Construct: sgRNA BRDN0001147436
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCCAGTCGCAATTGCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAB1 (10454)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77791
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
39416873 |
+ |
TAB1 |
NNNCCAGTCGCAATTGCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
16688464 |
+ |
BZW2 |
NNNCCAGTAGCAATTGCCAG |
NGG |
1 |
0.8571 |
Tier II |
| 3 |
NC_000012.12 |
12 |
124851265 |
- |
SCARB1 |
NNNCCAGTCTCACTTGCCAG |
NGG |
2 |
0.1711 |
Tier II |
| 4 |
NC_000008.11 |
8 |
130379485 |
- |
ASAP1 |
NNNCCAGTCACAATTCCCAG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
20214488 |
+ |
Yeats2 |
NNNCCAGTAGCAATTGCCAA |
NGG |
2 |
0.8036 |
Tier I |
| 2 |
NC_000075.6 |
9 |
8548778 |
- |
Trpc6 |
NNNCCAGTTGAAATTGCCAG |
NGG |
2 |
0.6563 |
Tier II |
| 3 |
NC_000086.7 |
X |
13522559 |
- |
Cask |
NNNCCAGTCACAGTTGCCAG |
NGG |
2 |
0.6087 |
Tier II |
| 4 |
NC_000079.6 |
13 |
29796569 |
- |
Cdkal1 |
NNNCCAGTAGCTATTGCCAG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000082.6 |
16 |
9678278 |
+ |
Grin2a |
NNNCCAGTGGCAATTGCCAG |
NAG |
2 |
0.1605 |
Tier II |
| 6 |
NC_000067.6 |
1 |
144118505 |
+ |
Gm35356 |
NNNCCAGTCCAAATTGCCAG |
NGG |
2 |
0.3 |
Tier III |
Other clones with same target sequence:
(none)
