Construct: sgRNA BRDN0001147460

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCAGCACATGTTAATAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKMT2 (1160)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76596

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 81256949 - CKMT2 NNNAGCACATGTTAATAAAG NGG 0 1.0 Tier I
2 NC_000006.12 6 83138138 - DOP1A NNNAGCACATGTTGATAGAG NGG 2 0.1867 Tier I
3 NC_000011.10 11 94823707 - AMOTL1 NNNAGTACATGTTAATAAAA NGG 2 0.8705 Tier II
4 NC_000002.12 2 207875250 + PLEKHM3 NNNAACACATGTTAATAAAA NGG 2 0.8125 Tier II
5 NC_000013.11 13 24075442 + SPATA13 NNNAACAAATGTTAATAAAG NGG 2 0.5633 Tier II
6 NC_000006.12 6 161468502 - PRKN NNNAGCACATGTCAATAAAT NGG 2 0.5526 Tier II
7 NC_000001.11 1 174229566 + RABGAP1L NNNAGCACAGGTTAAAAAAG NGG 2 0.4545 Tier II
8 NC_000002.12 2 144462117 + ZEB2 NNNAGCACTTGTTAGTAAAG NGG 2 0.39 Tier II
9 NC_000004.12 4 147919059 - ARHGAP10 NNNAGAACATGTTAAGAAAG NGG 2 0.3214 Tier II
10 NC_000001.11 1 3286278 + PRDM16 NNNAGCACGTGTTGATAAAG NGG 2 0.3111 Tier II
11 NC_000001.11 1 3286278 + LOC124903828 NNNAGCACGTGTTGATAAAG NGG 2 0.3111 Tier II
12 NC_000001.11 1 97891787 - DPYD NNNACCACATGTTAAGAAAG NGG 2 0.272 Tier II
13 NC_000004.12 4 21271475 - KCNIP4 NNNAACACATGTTAATAAAG NAG 2 0.2247 Tier II
14 NC_000021.9 21 18094788 + CHODL NNNAGCACATCTTAATAAAC NGG 2 0.1837 Tier II
15 NC_000010.11 10 65992104 - CTNNA3 NNNAACACATGTTAATCAAG NGG 2 0.1529 Tier II
16 NC_000016.10 16 50181722 - TENT4B NNNAGCACATGTACATAAAG NGG 2 0.1484 Tier II
17 NC_000007.14 7 104493219 - LHFPL3 NNNAGCACATTTTAAGAAAG NGG 2 0.1331 Tier II
18 NC_000007.14 7 130488946 - MEST NNNAGCACATGTTAACGAAG NGG 2 0.1176 Tier II
19 NC_000011.10 11 84012698 + DLG2 NNNAGCACATGTGAATAAAC NGG 2 0.1118 Tier II
20 NC_000016.10 16 72963701 - ZFHX3 NNNAGCACATGTTTTTAAAG NGG 2 0.1067 Tier II
21 NC_000002.12 2 164717140 - COBLL1 NNNAGCACATTTTAATAAAG NAG 2 0.0997 Tier II
22 NC_000004.12 4 148029406 - ARHGAP10 NNNAGCACATTTTCATAAAG NGG 2 0.0824 Tier II
23 NC_000002.12 2 166093826 + SCN1A NNNAGCACAAGTTAATAAAG NGA 2 0.0595 Tier II
24 NC_000009.12 9 2809321 + PUM3 NNNAGCACATTTTAATTAAG NGG 2 0.0513 Tier II
25 NC_000001.11 1 46063715 - PIK3R3 NNNAGCACATGTAAATAAAG NGA 2 0.0481 Tier II
26 NC_000001.11 1 46063715 - P3R3URF-PIK3R3 NNNAGCACATGTAAATAAAG NGA 2 0.0481 Tier II
27 NC_000009.12 9 4635534 + SPATA6L NNNAGCACATGTTATTCAAG NGG 2 0.0353 Tier II
28 NC_000003.12 3 18346565 - SATB1 NNNAGCACATGTTAATAAAC NGA 2 0.0298 Tier II
29 NC_000014.9 14 90142785 - KCNK13 NNNAGCACATGTTAATAAAC NTG 2 0.0167 Tier II
30 NC_000015.10 15 92120841 + SLCO3A1 NNNAGCACATGATAATAAAG NGT 2 0.0129 Tier II
31 NC_000005.10 5 81256949 - CKMT2-AS1 NNNAGCACATGTTAATAAAG NGG 0 1.0 Tier III
32 NC_000001.11 1 207180183 + C4BPAP1 NNNAGCAGATGCTAATAAAG NGG 2 0.609 Tier III
33 NC_000001.11 1 207180183 + LOC107985251 NNNAGCAGATGCTAATAAAG NGG 2 0.609 Tier III
34 NC_000009.12 9 126518264 + LOC105376276 NNNAGCAGAAGTTAATAAAG NGG 2 0.551 Tier III
35 NC_000004.12 4 186462453 + F11-AS1 NNNTGCACATGTTAATAAAC NGG 2 0.2727 Tier III
36 NC_000003.12 3 118802168 - LOC105374060 NNNAGCACATGTTAATCAAG NGG 1 0.1765 Tier III
37 NC_000002.12 2 150238693 - LINC01817 NNNAGCACATGTTAAATAAG NGG 2 0.1212 Tier III
38 NC_000002.12 2 150238693 - LINC01818 NNNAGCACATGTTAAATAAG NGG 2 0.1212 Tier III
39 NC_000007.14 7 130488946 - MESTIT1 NNNAGCACATGTTAACGAAG NGG 2 0.1176 Tier III
40 NC_000008.11 8 80179173 - LOC105375920 NNNAGCACATGTTATTAGAG NGG 2 0.08 Tier III
41 NC_000002.12 2 166093826 + SCN1A-AS1 NNNAGCACAAGTTAATAAAG NGA 2 0.0595 Tier III
42 NC_000005.10 5 165229431 - LINC01938 NNNAGCATATGTTAATAAAG NTG 2 0.0341 Tier III
43 NC_000006.12 6 127001204 - LOC105377989 NNNAGCACTTGTTAATAAAG NGT 2 0.0097 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 91859911 + Ckmt2 NNNAGCACATGTTAGTAAAG NGG 1 0.65 Tier I
2 NC_000072.6 6 63737907 - Grid2 NNNAGCATATGATAATAAAG NGG 2 0.7 Tier II
3 NC_000069.6 3 119386627 + Dpyd NNNAACACATGTAAATAAAG NGG 2 0.6 Tier II
4 NC_000086.7 X 161979365 + Nhs NNNAGTACATGTTTATAAAG NGG 2 0.4952 Tier II
5 NC_000084.6 18 25733601 - Celf4 NNNAGCACAGGTTAATAAAA NGG 2 0.4688 Tier II
6 NC_000070.6 4 76537043 + Ptprd NNNAGCACATATTAATAAAG NAG 2 0.2593 Tier II
7 NC_000068.7 2 41434965 - Lrp1b NNNAGGACATGTTGATAAAG NGG 2 0.2333 Tier II
8 NC_000077.6 11 105190525 - Tlk2 NNNAGCACATTGTAATAAAG NGG 2 0.1923 Tier II
9 NC_000068.7 2 113704449 + Fmn1 NNNAGCACTTGTGAATAAAG NGG 2 0.1565 Tier II
10 NC_000069.6 3 51936382 - Maml3 NNNAGCACATGTAAATAACG NGG 2 0.1432 Tier II
11 NC_000081.6 15 101796050 - Krt73 NNNAGCACATGGGAATAAAG NGG 2 0.1304 Tier II
12 NC_000068.7 2 143935701 - Dstn NNNAGCACATTTTAATACAG NGG 2 0.0733 Tier II
13 NC_000079.6 13 69703435 + Ube2ql1 NNNAGCACATTTTAATCAAG NGG 2 0.0679 Tier II
14 NC_000068.7 2 111147181 + A26c3 NNNAGCACATGTTAATAAAT NGA 2 0.0486 Tier II
15 NC_000085.6 19 23433466 + Mamdc2 NNNAGCACATGTTAATGAAG NAG 2 0.0458 Tier II
16 NC_000070.6 4 8827969 - Chd7 NNNAGCACATGTGAATAAAG NGA 2 0.0181 Tier II
17 NC_000077.6 11 101748477 + Dhx8 NNNAGCCCATGTTAATAAAG NTG 2 0.017 Tier II
18 NC_000082.6 16 40797942 + Lsamp NNNAGCACATTTTAATAAAG NTG 2 0.015 Tier II
19 NC_000082.6 16 5202100 - Nagpa NNNAGCACATGTTACTAAAG NTG 2 0.0106 Tier II
20 NC_000072.6 6 129379751 - Clec12b NNNAGCACATGTTAAGAAAG NGT 2 0.0056 Tier II
21 NC_000070.6 4 43158455 + Unc13b NNNAGCACATGTTAATCAAG NGC 2 0.0039 Tier II
22 NC_000068.7 2 33716923 - 9430024E24Rik NNNAGCAGAAGTTAATAAAG NGG 2 0.551 Tier III
23 NC_000068.7 2 63571593 + Gm8541 NNNAGCTCATATTAATAAAG NGG 2 0.4375 Tier III
24 NC_000084.6 18 64223842 + St8sia3os NNNAGCACATGGTAACAAAG NGG 2 0.3333 Tier III
25 NC_000068.7 2 113704449 + 4930533B01Rik NNNAGCACTTGTGAATAAAG NGG 2 0.1565 Tier III
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Other clones with same target sequence:

(none)