Construct: sgRNA BRDN0001147464

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGAGGATAACAGTAACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYRK2 (8445)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77535

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 67657205 + DYRK2 NNNAGGATAACAGTAACAAG NGG 0 1.0 Tier I
2 NC_000011.10 11 134277300 - GLB1L3 NNNAGGACAACAGTGACAAG NGG 2 0.65 Tier I
3 NC_000008.11 8 38129101 + ASH2L NNNAGAATAACAGTAACAAT NGG 2 0.7 Tier II
4 NC_000006.12 6 144742216 - UTRN NNNAGGATGACAATAACAAG NGG 2 0.6154 Tier II
5 NC_000014.9 14 57751448 - SLC35F4 NNNTGGATAACAGTAACAAA NGG 2 0.5966 Tier II
6 NC_000014.9 14 72378053 + RGS6 NNNTGGATATCAGTAACAAG NGG 2 0.5615 Tier II
7 NC_000005.10 5 53980700 + ARL15 NNNAGGAAAGCAGTAACAAG NGG 2 0.4444 Tier II
8 NC_000007.14 7 87584788 - ABCB1 NNNAGGAAAACAGTAACAGG NGG 2 0.3 Tier II
9 NC_000006.12 6 42050962 - TAF8 NNNATGAAAACAGTAACAAG NGG 2 0.24 Tier II
10 NC_000002.12 2 70513618 + TGFA NNNAGGATAACAGTAGCAAG NGG 1 0.1923 Tier II
11 NC_000002.12 2 20331382 - PUM2 NNNAGGATAACAGTAACCAA NGG 2 0.1786 Tier II
12 NC_000005.10 5 78178176 + AP3B1 NNNAGGATAACAGTAACTAG NAG 2 0.1296 Tier II
13 NC_000003.12 3 114318415 + ZBTB20 NNNATGATAACAGTAACAGG NGG 2 0.1125 Tier II
14 NC_000008.11 8 139873353 + TRAPPC9 NNNAGGATAACAGTAATAAG NGA 2 0.0324 Tier II
15 NC_000007.14 7 40471524 + SUGCT NNNGGGATAACAGTAACAAG NTG 2 0.0244 Tier II
16 NC_000006.12 6 166474306 + RPS6KA2 NNNAGGAAAACAGTAACAAG NGC 2 0.0178 Tier II
17 NC_000001.11 1 89258278 + GBP5 NNNAGGTTAACAGTAACAAG NTG 2 0.017 Tier II
18 NC_000023.11 X 116446261 + SLC6A14 NNNAGGATAACAGTAACAAG NGT 1 0.0161 Tier II
19 NC_000021.9 21 14503604 + SAMSN1 NNNAGGATAAGAGTAAGAAG NGG 2 0.0147 Tier II
20 NC_000015.10 15 71404735 + THSD4 NNNAGGATAACAGCAACAAG NTG 2 0.0111 Tier II
21 NC_000015.10 15 60737957 + RORA NNNAGGATAACAGTTACAAG NGC 2 0.0044 Tier II
22 NC_000011.10 11 88306601 + CTSC NNNAGGAGAACAGGAACAAG NGG 2 0.0 Tier II
23 NC_000012.12 12 41431024 + PDZRN4 NNNAGGAGAACAGTACCAAG NGG 2 0.0 Tier II
24 NC_000012.12 12 8085450 - NECAP1 NNNAGGATAACAGGAAAAAG NGG 2 0.0 Tier II
25 NC_000003.12 3 157227666 + LOC101928236 NNNAGGACAATAGTAACAAG NGG 2 0.3077 Tier III
26 NC_000006.12 6 22702013 + LINC03005 NNNAGGATAAGAGTTACAAG NGG 2 0.05 Tier III
27 NC_000007.14 7 26586232 + LOC101928077 NNNAGGAAAACAGTAAGAAG NGG 2 0.0471 Tier III
28 NC_000022.11 22 34781446 - LINC02885 NNNAGGATAAAAGTAACAAG NTG 2 0.0292 Tier III
29 NC_000001.11 1 89258278 + LOC100421401 NNNAGGTTAACAGTAACAAG NTG 2 0.017 Tier III
30 NC_000021.9 21 14503604 + LOC124905053 NNNAGGATAAGAGTAAGAAG NGG 2 0.0147 Tier III
31 NC_000001.11 1 70836647 - LOC102724572 NNNAGGATAACAGTACCAAG NTG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 11802739 - Fignl1 NNNAGGCTGACAGTAACAAG NGG 2 0.2917 Tier I
2 NC_000076.6 10 118861061 - Dyrk2 NNNAGGATAATAGTAACAAA NGG 2 0.2885 Tier I
3 NC_000075.6 9 100825883 - Stag1 NNNAGGATAAAAGTAACAAA NGG 2 0.7031 Tier II
4 NC_000067.6 1 124680272 + Dpp10 NNNTGAATAACAGTAACAAG NGG 2 0.6364 Tier II
5 NC_000074.6 8 72902278 - Large1 NNNTGAATAACAGTAACAAG NGG 2 0.6364 Tier II
6 NC_000067.6 1 165861660 + Cd247 NNNAGGGGAACAGTAACAAG NGG 2 0.5176 Tier II
7 NC_000067.6 1 180235994 + Psen2 NNNTGGATAACAGAAACAAG NGG 2 0.3939 Tier II
8 NC_000075.6 9 7011388 - Dync2h1 NNNAGGATAAAAGTAATAAG NGG 2 0.35 Tier II
9 NC_000074.6 8 45785366 - Sorbs2 NNNAGGTTGACAGTAACAAG NGG 2 0.2917 Tier II
10 NC_000078.6 12 100452514 - Ttc7b NNNAGGAGAACAGTAACAGG NGG 2 0.275 Tier II
11 NC_000078.6 12 34412432 + Hdac9 NNNAGGATAACAATAACAAG NAG 2 0.2393 Tier II
12 NC_000069.6 3 126781898 + Camk2d NNNATCATAACAGTAACAAG NGG 2 0.2045 Tier II
13 NC_000067.6 1 124378389 + Dpp10 NNNAGGATAACAGATACAAG NGG 2 0.1238 Tier II
14 NC_000068.7 2 181111952 + Kcnq2 NNNAGGATAGCAGTAACCAG NGG 2 0.1058 Tier II
15 NC_000073.6 7 111614765 - Galnt18 NNNTGGATAACAGTAAGAAG NGG 2 0.0374 Tier II
16 NC_000067.6 1 125990394 - Nckap5 NNNAGGATAACAGGAAAAAG NGG 2 0.0 Tier II
17 NC_000069.6 3 67228770 + Rsrc1 NNNAGGATAACAGGACCAAG NGG 2 0.0 Tier II
18 NC_000082.6 16 70994649 - Gm29823 NNNAGGAAAGCAGTAACAAG NGG 2 0.4444 Tier III
19 NC_000074.6 8 45785366 - Sorbs2os NNNAGGTTGACAGTAACAAG NGG 2 0.2917 Tier III
20 NC_000071.6 5 141152695 - Gm30866 NNNAGGATAACAGAAATAAG NGG 2 0.2889 Tier III
21 NC_000070.6 4 64177147 - Gm31115 NNNGGGATAAGAGTAACAAG NGG 2 0.1563 Tier III
22 NC_000081.6 15 36705319 - Gm41296 NNNAGGATTACAGTATCAAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)