Construct: sgRNA BRDN0001147486

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGCACAGCTTCAAGCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPB8 (26353)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75642

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 119179632 + HSPB8 NNNGCACAGCTTCAAGCCAG NGG 0 1.0 Tier I
2 NC_000016.10 16 29697123 + QPRT NNNGGACAACTTCAAGCCAG NGG 2 0.3857 Tier I
3 NC_000019.10 19 2876115 + ZNF556 NNNGCTCAGCTTAAAGCCAG NGG 2 0.2747 Tier I
4 NC_000002.12 2 186722846 - FAM171B NNNGCACAACTCCAAGCCAG NGG 2 0.609 Tier II
5 NC_000006.12 6 107755520 - SCML4 NNNGCAAAGCTTCTAGCCAG NGG 2 0.4 Tier II
6 NC_000013.11 13 70016776 + KLHL1 NNNGCTCACCTTCAAGCCAG NGG 2 0.3846 Tier II
7 NC_000002.12 2 10830206 + PDIA6 NNNGCGCAGCTTCAAGACAG NGG 2 0.3333 Tier II
8 NC_000001.11 1 153643452 + CHTOP NNNGCACAGCTTCAAGTCAT NGG 2 0.3267 Tier II
9 NC_000012.12 12 76470712 + OSBPL8 NNNGCACAGCATCAAGCCAC NGG 2 0.3214 Tier II
10 NC_000016.10 16 28580499 - SGF29 NNNGGACAGCTGCAAGCCAG NGG 2 0.3 Tier II
11 NC_000015.10 15 29089627 - APBA2 NNNCCACAGCTTCAAGCCTG NGG 2 0.2851 Tier II
12 NC_000022.11 22 24370341 + SPECC1L NNNTCTCAGCTTCAAGCCAG NGG 2 0.2597 Tier II
13 NC_000023.11 X 72346447 + HDAC8 NNNGCACTGCTTCAAGCCTG NGG 2 0.2308 Tier II
14 NC_000015.10 15 40228850 - BUB1B-PAK6 NNNGCCCAGCTGCAAGCCAG NGG 2 0.2273 Tier II
15 NC_000002.12 2 230878355 - ITM2C NNNGCCCAGCTTCAAGACAG NGG 2 0.2121 Tier II
16 NC_000004.12 4 52903487 - SCFD2 NNNGCACAGCTTCAAGTCAC NGG 2 0.2 Tier II
17 NC_000008.11 8 139865050 + TRAPPC9 NNNGCACCGGTTCAAGCCAG NGG 2 0.1667 Tier II
18 NC_000023.11 X 152899532 - ZNF185 NNNGCACAGCATCCAGCCAG NGG 2 0.1607 Tier II
19 NC_000002.12 2 120276200 - RALB NNNGCACAGCTGCATGCCAG NGG 2 0.1 Tier II
20 NC_000002.12 2 127566090 + MYO7B NNNGCACAGCCTGAAGCCAG NGG 2 0.0909 Tier II
21 NC_000002.12 2 127659875 - LIMS2 NNNGCACAGCTGGAAGCCAG NGG 2 0.0682 Tier II
22 NC_000015.10 15 45659608 - SQOR NNNGCACAGCTACAAGCCAG NGA 2 0.0556 Tier II
23 NC_000009.12 9 130317212 - HMCN2 NNNTCACAGCTTGAAGCCAG NGG 2 0.0496 Tier II
24 NC_000002.12 2 21026214 + APOB NNNGCACAGCTTCAAGACAG NGA 2 0.0324 Tier II
25 NC_000020.11 20 38234921 - KIAA1755 NNNGCACAGCTTCAAGCCAC NGT 2 0.0069 Tier II
26 NC_000018.10 18 55358022 + TCF4 NNNGCACAGCTTCAAGCCAG NCT 2 0.0 Tier II
27 NC_000005.10 5 72808942 + TNPO1-DT NNNGCAAAGTTTCAAGCCAG NGG 2 0.7059 Tier III
28 NC_000013.11 13 30889197 - TEX26-AS1 NNNGCACAGCCTCAAGCCAA NGG 2 0.625 Tier III
29 NC_000005.10 5 152220079 + LINC01933 NNNGCACAGCTCCAGGCCAG NGG 2 0.6158 Tier III
30 NC_000023.11 X 39227110 + LOC105373175 NNNGGACAGCTCCAAGCCAG NGG 2 0.5684 Tier III
31 NC_000002.12 2 119744399 + LOC101927764 NNNGAAGAGCTTCAAGCCAG NGG 2 0.2689 Tier III
32 NC_000022.11 22 24370341 + SPECC1L-ADORA2A NNNTCTCAGCTTCAAGCCAG NGG 2 0.2597 Tier III
33 NC_000010.11 10 65779455 + LOC105378339 NNNCCCCAGCTTCAAGCCAG NGG 2 0.2406 Tier III
34 NC_000015.10 15 40228850 - LOC107984763 NNNGCCCAGCTGCAAGCCAG NGG 2 0.2273 Tier III
35 NC_000008.11 8 139865050 + LOC107986981 NNNGCACCGGTTCAAGCCAG NGG 2 0.1667 Tier III
36 NC_000002.12 2 127566090 + LOC105373609 NNNGCACAGCCTGAAGCCAG NGG 2 0.0909 Tier III
37 NC_000006.12 6 14992878 + LOC105374945 NNNGGACAGCTTGAAGCCAG NGG 2 0.0818 Tier III
38 NC_000019.10 19 16038560 - LINC00905 NNNGCACAGCTGGAAGCCAG NGG 2 0.0682 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 88889802 - Mcm2 NNNGGACAGCTGCAAGCCAG NGG 2 0.3 Tier I
2 NC_000071.6 5 116422161 - Hspb8 NNNGCACAGCTTCAAGCCGG NAG 2 0.0972 Tier I
3 NC_000069.6 3 153706749 - St6galnac3 NNNACACAGATTCAAGCCAG NGG 2 0.78 Tier II
4 NC_000081.6 15 30681145 + Ctnnd2 NNNGCACAGCTTCAAGCCAT NGG 1 0.7 Tier II
5 NC_000073.6 7 118889207 - Iqck NNNACAAAGCTTCAAGCCAG NGG 2 0.675 Tier II
6 NC_000068.7 2 68971223 - Cers6 NNNGGAAAGCTTCAAGCCAG NGG 2 0.45 Tier II
7 NC_000078.6 12 89362632 - Nrxn3 NNNGCATAGCTTCAAGCAAG NGG 2 0.4375 Tier II
8 NC_000067.6 1 165835541 - Cd247 NNNGCACAGGTTCAAACCAG NGG 2 0.3889 Tier II
9 NC_000071.6 5 29271947 - Lmbr1 NNNGCACAGGTTCAAGCCAG NGG 1 0.3889 Tier II
10 NC_000069.6 3 90502030 - Chtop NNNGCACAGCTTCAAGTCAT NGG 2 0.3267 Tier II
11 NC_000086.7 X 20144562 - Slc9a7 NNNGTACAGCTTCAAGTCAG NGG 2 0.297 Tier II
12 NC_000068.7 2 109105998 - Mettl15 NNNGCTCAGGTTCAAGCCAG NGG 2 0.2778 Tier II
13 NC_000082.6 16 40845030 + Lsamp NNNGCACAAGTTCAAGCCAG NGG 2 0.25 Tier II
14 NC_000067.6 1 127339210 - Mgat5 NNNGCAGAGCTGCAAGCCAG NGG 2 0.2353 Tier II
15 NC_000069.6 3 76540231 - Fstl5 NNNGCACAGGTTCAAGCCAG NAG 2 0.1008 Tier II
16 NC_000072.6 6 13655959 - Bmt2 NNNGCACAGGTTCAAGCCAG NAG 2 0.1008 Tier II
17 NC_000081.6 15 5113536 + Card6 NNNGCACAGGTTCAAGCCAG NAG 2 0.1008 Tier II
18 NC_000084.6 18 62558993 - Spink10 NNNGCACAGGTTCAAGCCAG NAG 2 0.1008 Tier II
19 NC_000067.6 1 135265118 - Rnpep NNNGCACAGCTTCATGTCAG NGG 2 0.0933 Tier II
20 NC_000080.6 14 99197665 - Pibf1 NNNGCACAGGTTCCAGCCAG NGG 2 0.0833 Tier II
21 NC_000080.6 14 64300231 - Msra NNNGCACAGCTTGAAGCCAC NGG 2 0.0584 Tier II
22 NC_000081.6 15 21496231 - Cdh12 NNNGCACAGCTTTAAGCCAG NGA 2 0.0486 Tier II
23 NC_000072.6 6 86953968 - Aak1 NNNGCACAGGTTCAAGCCAG NCG 2 0.0417 Tier II
24 NC_000074.6 8 86650982 - Lonp2 NNNGCACAGGTTCAAGCCAG NCG 2 0.0417 Tier II
25 NC_000083.6 17 68329968 - L3mbtl4 NNNGCACAGGTTCAAGCCAG NCG 2 0.0417 Tier II
26 NC_000074.6 8 65796245 - Marchf1 NNNGCACAGATTCAAGCCAG NTG 2 0.0338 Tier II
27 NC_000068.7 2 68438156 - Stk39 NNNGCAGAGCTTCAAGCCAG NGA 2 0.0327 Tier II
28 NC_000080.6 14 10059548 - Fhit NNNGCTCAGCTTCAAGCCAG NTG 2 0.0278 Tier II
29 NC_000083.6 17 75505902 - Rasgrp3 NNNGCACAGGTTCAAGGCAG NGG 2 0.0229 Tier II
30 NC_000085.6 19 52983813 + Xpnpep1 NNNGCACTGCTTCAAGCCAG NTG 2 0.0167 Tier II
31 NC_000079.6 13 106931140 + Ipo11 NNNGCACAGCTTCAAGCCAG NGT 1 0.0161 Tier II
32 NC_000067.6 1 53325341 + Osgepl1 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
33 NC_000067.6 1 187106580 + Spata17 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
34 NC_000070.6 4 98618646 - Patj NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
35 NC_000072.6 6 13656636 - Bmt2 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
36 NC_000072.6 6 28270051 - Zfp800 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
37 NC_000075.6 9 56314682 + Peak1 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
38 NC_000075.6 9 67881133 - Vps13c NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
39 NC_000078.6 12 83870233 + Numb NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
40 NC_000081.6 15 53819550 + Samd12 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
41 NC_000081.6 15 94825660 - Tmem117 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
42 NC_000082.6 16 31928452 - Pigz NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
43 NC_000083.6 17 66719346 + Ptprm NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
44 NC_000086.7 X 36944710 + Septin6 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier II
45 NC_000075.6 9 15304648 + 4931406C07Rik NNNGCACAGCTTCCAGCCAG NTG 2 0.0083 Tier II
46 NC_000069.6 3 87489301 + Fcrl5 NNNGCACAGCTTCAAGACAG NGT 2 0.0075 Tier II
47 NC_000080.6 14 75088242 - Lrrc63 NNNGCACAGGTTCAAGCCAG NGT 2 0.0063 Tier II
48 NC_000085.6 19 5230228 - Pacs1 NNNGCACAGGTTCAAGCCAG NGT 2 0.0063 Tier II
49 NC_000067.6 1 51064800 + Tmeff2 NNNTCACAGCTTCAAGCCAG NGT 2 0.0059 Tier II
50 NC_000079.6 13 15485706 + Gli3 NNNGCACAGCTACAACCCAG NGG 2 0.0 Tier II
51 NC_000072.6 6 48836405 + Tmem176b NNNGCACAGCTCCAACCCAG NGG 2 0.0 Tier II
52 NC_000068.7 2 49720142 - Kif5c NNNGCACAGCTTCAACCCAG NGA 2 0.0 Tier II
53 NC_000077.6 11 5626517 + Ankrd36 NNNGCACAGCTTCAAGCCAG NCA 2 0.0 Tier II
54 NC_000067.6 1 162128262 + Dnm3 NNNGCACAGCTTCAATACAG NGG 2 0.0 Tier II
55 NC_000073.6 7 89649674 - Me3 NNNGCTCAGCTTCAATCCAG NGG 2 0.0 Tier II
56 NC_000081.6 15 30681145 + Gm31841 NNNGCACAGCTTCAAGCCAT NGG 1 0.7 Tier III
57 NC_000071.6 5 28905870 + Gm35688 NNNGCACAGCTTCAAGCTAG NGG 1 0.6429 Tier III
58 NC_000067.6 1 165835541 - Gm32999 NNNGCACAGGTTCAAACCAG NGG 2 0.3889 Tier III
59 NC_000071.6 5 29271947 - Gm42244 NNNGCACAGGTTCAAGCCAG NGG 1 0.3889 Tier III
60 NC_000072.6 6 66258862 + Gm36408 NNNTCCCAGCTTCAAGCCAG NGG 2 0.1653 Tier III
61 NC_000070.6 4 107768061 + Gm12907 NNNGAACAGCTTCAAGCCAG NAG 2 0.1481 Tier III
62 NC_000082.6 16 31912448 + Gm34476 NNNGCACAGGTTCAAGCCAG NCG 2 0.0417 Tier III
63 NC_000075.6 9 115510884 + Gm39447 NNNGCACAGCTTCAAGGCAT NGG 2 0.0412 Tier III
64 NC_000073.6 7 63841291 - Gm39023 NNNGCACAGATTCAAGCCAG NTG 2 0.0338 Tier III
65 NC_000078.6 12 58644027 + Gm30687 NNNGCACAGATTCAAGCCAG NTG 2 0.0338 Tier III
66 NC_000073.6 7 117279389 + Gm39075 NNNGCACACCTTCAAGGCAG NGG 2 0.0317 Tier III
67 NC_000067.6 1 4587363 - Gm37323 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
68 NC_000072.6 6 115509089 + Gm36301 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
69 NC_000075.6 9 56314682 + Peak1os NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
70 NC_000081.6 15 18195642 + 4921515E04Rik NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
71 NC_000082.6 16 38837606 - Gm15802 NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
72 NC_000083.6 17 66719346 + 1700016K05Rik NNNGCACAGGTTCAAGCCAG NTG 2 0.0152 Tier III
73 NC_000077.6 11 102900694 + Gm34560 NNNGCTCAGCTTCAAGCCAG NGT 2 0.0115 Tier III
74 NC_000077.6 11 102900694 + Gm39395 NNNGCTCAGCTTCAAGCCAG NGT 2 0.0115 Tier III
75 NC_000069.6 3 87489301 + Gm40085 NNNGCACAGCTTCAAGACAG NGT 2 0.0075 Tier III
76 NC_000073.6 7 138790792 + Gm30874 NNNGCACAGCTTCAAGTCAG NGT 2 0.0075 Tier III
77 NC_000080.6 14 25110629 + Gm32123 NNNGCACAGCTTTAACCCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)