Construct: sgRNA BRDN0001147476
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGTCCGCAAGAACCAAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TIE1 (7075)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76688
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
43309445 |
- |
TIE1 |
NNNTCCGCAAGAACCAAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
168850184 |
+ |
PALLD |
NNNTCTGCCAGAACCAAGCG |
NGG |
2 |
0.5306 |
Tier II |
| 3 |
NC_000019.10 |
19 |
45427846 |
+ |
ERCC1 |
NNNTCCGCCAGAACCCAGCG |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000004.12 |
4 |
168850184 |
+ |
LOC102723331 |
NNNTCTGCCAGAACCAAGCG |
NGG |
2 |
0.5306 |
Tier III |
| 5 |
NC_000005.10 |
5 |
135270208 |
+ |
PITX1-AS1 |
NNNTCCCCAAGAGCCAAGCG |
NGG |
2 |
0.4484 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
100412525 |
- |
Slc11a2 |
NNNCCCACAAGAACCAAGCG |
NGG |
2 |
0.6471 |
Tier II |
| 2 |
NC_000067.6 |
1 |
89739976 |
+ |
Agap1 |
NNNTCCTCAAGAACCAAGCC |
NGG |
2 |
0.2449 |
Tier II |
| 3 |
NC_000072.6 |
6 |
118747233 |
+ |
Cacna1c |
NNNTCCGCAAGTACCAAGAG |
NGG |
2 |
0.1429 |
Tier II |
| 4 |
NC_000084.6 |
18 |
74200506 |
+ |
Ska1 |
NNNTCAGCAAGAACCAAGGG |
NGG |
2 |
0.1161 |
Tier II |
| 5 |
NC_000076.6 |
10 |
39108560 |
+ |
Lama4 |
NNNTCCGCATGAACCAAGGG |
NGG |
2 |
0.1103 |
Tier II |
Other clones with same target sequence:
(none)
