Construct: sgRNA BRDN0001147450
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTCCAAATAGTAACTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK7 (1022)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76080
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
69262222 |
- |
CDK7 |
NNNTCCAAATAGTAACTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
84723520 |
+ |
DLG2 |
NNNTCCAAATAATAACTCTG |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000010.11 |
10 |
60130893 |
- |
ANK3 |
NNNTCAAAATAGTAACTTGG |
NGG |
2 |
0.5969 |
Tier II |
| 4 |
NC_000011.10 |
11 |
48037156 |
- |
PTPRJ |
NNNTCCAAATAGGAACTCCG |
NGG |
2 |
0.1169 |
Tier II |
| 5 |
NC_000015.10 |
15 |
25785211 |
- |
ATP10A |
NNNTCCAAATAGTCTCTCGG |
NGG |
2 |
0.0429 |
Tier II |
| 6 |
NC_000002.12 |
2 |
157210995 |
+ |
CDK7P1 |
NNNTCCAAATAGTAACTCAG |
NGG |
1 |
0.7143 |
Tier III |
| 7 |
NC_000011.10 |
11 |
84723520 |
+ |
LOC124902727 |
NNNTCCAAATAATAACTCTG |
NGG |
2 |
0.6222 |
Tier III |
| 8 |
NC_000015.10 |
15 |
87664430 |
- |
LOC102724465 |
NNNTCCAAATGGTAATTCGG |
NGG |
2 |
0.2 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
35010526 |
- |
Mob3b |
NNNTCCAGATAGTAACTCAG |
NGG |
2 |
0.5238 |
Tier II |
| 2 |
NC_000073.6 |
7 |
137273738 |
+ |
Ebf3 |
NNNTCCAAATGGTAACTGGG |
NGG |
2 |
0.0867 |
Tier II |
Other clones with same target sequence:
(none)
