Construct: sgRNA BRDN0001147486
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGCACAGCTTCAAGCCAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- HSPB8 (26353)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000012.12 | 12 | 119179632 | + | HSPB8 | NNNGCACAGCTTCAAGCCAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000016.10 | 16 | 29697123 | + | QPRT | NNNGGACAACTTCAAGCCAG | NGG | 2 | 0.3857 | Tier I |
3 | NC_000019.10 | 19 | 2876115 | + | ZNF556 | NNNGCTCAGCTTAAAGCCAG | NGG | 2 | 0.2747 | Tier I |
4 | NC_000002.12 | 2 | 186722846 | - | FAM171B | NNNGCACAACTCCAAGCCAG | NGG | 2 | 0.609 | Tier II |
5 | NC_000006.12 | 6 | 107755520 | - | SCML4 | NNNGCAAAGCTTCTAGCCAG | NGG | 2 | 0.4 | Tier II |
6 | NC_000013.11 | 13 | 70016776 | + | KLHL1 | NNNGCTCACCTTCAAGCCAG | NGG | 2 | 0.3846 | Tier II |
7 | NC_000002.12 | 2 | 10830206 | + | PDIA6 | NNNGCGCAGCTTCAAGACAG | NGG | 2 | 0.3333 | Tier II |
8 | NC_000001.11 | 1 | 153643452 | + | CHTOP | NNNGCACAGCTTCAAGTCAT | NGG | 2 | 0.3267 | Tier II |
9 | NC_000012.12 | 12 | 76470712 | + | OSBPL8 | NNNGCACAGCATCAAGCCAC | NGG | 2 | 0.3214 | Tier II |
10 | NC_000016.10 | 16 | 28580499 | - | SGF29 | NNNGGACAGCTGCAAGCCAG | NGG | 2 | 0.3 | Tier II |
11 | NC_000015.10 | 15 | 29089627 | - | APBA2 | NNNCCACAGCTTCAAGCCTG | NGG | 2 | 0.2851 | Tier II |
12 | NC_000022.11 | 22 | 24370341 | + | SPECC1L | NNNTCTCAGCTTCAAGCCAG | NGG | 2 | 0.2597 | Tier II |
13 | NC_000023.11 | X | 72346447 | + | HDAC8 | NNNGCACTGCTTCAAGCCTG | NGG | 2 | 0.2308 | Tier II |
14 | NC_000015.10 | 15 | 40228850 | - | BUB1B-PAK6 | NNNGCCCAGCTGCAAGCCAG | NGG | 2 | 0.2273 | Tier II |
15 | NC_000002.12 | 2 | 230878355 | - | ITM2C | NNNGCCCAGCTTCAAGACAG | NGG | 2 | 0.2121 | Tier II |
16 | NC_000004.12 | 4 | 52903487 | - | SCFD2 | NNNGCACAGCTTCAAGTCAC | NGG | 2 | 0.2 | Tier II |
17 | NC_000008.11 | 8 | 139865050 | + | TRAPPC9 | NNNGCACCGGTTCAAGCCAG | NGG | 2 | 0.1667 | Tier II |
18 | NC_000023.11 | X | 152899532 | - | ZNF185 | NNNGCACAGCATCCAGCCAG | NGG | 2 | 0.1607 | Tier II |
19 | NC_000002.12 | 2 | 120276200 | - | RALB | NNNGCACAGCTGCATGCCAG | NGG | 2 | 0.1 | Tier II |
20 | NC_000002.12 | 2 | 127566090 | + | MYO7B | NNNGCACAGCCTGAAGCCAG | NGG | 2 | 0.0909 | Tier II |
21 | NC_000002.12 | 2 | 127659875 | - | LIMS2 | NNNGCACAGCTGGAAGCCAG | NGG | 2 | 0.0682 | Tier II |
22 | NC_000015.10 | 15 | 45659608 | - | SQOR | NNNGCACAGCTACAAGCCAG | NGA | 2 | 0.0556 | Tier II |
23 | NC_000009.12 | 9 | 130317212 | - | HMCN2 | NNNTCACAGCTTGAAGCCAG | NGG | 2 | 0.0496 | Tier II |
24 | NC_000002.12 | 2 | 21026214 | + | APOB | NNNGCACAGCTTCAAGACAG | NGA | 2 | 0.0324 | Tier II |
25 | NC_000020.11 | 20 | 38234921 | - | KIAA1755 | NNNGCACAGCTTCAAGCCAC | NGT | 2 | 0.0069 | Tier II |
26 | NC_000018.10 | 18 | 55358022 | + | TCF4 | NNNGCACAGCTTCAAGCCAG | NCT | 2 | 0.0 | Tier II |
27 | NC_000005.10 | 5 | 72808942 | + | TNPO1-DT | NNNGCAAAGTTTCAAGCCAG | NGG | 2 | 0.7059 | Tier III |
28 | NC_000013.11 | 13 | 30889197 | - | TEX26-AS1 | NNNGCACAGCCTCAAGCCAA | NGG | 2 | 0.625 | Tier III |
29 | NC_000005.10 | 5 | 152220079 | + | LINC01933 | NNNGCACAGCTCCAGGCCAG | NGG | 2 | 0.6158 | Tier III |
30 | NC_000023.11 | X | 39227110 | + | LOC105373175 | NNNGGACAGCTCCAAGCCAG | NGG | 2 | 0.5684 | Tier III |
31 | NC_000002.12 | 2 | 119744399 | + | LOC101927764 | NNNGAAGAGCTTCAAGCCAG | NGG | 2 | 0.2689 | Tier III |
32 | NC_000022.11 | 22 | 24370341 | + | SPECC1L-ADORA2A | NNNTCTCAGCTTCAAGCCAG | NGG | 2 | 0.2597 | Tier III |
33 | NC_000010.11 | 10 | 65779455 | + | LOC105378339 | NNNCCCCAGCTTCAAGCCAG | NGG | 2 | 0.2406 | Tier III |
34 | NC_000015.10 | 15 | 40228850 | - | LOC107984763 | NNNGCCCAGCTGCAAGCCAG | NGG | 2 | 0.2273 | Tier III |
35 | NC_000008.11 | 8 | 139865050 | + | LOC107986981 | NNNGCACCGGTTCAAGCCAG | NGG | 2 | 0.1667 | Tier III |
36 | NC_000002.12 | 2 | 127566090 | + | LOC105373609 | NNNGCACAGCCTGAAGCCAG | NGG | 2 | 0.0909 | Tier III |
37 | NC_000006.12 | 6 | 14992878 | + | LOC105374945 | NNNGGACAGCTTGAAGCCAG | NGG | 2 | 0.0818 | Tier III |
38 | NC_000019.10 | 19 | 16038560 | - | LINC00905 | NNNGCACAGCTGGAAGCCAG | NGG | 2 | 0.0682 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 88889802 | - | Mcm2 | NNNGGACAGCTGCAAGCCAG | NGG | 2 | 0.3 | Tier I |
2 | NC_000071.6 | 5 | 116422161 | - | Hspb8 | NNNGCACAGCTTCAAGCCGG | NAG | 2 | 0.0972 | Tier I |
3 | NC_000069.6 | 3 | 153706749 | - | St6galnac3 | NNNACACAGATTCAAGCCAG | NGG | 2 | 0.78 | Tier II |
4 | NC_000081.6 | 15 | 30681145 | + | Ctnnd2 | NNNGCACAGCTTCAAGCCAT | NGG | 1 | 0.7 | Tier II |
5 | NC_000073.6 | 7 | 118889207 | - | Iqck | NNNACAAAGCTTCAAGCCAG | NGG | 2 | 0.675 | Tier II |
6 | NC_000068.7 | 2 | 68971223 | - | Cers6 | NNNGGAAAGCTTCAAGCCAG | NGG | 2 | 0.45 | Tier II |
7 | NC_000078.6 | 12 | 89362632 | - | Nrxn3 | NNNGCATAGCTTCAAGCAAG | NGG | 2 | 0.4375 | Tier II |
8 | NC_000067.6 | 1 | 165835541 | - | Cd247 | NNNGCACAGGTTCAAACCAG | NGG | 2 | 0.3889 | Tier II |
9 | NC_000071.6 | 5 | 29271947 | - | Lmbr1 | NNNGCACAGGTTCAAGCCAG | NGG | 1 | 0.3889 | Tier II |
10 | NC_000069.6 | 3 | 90502030 | - | Chtop | NNNGCACAGCTTCAAGTCAT | NGG | 2 | 0.3267 | Tier II |
11 | NC_000086.7 | X | 20144562 | - | Slc9a7 | NNNGTACAGCTTCAAGTCAG | NGG | 2 | 0.297 | Tier II |
12 | NC_000068.7 | 2 | 109105998 | - | Mettl15 | NNNGCTCAGGTTCAAGCCAG | NGG | 2 | 0.2778 | Tier II |
13 | NC_000082.6 | 16 | 40845030 | + | Lsamp | NNNGCACAAGTTCAAGCCAG | NGG | 2 | 0.25 | Tier II |
14 | NC_000067.6 | 1 | 127339210 | - | Mgat5 | NNNGCAGAGCTGCAAGCCAG | NGG | 2 | 0.2353 | Tier II |
15 | NC_000069.6 | 3 | 76540231 | - | Fstl5 | NNNGCACAGGTTCAAGCCAG | NAG | 2 | 0.1008 | Tier II |
16 | NC_000072.6 | 6 | 13655959 | - | Bmt2 | NNNGCACAGGTTCAAGCCAG | NAG | 2 | 0.1008 | Tier II |
17 | NC_000081.6 | 15 | 5113536 | + | Card6 | NNNGCACAGGTTCAAGCCAG | NAG | 2 | 0.1008 | Tier II |
18 | NC_000084.6 | 18 | 62558993 | - | Spink10 | NNNGCACAGGTTCAAGCCAG | NAG | 2 | 0.1008 | Tier II |
19 | NC_000067.6 | 1 | 135265118 | - | Rnpep | NNNGCACAGCTTCATGTCAG | NGG | 2 | 0.0933 | Tier II |
20 | NC_000080.6 | 14 | 99197665 | - | Pibf1 | NNNGCACAGGTTCCAGCCAG | NGG | 2 | 0.0833 | Tier II |
21 | NC_000080.6 | 14 | 64300231 | - | Msra | NNNGCACAGCTTGAAGCCAC | NGG | 2 | 0.0584 | Tier II |
22 | NC_000081.6 | 15 | 21496231 | - | Cdh12 | NNNGCACAGCTTTAAGCCAG | NGA | 2 | 0.0486 | Tier II |
23 | NC_000072.6 | 6 | 86953968 | - | Aak1 | NNNGCACAGGTTCAAGCCAG | NCG | 2 | 0.0417 | Tier II |
24 | NC_000074.6 | 8 | 86650982 | - | Lonp2 | NNNGCACAGGTTCAAGCCAG | NCG | 2 | 0.0417 | Tier II |
25 | NC_000083.6 | 17 | 68329968 | - | L3mbtl4 | NNNGCACAGGTTCAAGCCAG | NCG | 2 | 0.0417 | Tier II |
26 | NC_000074.6 | 8 | 65796245 | - | Marchf1 | NNNGCACAGATTCAAGCCAG | NTG | 2 | 0.0338 | Tier II |
27 | NC_000068.7 | 2 | 68438156 | - | Stk39 | NNNGCAGAGCTTCAAGCCAG | NGA | 2 | 0.0327 | Tier II |
28 | NC_000080.6 | 14 | 10059548 | - | Fhit | NNNGCTCAGCTTCAAGCCAG | NTG | 2 | 0.0278 | Tier II |
29 | NC_000083.6 | 17 | 75505902 | - | Rasgrp3 | NNNGCACAGGTTCAAGGCAG | NGG | 2 | 0.0229 | Tier II |
30 | NC_000085.6 | 19 | 52983813 | + | Xpnpep1 | NNNGCACTGCTTCAAGCCAG | NTG | 2 | 0.0167 | Tier II |
31 | NC_000079.6 | 13 | 106931140 | + | Ipo11 | NNNGCACAGCTTCAAGCCAG | NGT | 1 | 0.0161 | Tier II |
32 | NC_000067.6 | 1 | 53325341 | + | Osgepl1 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
33 | NC_000067.6 | 1 | 187106580 | + | Spata17 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
34 | NC_000070.6 | 4 | 98618646 | - | Patj | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
35 | NC_000072.6 | 6 | 13656636 | - | Bmt2 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
36 | NC_000072.6 | 6 | 28270051 | - | Zfp800 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
37 | NC_000075.6 | 9 | 56314682 | + | Peak1 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
38 | NC_000075.6 | 9 | 67881133 | - | Vps13c | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
39 | NC_000078.6 | 12 | 83870233 | + | Numb | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
40 | NC_000081.6 | 15 | 53819550 | + | Samd12 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
41 | NC_000081.6 | 15 | 94825660 | - | Tmem117 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
42 | NC_000082.6 | 16 | 31928452 | - | Pigz | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
43 | NC_000083.6 | 17 | 66719346 | + | Ptprm | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
44 | NC_000086.7 | X | 36944710 | + | Septin6 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier II |
45 | NC_000075.6 | 9 | 15304648 | + | 4931406C07Rik | NNNGCACAGCTTCCAGCCAG | NTG | 2 | 0.0083 | Tier II |
46 | NC_000069.6 | 3 | 87489301 | + | Fcrl5 | NNNGCACAGCTTCAAGACAG | NGT | 2 | 0.0075 | Tier II |
47 | NC_000080.6 | 14 | 75088242 | - | Lrrc63 | NNNGCACAGGTTCAAGCCAG | NGT | 2 | 0.0063 | Tier II |
48 | NC_000085.6 | 19 | 5230228 | - | Pacs1 | NNNGCACAGGTTCAAGCCAG | NGT | 2 | 0.0063 | Tier II |
49 | NC_000067.6 | 1 | 51064800 | + | Tmeff2 | NNNTCACAGCTTCAAGCCAG | NGT | 2 | 0.0059 | Tier II |
50 | NC_000079.6 | 13 | 15485706 | + | Gli3 | NNNGCACAGCTACAACCCAG | NGG | 2 | 0.0 | Tier II |
51 | NC_000072.6 | 6 | 48836405 | + | Tmem176b | NNNGCACAGCTCCAACCCAG | NGG | 2 | 0.0 | Tier II |
52 | NC_000068.7 | 2 | 49720142 | - | Kif5c | NNNGCACAGCTTCAACCCAG | NGA | 2 | 0.0 | Tier II |
53 | NC_000077.6 | 11 | 5626517 | + | Ankrd36 | NNNGCACAGCTTCAAGCCAG | NCA | 2 | 0.0 | Tier II |
54 | NC_000067.6 | 1 | 162128262 | + | Dnm3 | NNNGCACAGCTTCAATACAG | NGG | 2 | 0.0 | Tier II |
55 | NC_000073.6 | 7 | 89649674 | - | Me3 | NNNGCTCAGCTTCAATCCAG | NGG | 2 | 0.0 | Tier II |
56 | NC_000081.6 | 15 | 30681145 | + | Gm31841 | NNNGCACAGCTTCAAGCCAT | NGG | 1 | 0.7 | Tier III |
57 | NC_000071.6 | 5 | 28905870 | + | Gm35688 | NNNGCACAGCTTCAAGCTAG | NGG | 1 | 0.6429 | Tier III |
58 | NC_000067.6 | 1 | 165835541 | - | Gm32999 | NNNGCACAGGTTCAAACCAG | NGG | 2 | 0.3889 | Tier III |
59 | NC_000071.6 | 5 | 29271947 | - | Gm42244 | NNNGCACAGGTTCAAGCCAG | NGG | 1 | 0.3889 | Tier III |
60 | NC_000072.6 | 6 | 66258862 | + | Gm36408 | NNNTCCCAGCTTCAAGCCAG | NGG | 2 | 0.1653 | Tier III |
61 | NC_000070.6 | 4 | 107768061 | + | Gm12907 | NNNGAACAGCTTCAAGCCAG | NAG | 2 | 0.1481 | Tier III |
62 | NC_000082.6 | 16 | 31912448 | + | Gm34476 | NNNGCACAGGTTCAAGCCAG | NCG | 2 | 0.0417 | Tier III |
63 | NC_000075.6 | 9 | 115510884 | + | Gm39447 | NNNGCACAGCTTCAAGGCAT | NGG | 2 | 0.0412 | Tier III |
64 | NC_000073.6 | 7 | 63841291 | - | Gm39023 | NNNGCACAGATTCAAGCCAG | NTG | 2 | 0.0338 | Tier III |
65 | NC_000078.6 | 12 | 58644027 | + | Gm30687 | NNNGCACAGATTCAAGCCAG | NTG | 2 | 0.0338 | Tier III |
66 | NC_000073.6 | 7 | 117279389 | + | Gm39075 | NNNGCACACCTTCAAGGCAG | NGG | 2 | 0.0317 | Tier III |
67 | NC_000067.6 | 1 | 4587363 | - | Gm37323 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
68 | NC_000072.6 | 6 | 115509089 | + | Gm36301 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
69 | NC_000075.6 | 9 | 56314682 | + | Peak1os | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
70 | NC_000081.6 | 15 | 18195642 | + | 4921515E04Rik | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
71 | NC_000082.6 | 16 | 38837606 | - | Gm15802 | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
72 | NC_000083.6 | 17 | 66719346 | + | 1700016K05Rik | NNNGCACAGGTTCAAGCCAG | NTG | 2 | 0.0152 | Tier III |
73 | NC_000077.6 | 11 | 102900694 | + | Gm34560 | NNNGCTCAGCTTCAAGCCAG | NGT | 2 | 0.0115 | Tier III |
74 | NC_000077.6 | 11 | 102900694 | + | Gm39395 | NNNGCTCAGCTTCAAGCCAG | NGT | 2 | 0.0115 | Tier III |
75 | NC_000069.6 | 3 | 87489301 | + | Gm40085 | NNNGCACAGCTTCAAGACAG | NGT | 2 | 0.0075 | Tier III |
76 | NC_000073.6 | 7 | 138790792 | + | Gm30874 | NNNGCACAGCTTCAAGTCAG | NGT | 2 | 0.0075 | Tier III |
77 | NC_000080.6 | 14 | 25110629 | + | Gm32123 | NNNGCACAGCTTTAACCCAG | NGG | 2 | 0.0 | Tier III |