Construct: sgRNA BRDN0001147481
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCGAACGTGACACAGGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FES (2242)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76508
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
90889538 |
- |
FES |
NNNCGAACGTGACACAGGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
36419868 |
+ |
RNF38 |
NNNTGAACCTGACACAGGGT |
NGG |
2 |
0.4308 |
Tier II |
| 3 |
NC_000017.11 |
17 |
75632802 |
+ |
RECQL5 |
NNNGGAACGTGGCACAGGGT |
NGG |
2 |
0.3611 |
Tier II |
| 4 |
NC_000002.12 |
2 |
218878766 |
- |
WNT10A |
NNNGGAACGTGACACAGGGA |
NGG |
2 |
0.2813 |
Tier II |
| 5 |
NC_000019.10 |
19 |
50399696 |
+ |
POLD1 |
NNNCGAAGGTGACACAGGGT |
NAG |
2 |
0.1667 |
Tier II |
| 6 |
NC_000017.11 |
17 |
64089362 |
+ |
ERN1 |
NNNGGAACGTGACACAGGGT |
NAG |
2 |
0.1296 |
Tier II |
| 7 |
NC_000006.12 |
6 |
44040094 |
- |
POLR1C |
NNNTGAACGTGACACAGGGC |
NGG |
2 |
0.0727 |
Tier II |
| 8 |
NC_000006.12 |
6 |
44040094 |
- |
SCIRT |
NNNTGAACGTGACACAGGGC |
NGG |
2 |
0.0727 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
70320201 |
+ |
Gbe1 |
NNNCGAACAAGACACAGGGT |
NGG |
2 |
0.551 |
Tier II |
| 2 |
NC_000081.6 |
15 |
78007835 |
+ |
Cacng2 |
NNNGGAACGTGACACAGGGT |
NGG |
1 |
0.5 |
Tier II |
| 3 |
NC_000071.6 |
5 |
24322899 |
- |
Kcnh2 |
NNNGGAAAGTGACACAGGGT |
NGG |
2 |
0.325 |
Tier II |
| 4 |
NC_000083.6 |
17 |
28575517 |
+ |
Lhfpl5 |
NNNGGAACGTGACACACGGT |
NGG |
2 |
0.1176 |
Tier II |
| 5 |
NC_000082.6 |
16 |
33904419 |
+ |
Itgb5 |
NNNCTAACGTGACACAGGGT |
NTG |
2 |
0.0117 |
Tier II |
| 6 |
NC_000071.6 |
5 |
24322899 |
- |
Gm15589 |
NNNGGAAAGTGACACAGGGT |
NGG |
2 |
0.325 |
Tier III |
Other clones with same target sequence:
(none)
