Construct: sgRNA BRDN0001147441
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTTGGCTGAGATTGGCGCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1 (5631)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76200
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
107640910 |
- |
PRPS1 |
NNNTGGCTGAGATTGGCGCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
196480097 |
- |
HECW2 |
NNNTGGCTGAGATTGGAACC |
NGG |
2 |
0.3231 |
Tier II |
| 3 |
NC_000021.9 |
21 |
42093310 |
- |
UMODL1 |
NNNAGGCTGAGATTGGAGCC |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000015.10 |
15 |
88085532 |
- |
NTRK3 |
NNNTGGCTGAGTTTGGCTCC |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000012.12 |
12 |
94429618 |
- |
CEP83 |
NNNTGGCTGAGATTGGAGCC |
NGA |
2 |
0.0324 |
Tier II |
| 6 |
NC_000009.12 |
9 |
127706539 |
+ |
PTRH1 |
NNNTGGCTGAGATTGGGGCT |
NGG |
2 |
0.0176 |
Tier II |
| 7 |
NC_000005.10 |
5 |
175675671 |
- |
HRH2 |
NNNTGGCTGAGATGGGCTCC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000010.11 |
10 |
78636931 |
+ |
LOC105378379 |
NNNTGGCTGAGGTTGGCTCC |
NGG |
2 |
0.4815 |
Tier III |
| 9 |
NC_000012.12 |
12 |
20885 |
- |
WASH8P |
NNNTGGCTGAGACTGGTGCC |
NGG |
2 |
0.3684 |
Tier III |
| 10 |
NC_000020.11 |
20 |
1354357 |
+ |
SDCBP2-AS1 |
NNNTGTCTGAGATTGGCCCC |
NGG |
2 |
0.3175 |
Tier III |
| 11 |
NC_000020.11 |
20 |
1354357 |
+ |
FKBP1A-SDCBP2 |
NNNTGTCTGAGATTGGCCCC |
NGG |
2 |
0.3175 |
Tier III |
| 12 |
NC_000005.10 |
5 |
68819246 |
- |
LOC105379013 |
NNNTGGCTGAGTTTGGCACC |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
57238740 |
- |
Prps1l3 |
NNNTAGCTGAGATTGGAGCC |
NGG |
2 |
0.4044 |
Tier I |
| 2 |
NC_000086.7 |
X |
140467680 |
- |
Prps1 |
NNNTAGCTGAGATTGGAGCC |
NGG |
2 |
0.4044 |
Tier I |
| 3 |
NC_000070.6 |
4 |
62467786 |
- |
Wdr31 |
NNNTAGCTGAGATTGGCTCC |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000076.6 |
10 |
73337853 |
+ |
Pcdh15 |
NNNTGGCTGAGAATGGCTCC |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000078.6 |
12 |
57238740 |
- |
Mipol1 |
NNNTAGCTGAGATTGGAGCC |
NGG |
2 |
0.4044 |
Tier II |
| 6 |
NC_000085.6 |
19 |
26698578 |
- |
Smarca2 |
NNNTGGCTGAGATTGGCGCT |
NGG |
1 |
0.3 |
Tier II |
| 7 |
NC_000069.6 |
3 |
154793762 |
- |
Tnni3k |
NNNTGGCTGACATTGGTGCC |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000070.6 |
4 |
43046150 |
+ |
Fam214b |
NNNTGGCTGTGATTGGCGCC |
NGC |
2 |
0.0196 |
Tier II |
| 9 |
NC_000083.6 |
17 |
29147836 |
+ |
Rab44 |
NNNTGGCTGAGATGGGCGGC |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
