCAGCTCCGC |
NGG |
2 |
0.2172 |
Tier II |
| 3 |
NC_000020.11 |
20 |
8515820 |
+ |
PLCB1 |
NNNATTTGGCTCAGCTGGGC |
NGG |
2 |
0.1231 |
Tier II |
| 4 |
NC_000015.10 |
15 |
100093232 |
+ |
ADAMTS17 |
NNNATTTGGCTCTGCTGGGC |
NGG |
2 |
0.04 |
Tier II |
| 5 |
NC_000012.12 |
12 |
6534807 |
- |
GAPDH |
NNNATGTGGCTCGGCTGCGC |
NGA |
2 |
0.0397 |
Tier II |
| 6 |
NC_000004.12 |
4 |
3586887 |
+ |
LINC00955 |
NNNATTTGGCTGGGCTGGGC |
NGG |
2 |
0.0593 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28179878 |
+ |
Dyrk1b |
NNNTTTGGGCTCGGCTGCGC |
NGG |
2 |
0.3743 |
Tier II |
| 2 |
NC_000076.6 |
10 |
63953526 |
- |
Lrrtm3 |
NNNATTTGGCTCGGCTGCCC |
NTG |
2 |
0.0175 |
Tier II |
| 3 |
NC_000076.6 |
10 |
63953526 |
- |
Ctnna3 |
NNNATTTGGCTCGGCTGCCC |
NTG |
2 |
0.0175 |
Tier II |
Other clones with same target sequence:
(none)
