Construct: sgRNA BRDN0001147449
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGTATCAGATGCACTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIB4 (130106)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75862
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
26641284 |
- |
CIB4 |
NNNGTATCAGATGCACTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
66634622 |
- |
VCPIP1 |
NNNGTAGCAGATGCACTGGG |
NGC |
2 |
0.0131 |
Tier I |
| 3 |
NC_000009.12 |
9 |
94915011 |
- |
AOPEP |
NNNGTATCAGAAGCACCGGG |
NGG |
2 |
0.5647 |
Tier II |
| 4 |
NC_000007.14 |
7 |
149975102 |
- |
ACTR3C |
NNNGTTTCAGGTGCACTGGG |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000007.14 |
7 |
154121733 |
+ |
DPP6 |
NNNGTTTCAGGTGCACTGGG |
NGG |
2 |
0.4643 |
Tier II |
| 6 |
NC_000009.12 |
9 |
35756272 |
- |
RGP1 |
NNNGTATCTGATGCACTAGG |
NGG |
2 |
0.4154 |
Tier II |
| 7 |
NC_000002.12 |
2 |
72802940 |
- |
EXOC6B |
NNNGTATCAGAACCACTGGG |
NGG |
2 |
0.3368 |
Tier II |
| 8 |
NC_000005.10 |
5 |
167774883 |
- |
TENM2 |
NNNTTAACAGATGCACTGGG |
NGG |
2 |
0.3182 |
Tier II |
| 9 |
NC_000003.12 |
3 |
40132615 |
- |
MYRIP |
NNNGTATCAGATTCACTGAG |
NGG |
2 |
0.2143 |
Tier II |
| 10 |
NC_000015.10 |
15 |
22791146 |
+ |
NIPA1 |
NNNGTATCAGTGGCACTGGG |
NGG |
2 |
0.1538 |
Tier II |
| 11 |
NC_000004.12 |
4 |
110109984 |
- |
ELOVL6 |
NNNGTATCAGCTGAACTGGG |
NGG |
2 |
0.14 |
Tier II |
| 12 |
NC_000001.11 |
1 |
235957522 |
- |
LINC02768 |
NNNGCATCAGATGCACTTGG |
NGG |
2 |
0.6667 |
Tier III |
| 13 |
NC_000001.11 |
1 |
235957522 |
- |
LOC105373215 |
NNNGCATCAGATGCACTTGG |
NGG |
2 |
0.6667 |
Tier III |
| 14 |
NC_000007.14 |
7 |
52298697 |
+ |
LOC124901810 |
NNNGTATCAGGTGCATTGGG |
NGG |
2 |
0.2 |
Tier III |
| 15 |
NC_000020.11 |
20 |
18809459 |
- |
SCP2D1-AS1 |
NNNGTATCAGATGCAGTGAG |
NGG |
2 |
0.1099 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
30545749 |
- |
Cib4 |
NNNGTACCAGATGCAGTGGG |
NGG |
2 |
0.1058 |
Tier I |
| 2 |
NC_000069.6 |
3 |
28702103 |
- |
Slc2a2 |
NNNGGACCAGATGCACTGGG |
NGG |
2 |
0.44 |
Tier II |
| 3 |
NC_000076.6 |
10 |
81117646 |
+ |
Map2k2 |
NNNGGACCAGATGCACTGGG |
NGG |
2 |
0.44 |
Tier II |
| 4 |
NC_000075.6 |
9 |
45178462 |
- |
Tmprss4 |
NNNGCATCAGATGCTCTGGG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000069.6 |
3 |
99288469 |
- |
Tbx15 |
NNNGTATCAGATCCCCTGGG |
NGG |
2 |
0.1148 |
Tier II |
| 6 |
NC_000069.6 |
3 |
99215640 |
- |
Wars2 |
NNNGTTTCAGATGCAGTGGG |
NGG |
2 |
0.1099 |
Tier II |
| 7 |
NC_000084.6 |
18 |
53264366 |
+ |
Snx24 |
NNNGTATCAGATGCAGAGGG |
NGG |
2 |
0.0821 |
Tier II |
| 8 |
NC_000076.6 |
10 |
122044919 |
+ |
Srgap1 |
NNNGTATCAGATGCAGTGGC |
NGG |
2 |
0.0659 |
Tier II |
| 9 |
NC_000069.6 |
3 |
139439633 |
+ |
Stpg2 |
NNNGTATCAGGTGCACTGGG |
NGA |
2 |
0.0451 |
Tier II |
| 10 |
NC_000072.6 |
6 |
147195703 |
+ |
E330012B07Rik |
NNNGTATGAGATCCACTGGG |
NGG |
2 |
0.2707 |
Tier III |
| 11 |
NC_000071.6 |
5 |
25174864 |
+ |
Gm33836 |
NNNGTATCAGATCCACTGGG |
NTG |
2 |
0.0164 |
Tier III |
| 12 |
NC_000081.6 |
15 |
63435566 |
- |
Gm20740 |
NNNGTATCAGATGGAATGGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
