Construct: sgRNA BRDN0001147455
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAACTGGCTAATGGTTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CPNE3 (8895)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77553
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
86537635 |
+ |
CPNE3 |
NNNACTGGCTAATGGTTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
32260276 |
- |
FRY |
NNNACTGCCTGATGGTTCAT |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000004.12 |
4 |
95188880 |
+ |
UNC5C |
NNNATGGGCTAATGGTTCAT |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000009.12 |
9 |
98160839 |
- |
CORO2A |
NNNACTGGCTAACGGGTCAT |
NGG |
2 |
0.2733 |
Tier II |
| 5 |
NC_000005.10 |
5 |
19718715 |
- |
CDH18 |
NNNACTGGCTAATGCATCAT |
NGG |
2 |
0.2479 |
Tier II |
| 6 |
NC_000011.10 |
11 |
22737085 |
+ |
GAS2 |
NNNACTTGCTAATGGTGCAT |
NGG |
2 |
0.0672 |
Tier II |
| 7 |
NC_000019.10 |
19 |
49752527 |
- |
TSKS |
NNNACTGGCTTATGGTTCAC |
NGG |
2 |
0.028 |
Tier II |
| 8 |
NC_000012.12 |
12 |
123138136 |
- |
PITPNM2 |
NNNACTGACTAATGGTTCAT |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000019.10 |
19 |
49752527 |
- |
LOC105372434 |
NNNACTGGCTTATGGTTCAC |
NGG |
2 |
0.028 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
150609646 |
- |
Hmcn1 |
NNNACTGGGTAATGGTTCCT |
NGG |
2 |
0.1281 |
Tier I |
| 2 |
NC_000076.6 |
10 |
81047147 |
+ |
Sgta |
NNNACTGGATAATGGCTCAT |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000074.6 |
8 |
61850991 |
- |
Palld |
NNNACTGGGTAAAGGTTCAT |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000081.6 |
15 |
11156606 |
- |
Adamts12 |
NNNACTGGGTAATGGTTCTT |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000076.6 |
10 |
23802961 |
- |
Slc18b1 |
NNNACTGGCAAATGGGTCAT |
NGG |
2 |
0.2967 |
Tier II |
| 6 |
NC_000085.6 |
19 |
57800989 |
+ |
Atrnl1 |
NNNACTGGATATTGGTTCAT |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000075.6 |
9 |
109156670 |
- |
Fbxw21 |
NNNACTGGTTTATGGTTCAT |
NGG |
2 |
0.2692 |
Tier II |
| 8 |
NC_000068.7 |
2 |
75496657 |
- |
Gm50595 |
NNNTCTGGCTCATGGTTCAT |
NGG |
2 |
0.2545 |
Tier II |
| 9 |
NC_000080.6 |
14 |
39053355 |
- |
Nrg3 |
NNNACTGGCTAATGGTTCAT |
NAA |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
