Construct: sgRNA BRDN0001147435
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGGTTCAGACATTGCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K14 (9020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77738
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
45286885 |
+ |
MAP3K14 |
NNNGGTTCAGACATTGCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
34729803 |
- |
RAI14 |
NNNGGTTCATACATTGCAAA |
NGG |
2 |
0.7617 |
Tier II |
| 3 |
NC_000016.10 |
16 |
69751543 |
- |
NOB1 |
NNNGGTTCATACATTGCAAT |
NGG |
2 |
0.5688 |
Tier II |
| 4 |
NC_000013.11 |
13 |
23581755 |
- |
TNFRSF19 |
NNNGGTTCAGAGATTGCAAT |
NGG |
2 |
0.3111 |
Tier II |
| 5 |
NC_000018.10 |
18 |
3527360 |
- |
DLGAP1 |
NNNGGTTCAGATATTGCATG |
NGG |
2 |
0.2899 |
Tier II |
| 6 |
NC_000002.12 |
2 |
15188012 |
- |
NBAS |
NNNAGTTCAGACTTTGCAAG |
NGG |
2 |
0.27 |
Tier II |
| 7 |
NC_000010.11 |
10 |
77051590 |
+ |
KCNMA1 |
NNNGGTTCAGAGATTGAAAG |
NGG |
2 |
0.2074 |
Tier II |
| 8 |
NC_000015.10 |
15 |
84391463 |
+ |
LOC105376722 |
NNNGGTTCACACATTGAAAG |
NGG |
2 |
0.1867 |
Tier II |
| 9 |
NC_000003.12 |
3 |
125087481 |
- |
SLC12A8 |
NNNTGTTCAGACATTGCTAG |
NGG |
2 |
0.1818 |
Tier II |
| 10 |
NC_000009.12 |
9 |
129869635 |
+ |
USP20 |
NNNGGTGCAGACATTGCCAG |
NGG |
2 |
0.112 |
Tier II |
| 11 |
NC_000016.10 |
16 |
87360762 |
+ |
FBXO31 |
NNNGGTTCAGACAGTGCCAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000012.12 |
12 |
88597553 |
- |
LOC105369885 |
NNNGGCTCATACATTGCAAG |
NGG |
2 |
0.7386 |
Tier III |
| 13 |
NC_000009.12 |
9 |
129869635 |
+ |
MIR6855 |
NNNGGTGCAGACATTGCCAG |
NGG |
2 |
0.112 |
Tier III |
| 14 |
NC_000010.11 |
10 |
8217446 |
- |
LOC107984205 |
NNNGGTTCAGACAGTTCAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
103239392 |
+ |
Map3k14 |
NNNGGTTTAGACATTGCAAG |
NGG |
1 |
0.875 |
Tier I |
| 2 |
NC_000076.6 |
10 |
83138604 |
+ |
Chst11 |
NNNGGTGCAGACATTGCAAG |
NGG |
1 |
0.5882 |
Tier II |
| 3 |
NC_000080.6 |
14 |
117865746 |
- |
Gpc6 |
NNNAGTTCAGACATTGCAGG |
NGG |
2 |
0.3375 |
Tier II |
| 4 |
NC_000085.6 |
19 |
24001422 |
+ |
Fam189a2 |
NNNGATTCAGTCATTGCAAG |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000084.6 |
18 |
63709567 |
- |
Wdr7 |
NNNGGTGCAGACATTGCAGG |
NGG |
2 |
0.2206 |
Tier II |
| 6 |
NC_000080.6 |
14 |
117579057 |
+ |
Gpc6 |
NNNGGTTCTGACATGGCAAG |
NGG |
2 |
0.03 |
Tier II |
| 7 |
NC_000079.6 |
13 |
14942906 |
- |
Gm48329 |
NNNGGCTCAGGCATTGCAAG |
NGG |
2 |
0.5909 |
Tier III |
| 8 |
NC_000076.6 |
10 |
83138604 |
+ |
LOC115487465 |
NNNGGTGCAGACATTGCAAG |
NGG |
1 |
0.5882 |
Tier III |
| 9 |
NC_000068.7 |
2 |
51688427 |
- |
Gm13490 |
NNNGGTTCAGACACTGCAAG |
NGG |
1 |
0.2857 |
Tier III |
| 10 |
NC_000074.6 |
8 |
113072930 |
+ |
Gm10280 |
NNNGGTTAAGTCATTGCAAG |
NGG |
2 |
0.2 |
Tier III |
Other clones with same target sequence:
(none)
