Construct: sgRNA BRDN0001147469
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCTGCAACACTACACCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB2 (2048)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77546
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
22896468 |
+ |
EPHB2 |
NNNCTGCAACACTACACCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
62552597 |
- |
TLN2 |
NNNCTTCAGCACTACACCAG |
NGG |
2 |
0.4444 |
Tier II |
| 3 |
NC_000003.12 |
3 |
53626174 |
- |
CACNA1D |
NNNCTGCCACACTACACCAG |
NGG |
1 |
0.4286 |
Tier II |
| 4 |
NC_000013.11 |
13 |
27550161 |
- |
LNX2 |
NNNCTCCCACACTACACCAG |
NGG |
2 |
0.2922 |
Tier II |
| 5 |
NC_000015.10 |
15 |
36807394 |
+ |
CDIN1 |
NNNCTGCAACACTACACCAA |
NAG |
2 |
0.2431 |
Tier II |
| 6 |
NC_000004.12 |
4 |
7736294 |
- |
SORCS2 |
NNNCTGCAACACTTCACCAC |
NGG |
2 |
0.2286 |
Tier II |
| 7 |
NC_000022.11 |
22 |
28003840 |
+ |
TTC28 |
NNNCTCCAACACTCCACCAG |
NGG |
2 |
0.1461 |
Tier II |
| 8 |
NC_000014.9 |
14 |
102430658 |
- |
TECPR2 |
NNNCTGCAACACGACACCAC |
NGG |
2 |
0.1118 |
Tier II |
| 9 |
NC_000015.10 |
15 |
79386004 |
+ |
TMED3 |
NNNCTACAACACTAGACCAG |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000012.12 |
12 |
120579132 |
+ |
POP5 |
NNNCTGCAACACTTCACCAG |
NGA |
2 |
0.037 |
Tier II |
| 11 |
NC_000017.11 |
17 |
80666728 |
- |
RPTOR |
NNNCTGCACCACTACAGCAG |
NGG |
2 |
0.0336 |
Tier II |
| 12 |
NC_000009.12 |
9 |
114481713 |
+ |
WHRN |
NNNCTGCAACACTACACCAC |
NGC |
2 |
0.0095 |
Tier II |
| 13 |
NC_000011.10 |
11 |
36070804 |
+ |
LDLRAD3 |
NNNCTGCAACACTGCCCCAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000015.10 |
15 |
36807394 |
+ |
CSNK1A1P1 |
NNNCTGCAACACTACACCAA |
NAG |
2 |
0.2431 |
Tier III |
| 15 |
NC_000011.10 |
11 |
36070804 |
+ |
LOC101928510 |
NNNCTGCAACACTGCCCCAG |
NGG |
2 |
0.0 |
Tier III |
| 16 |
NC_000004.12 |
4 |
171609589 |
+ |
LINC02174 |
NNNCTGCAACATTACCCCAG |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000011.10 |
11 |
116436398 |
+ |
LOC107987166 |
NNNCTGGAACACTACCCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
136672454 |
- |
Ephb2 |
NNNCTACAACACTACACCAG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
16955734 |
+ |
Csmd1 |
NNNCTGCAACATTACACCAG |
NGG |
1 |
0.5385 |
Tier II |
| 3 |
NC_000073.6 |
7 |
44954741 |
+ |
Tsks |
NNNCTGCAACATAACACCAG |
NGG |
2 |
0.3728 |
Tier II |
| 4 |
NC_000082.6 |
16 |
11380820 |
- |
Snx29 |
NNNCTTCAAGACTACACCAG |
NGG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000068.7 |
2 |
115775856 |
+ |
BC052040 |
NNNCTGCAACACTACACCAA |
NAG |
2 |
0.2431 |
Tier II |
| 6 |
NC_000083.6 |
17 |
55966779 |
+ |
Yju2 |
NNNCTGCCACACTGCACCAG |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000073.6 |
7 |
55142868 |
+ |
Luzp2 |
NNNCTGCAACACTACGCCAG |
NGG |
1 |
0.1923 |
Tier II |
| 8 |
NC_000073.6 |
7 |
30235936 |
- |
Ovol3 |
NNNCTGCCACCCTACACCAG |
NGG |
2 |
0.1714 |
Tier II |
| 9 |
NC_000085.6 |
19 |
31745811 |
+ |
Prkg1 |
NNNCTGCATCACTAGACCAG |
NGG |
2 |
0.03 |
Tier II |
| 10 |
NC_000075.6 |
9 |
83139270 |
+ |
Hmgn3 |
NNNCTGCAACACAACACCAG |
NTG |
2 |
0.027 |
Tier II |
| 11 |
NC_000077.6 |
11 |
121366459 |
- |
Rab40b |
NNNCTGCAACACTACTCCTG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000068.7 |
2 |
115775856 |
+ |
Gm52524 |
NNNCTGCAACACTACACCAA |
NAG |
2 |
0.2431 |
Tier III |
| 13 |
NC_000069.6 |
3 |
156550831 |
- |
4930570G19Rik |
NNNCTGCAAGACTACAGCAG |
NGG |
2 |
0.0229 |
Tier III |
Other clones with same target sequence:
(none)
