Construct: sgRNA BRDN0001147437
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGATGGGACGGTTGACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK1 (65125)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75623
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
862099 |
+ |
WNK1 |
NNNGATGGGACGGTTGACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
54199086 |
+ |
MLIP |
NNNGATGAGACAGTTGACAG |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000011.10 |
11 |
126277233 |
+ |
FOXRED1 |
NNNGATGGGACAGATGACAG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000001.11 |
1 |
171062719 |
- |
MROH9 |
NNNGATGGGACGATAGACAG |
NGG |
2 |
0.5344 |
Tier II |
| 5 |
NC_000009.12 |
9 |
91480935 |
+ |
NFIL3 |
NNNGATGGGACAGCTGACAG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000013.11 |
13 |
24281119 |
- |
SPATA13 |
NNNGAGGGGACGGTTGCCAG |
NGG |
2 |
0.1008 |
Tier II |
| 7 |
NC_000020.11 |
20 |
15277137 |
- |
MACROD2 |
NNNGATGGGATGGTTGCCAG |
NGG |
2 |
0.0543 |
Tier II |
| 8 |
NC_000018.10 |
18 |
48401904 |
- |
ZBTB7C |
NNNGATGGGACGGTTGACTG |
NGA |
2 |
0.0374 |
Tier II |
| 9 |
NC_000002.12 |
2 |
109140121 |
- |
RANBP2 |
NNNGAGGGGACGGTGGACAG |
NGG |
2 |
0.0286 |
Tier II |
| 10 |
NC_000002.12 |
2 |
109140121 |
- |
SH3RF3 |
NNNGAGGGGACGGTGGACAG |
NGG |
2 |
0.0286 |
Tier II |
| 11 |
NC_000012.12 |
12 |
52630257 |
+ |
KRT73 |
NNNGATGGGAAGGTTGACAG |
NGT |
2 |
0.0121 |
Tier II |
| 12 |
NC_000020.11 |
20 |
58610733 |
- |
APCDD1L-DT |
NNNGAGGGGACGGTTGACTG |
NGG |
2 |
0.3077 |
Tier III |
| 13 |
NC_000003.12 |
3 |
45336563 |
+ |
LOC105377061 |
NNNGATGGGACTGTTCACAG |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000003.12 |
3 |
45336563 |
+ |
LOC105377062 |
NNNGATGGGACTGTTCACAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
127396998 |
+ |
Mgat5 |
NNNGGTGGGAAGGTTGACAG |
NGG |
2 |
0.54 |
Tier II |
| 2 |
NC_000074.6 |
8 |
116586422 |
+ |
Cdyl2 |
NNNGATGGGACGCTTGAAAG |
NGG |
2 |
0.2267 |
Tier II |
| 3 |
NC_000074.6 |
8 |
3270039 |
+ |
Insr |
NNNGATGTGATGGTTGACAG |
NGG |
2 |
0.1923 |
Tier II |
| 4 |
NC_000077.6 |
11 |
74872962 |
- |
Sgsm2 |
NNNGATGGGATGGATGACAG |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000068.7 |
2 |
141566877 |
+ |
Macrod2 |
NNNGATGGGACAGTTGCCAG |
NGG |
2 |
0.1647 |
Tier II |
| 6 |
NC_000071.6 |
5 |
36941194 |
+ |
Ppp2r2c |
NNNGATGGGAAGGTTGGCAG |
NGG |
2 |
0.1324 |
Tier II |
| 7 |
NC_000068.7 |
2 |
3195419 |
- |
Fam171a1 |
NNNGATGGGAAGGTTGACAG |
NGA |
2 |
0.0521 |
Tier II |
| 8 |
NC_000081.6 |
15 |
7177647 |
+ |
Lifr |
NNNGATGGGAGGGTTGACAG |
NGA |
2 |
0.0174 |
Tier II |
| 9 |
NC_000071.6 |
5 |
125098045 |
+ |
Ncor2 |
NNNGATGGGACGGGTGACAG |
NGT |
2 |
0.0 |
Tier II |
| 10 |
NC_000068.7 |
2 |
58482366 |
+ |
Acvr1 |
NNNGATGGTACGGTTCACAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000067.6 |
1 |
127396998 |
+ |
Gm51746 |
NNNGGTGGGAAGGTTGACAG |
NGG |
2 |
0.54 |
Tier III |
| 12 |
NC_000076.6 |
10 |
70296647 |
- |
Gm40687 |
NNNGCTGGGAAGGTTGACAG |
NGG |
2 |
0.375 |
Tier III |
| 13 |
NC_000071.6 |
5 |
36941194 |
+ |
Gm52813 |
NNNGATGGGAAGGTTGGCAG |
NGG |
2 |
0.1324 |
Tier III |
Other clones with same target sequence:
(none)
