Construct: sgRNA BRDN0001147480

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGACTTCTGCATACAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME1-NME2 (654364)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75886

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 51161264 + NME1 NNNGACTTCTGCATACAAGT NGG 0 1.0 Tier I
2 NC_000017.11 17 51161264 + NME1-NME2 NNNGACTTCTGCATACAAGT NGG 0 1.0 Tier I
3 NC_000017.11 17 51170041 + NME2 NNNGACTTCTGCATTCAGGT NGG 2 0.08 Tier I
4 NC_000017.11 17 51170041 + NME1-NME2 NNNGACTTCTGCATTCAGGT NGG 2 0.08 Tier I
5 NC_000013.11 13 32124839 - FRY NNNGACTTCTGCATACAGGT NTG 2 0.0156 Tier I
6 NC_000009.12 9 16632815 - BNC2 NNNGACTTTTGCATAAAAGT NGG 2 0.875 Tier II
7 NC_000016.10 16 2771182 - SRRM2 NNNAACTTCTGGATACAAGT NGG 2 0.4 Tier II
8 NC_000007.14 7 114143202 - FOXP2 NNNGATTTCTCCATACAAGT NGG 2 0.398 Tier II
9 NC_000006.12 6 24970388 + RIPOR2 NNNGAATTCTGCATACAGGT NGG 2 0.3714 Tier II
10 NC_000018.10 18 75266508 - TSHZ1 NNNTACTTCTACATACAAGT NGG 2 0.3636 Tier II
11 NC_000019.10 19 40088289 - ZNF780A NNNGACTTCTGCATACATTT NGG 2 0.3333 Tier II
12 NC_000016.10 16 10163469 + GRIN2A NNNAACTTCTGCATACACGT NGG 2 0.1714 Tier II
13 NC_000003.12 3 134703898 - CEP63 NNNGACTTCAGCATTCAAGT NGG 2 0.1714 Tier II
14 NC_000019.10 19 48102007 + PLA2G4C NNNGACTTCTGCATCCAACT NGG 2 0.1223 Tier II
15 NC_000015.10 15 60619661 + RORA NNNGAGTTCTGCCTACAAGT NGG 2 0.1053 Tier II
16 NC_000004.12 4 99539012 - C4orf17 NNNGACTTCTGCAGAAAAGT NGG 2 0.0 Tier II
17 NC_000009.12 9 13474067 - LOC105375977 NNNCACTTCTGCATACAAAT NGG 2 0.3782 Tier III
18 NC_000001.11 1 40505396 + EXO5-DT NNNGACTTTTGCATACAGGT NGG 2 0.35 Tier III
19 NC_000012.12 12 102510022 - LINC02456 NNNGAGTTCTGTATACAAGT NGG 2 0.2692 Tier III
20 NC_000005.10 5 151638009 - LOC105378231 NNNGACTTCCGCATATAAGT NGG 2 0.1641 Tier III
21 NT_187420.1 238557 + LOC101930589 NNNGTCTTCTGCTTACAAGT NGG 2 0.1091 Tier III
22 NC_000015.10 15 60619661 + RORA-AS1 NNNGAGTTCTGCCTACAAGT NGG 2 0.1053 Tier III
23 NC_000017.11 17 31055085 - LOC646030 NNNGACTTCTGCAAAGAAGT NGG 2 0.0952 Tier III
24 NC_000009.12 9 72275753 + LOC100289320 NNNGACTTCTGCATTCAGGT NGG 2 0.08 Tier III
25 NC_000006.12 6 109484734 + ZBTB24-DT NNNGACTTCTGCATACCAGC NGG 2 0.016 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 93960677 - Nme1 NNNGACTTCTGCATCCAAGT NGG 1 0.2727 Tier I
2 NC_000077.6 11 93951909 - Nme2 NNNGATTTCTGCATTCAAGT NGG 2 0.1857 Tier I
3 NC_000073.6 7 132078179 - Cpxm2 NNNAACTTCTGCATACAAGT NGG 1 0.9 Tier II
4 NC_000075.6 9 95201113 + Slc9a9 NNNGACCACTGCATACAAGT NGG 2 0.55 Tier II
5 NC_000080.6 14 12293964 - 3830406C13Rik NNNGACTACTGCATACAGGT NGG 2 0.32 Tier II
6 NC_000073.6 7 16785308 + Slc1a5 NNNGACTCCTGCACACAAGT NGG 2 0.2857 Tier II
7 NC_000070.6 4 70233070 + Cdk5rap2 NNNGACTTCTCCATGCAAGT NGG 2 0.2786 Tier II
8 NC_000086.7 X 137638800 + Il1rapl2 NNNGACCTCTGCATCCAAGT NGG 2 0.1875 Tier II
9 NC_000079.6 13 108685605 + Pde4d NNNGATTTCTGCATTCAAGT NGG 2 0.1857 Tier II
10 NC_000077.6 11 5380583 - Znrf3 NNNTACTTCTGCATACATGT NGG 2 0.1818 Tier II
11 NC_000070.6 4 59341431 - Susd1 NNNGACTTCTTCATACAACT NGG 2 0.1724 Tier II
12 NC_000080.6 14 75219155 + Lcp1 NNNGATTTCTGCATACAAGG NGG 2 0.1639 Tier II
13 NC_000075.6 9 28611937 + Opcml NNNGACTTCTGCCAACAAGT NGG 2 0.1303 Tier II
14 NC_000069.6 3 117835832 + Snx7 NNNGACTTCTGCTTATAAGT NGG 2 0.0923 Tier II
15 NC_000072.6 6 85455147 + Cct7 NNNGACTTCTGCATACAATT NGA 2 0.0463 Tier II
16 NC_000069.6 3 144491984 + Hs2st1 NNNGAATTCTGCATACAAGT NGT 2 0.015 Tier II
17 NC_000069.6 3 144491984 + Gm43707 NNNGAATTCTGCATACAAGT NGT 2 0.015 Tier II
18 NC_000081.6 15 27753067 - Trio NNNGACTTCTGCTGACAAGT NGG 2 0.0 Tier II
19 NC_000081.6 15 95335016 - Nell2 NNNGAGTTCTGCAGACAAGT NGG 2 0.0 Tier II
20 NC_000078.6 12 58549944 + Gm30687 NNNGACTTCTGCATAAAATT NGG 2 0.6667 Tier III
21 NC_000072.6 6 54385808 + 9130019P16Rik NNNGACTTCTACATACAACT NGG 2 0.4483 Tier III
22 NC_000073.6 7 119301378 - Gm4083 NNNGATTTCTGCATCCAAGT NGG 2 0.2532 Tier III
23 NC_000071.6 5 149660892 - Gm5566 NNNGATTTCTGCATTCAAGT NGG 2 0.1857 Tier III
24 NC_000074.6 8 70010671 - Gm7730 NNNGATTTCTGCATTCAAGT NGG 2 0.1857 Tier III
25 NC_000076.6 10 84309218 + Gm5425 NNNGATTTCTGCATTCAAGT NGG 2 0.1857 Tier III
26 NC_000071.6 5 144510460 + Gm33765 NNNGACTTCTGCATACATGT NAG 2 0.1296 Tier III
27 NC_000072.6 6 49656957 - Gm31226 NNNGACTTCTGCAGATAAGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)