Construct: sgRNA BRDN0001147487
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTACAGAGCTCTCAGCATGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- DCLK1 (9201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000013.11 | 13 | 35839166 | - | DCLK1 | NNNCAGAGCTCTCAGCATGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 223110667 | - | TLR5 | NNNCAGTGCTCTCATCATGG | NGG | 2 | 0.0625 | Tier I |
3 | NC_000002.12 | 2 | 169827509 | + | UBR3 | NNNCTGAGCTCTCATCATGG | NGG | 2 | 0.0519 | Tier I |
4 | NC_000016.10 | 16 | 12962029 | + | SHISA9 | NNNCAGAGCTCCCAGCATGG | NGG | 1 | 0.9474 | Tier II |
5 | NC_000012.12 | 12 | 109491824 | - | UBE3B | NNNCAGAGCTCTCAGCATGA | NGG | 1 | 0.9375 | Tier II |
6 | NC_000009.12 | 9 | 36265357 | - | GNE | NNNCAGAGCTCCCAACATGG | NGG | 2 | 0.8916 | Tier II |
7 | NC_000007.14 | 7 | 56090524 | - | PHKG1 | NNNCAGAGCACCCAGCATGG | NGG | 2 | 0.812 | Tier II |
8 | NC_000017.11 | 17 | 82033188 | + | RAC3 | NNNCACAGCTCTCAGAATGG | NGG | 2 | 0.6818 | Tier II |
9 | NC_000015.10 | 15 | 41836080 | + | JMJD7-PLA2G4B | NNNCAGGGCTCTCAGCATGA | NGG | 2 | 0.6618 | Tier II |
10 | NC_000015.10 | 15 | 41836080 | + | JMJD7 | NNNCAGGGCTCTCAGCATGA | NGG | 2 | 0.6618 | Tier II |
11 | NC_000011.10 | 11 | 62639767 | + | GANAB | NNNCAGAGCAATCAGCATGG | NGG | 2 | 0.6429 | Tier II |
12 | NC_000013.11 | 13 | 46369819 | + | RUBCNL | NNNCACAGCTCTCAGCATGA | NGG | 2 | 0.6392 | Tier II |
13 | NC_000002.12 | 2 | 239112764 | - | HDAC4 | NNNCAGAGCTCCCAGCAAGG | NGG | 2 | 0.6316 | Tier II |
14 | NC_000012.12 | 12 | 117763462 | - | KSR2 | NNNTAGAGCTCTTAGCATGG | NGG | 2 | 0.56 | Tier II |
15 | NC_000001.11 | 1 | 209585272 | - | CAMK1G | NNNCAGATTTCTCAGCATGG | NGG | 2 | 0.5469 | Tier II |
16 | NC_000017.11 | 17 | 30228938 | + | SLC6A4 | NNNTACAGCTCTCAGCATGG | NGG | 2 | 0.5455 | Tier II |
17 | NC_000008.11 | 8 | 132302822 | + | KCNQ3 | NNNAAGACCTCTCAGCATGG | NGG | 2 | 0.5182 | Tier II |
18 | NC_000001.11 | 1 | 17586901 | - | ARHGEF10L | NNNCCAAGCTCTCAGCATGG | NGG | 2 | 0.5 | Tier II |
19 | NC_000001.11 | 1 | 41482883 | + | EDN2 | NNNGAGAGCTCCCAGCATGG | NGG | 2 | 0.4737 | Tier II |
20 | NC_000007.14 | 7 | 39264100 | + | POU6F2 | NNNCAGGCCTCTCAGCATGG | NGG | 2 | 0.4344 | Tier II |
21 | NC_000015.10 | 15 | 29291598 | + | ENTREP2 | NNNCCCAGCTCTCAGCATGG | NGG | 2 | 0.3409 | Tier II |
22 | NC_000018.10 | 18 | 46547597 | + | LOXHD1 | NNNCAGACCCCTCAGCATGG | NGG | 2 | 0.3282 | Tier II |
23 | NC_000011.10 | 11 | 10605662 | - | IRAG1 | NNNCACAGCTCTCGGCATGG | NGG | 2 | 0.3182 | Tier II |
24 | NC_000020.11 | 20 | 47521147 | - | NCOA3 | NNNCTGAGCTCTCAGCAAGG | NGG | 2 | 0.2424 | Tier II |
25 | NC_000008.11 | 8 | 3259373 | + | CSMD1 | NNNCTTAGCTCTCAGCATGG | NGG | 2 | 0.2424 | Tier II |
26 | NC_000007.14 | 7 | 47277426 | + | TNS3 | NNNCAGAGCACTCACCATGG | NGG | 2 | 0.2338 | Tier II |
27 | NC_000011.10 | 11 | 72649210 | - | PDE2A | NNNCAGAGCTGACAGCATGG | NGG | 2 | 0.2 | Tier II |
28 | NC_000002.12 | 2 | 196822309 | - | C2orf66 | NNNCAGTGCTCTCAGCATGC | NGG | 2 | 0.1875 | Tier II |
29 | NC_000007.14 | 7 | 103562756 | + | RELN | NNNCACAGCTGTCAGCATGG | NGG | 2 | 0.1705 | Tier II |
30 | NC_000010.11 | 10 | 131178138 | - | TCERG1L | NNNCAGAGCGCTCAGCAGGG | NGG | 2 | 0.1667 | Tier II |
31 | NC_000015.10 | 15 | 76148985 | - | TMEM266 | NNNCAGAACTCTCAGGATGG | NGG | 2 | 0.1538 | Tier II |
32 | NC_000018.10 | 18 | 63709217 | - | SERPINB11 | NNNCAGAGATCTCAGCCTGG | NGG | 2 | 0.1513 | Tier II |
33 | NC_000005.10 | 5 | 149378492 | + | IL17B | NNNCAGAGCTCCCAGGATGG | NGG | 2 | 0.1457 | Tier II |
34 | NC_000005.10 | 5 | 139587014 | + | UBE2D2 | NNNCAGAGCTCTCAAGATGG | NGG | 2 | 0.1448 | Tier II |
35 | NC_000006.12 | 6 | 29558953 | - | UBD | NNNCAGAGCTCTCAAGATGG | NGG | 2 | 0.1448 | Tier II |
36 | NC_000017.11 | 17 | 38504465 | - | ARHGAP23 | NNNCAGAGCTCACAGCCTGG | NGG | 2 | 0.1412 | Tier II |
37 | NC_000011.10 | 11 | 48074882 | + | PTPRJ | NNNCAGTGCTCTCAGCATGG | NAG | 2 | 0.1134 | Tier II |
38 | NC_000002.12 | 2 | 26568047 | - | CIMIP2C | NNNCAGACCTCTCAGCCTGG | NGG | 2 | 0.1086 | Tier II |
39 | NC_000007.14 | 7 | 73557077 | + | BCL7B | NNNCAGAGCGCTCAGCCTGG | NGG | 2 | 0.0882 | Tier II |
40 | NC_000014.9 | 14 | 23550926 | - | THTPA | NNNCAGAGCTGTCAGCCTGG | NGG | 2 | 0.0441 | Tier II |
41 | NC_000014.9 | 14 | 23550926 | - | ZFHX2 | NNNCAGAGCTGTCAGCCTGG | NGG | 2 | 0.0441 | Tier II |
42 | NC_000003.12 | 3 | 133881188 | - | RAB6B | NNNCAGAGCTCGCAGCATGG | NGA | 2 | 0.0347 | Tier II |
43 | NC_000004.12 | 4 | 158162314 | - | GASK1B | NNNCAGAGCTCTCAGCTTGG | NAG | 2 | 0.0346 | Tier II |
44 | NC_000020.11 | 20 | 59309282 | - | EDN3 | NNNCAGAGCTCTCAGCATAG | NTG | 2 | 0.0278 | Tier II |
45 | NC_000002.12 | 2 | 236130945 | + | AGAP1 | NNNCAGGGCTCTCAGCATGG | NTG | 2 | 0.0275 | Tier II |
46 | NC_000003.12 | 3 | 61113532 | - | FHIT | NNNCAAAGCTCTCAGCATGG | NGC | 2 | 0.0222 | Tier II |
47 | NC_000007.14 | 7 | 32417422 | - | PDE1C | NNNCAAAGCTCTCAGCATGG | NGC | 2 | 0.0222 | Tier II |
48 | NC_000011.10 | 11 | 67583896 | + | GSTP1 | NNNCAGAGCTCCCAGCATGG | NGC | 2 | 0.0211 | Tier II |
49 | NC_000002.12 | 2 | 3613422 | + | COLEC11 | NNNCAGAGCTCTGAGGATGG | NGG | 2 | 0.021 | Tier II |
50 | NC_000022.11 | 22 | 17855014 | - | MICAL3 | NNNCAGAGCTCTCAGGTTGG | NGG | 2 | 0.0205 | Tier II |
51 | NC_000002.12 | 2 | 9314526 | - | ASAP2 | NNNCAGAGCTCTTAGCATGG | NGC | 2 | 0.0156 | Tier II |
52 | NC_000002.12 | 2 | 109180906 | - | RANBP2 | NNNCAGAGCTCTCTGCATGG | NGT | 2 | 0.0086 | Tier II |
53 | NC_000002.12 | 2 | 109180906 | - | SH3RF3 | NNNCAGAGCTCTCTGCATGG | NGT | 2 | 0.0086 | Tier II |
54 | NC_000004.12 | 4 | 114967475 | + | NDST4 | NNNCAGAGCTCTCAGCATGG | NTC | 2 | 0.0 | Tier II |
55 | NC_000009.12 | 9 | 133222172 | + | OBP2B | NNNCAGAGCTCTCAGCATGG | NTC | 2 | 0.0 | Tier II |
56 | NC_000015.10 | 15 | 22883041 | + | CYFIP1 | NNNCAGAGCTCTCAGCATGG | NCC | 2 | 0.0 | Tier II |
57 | NC_000001.11 | 1 | 228942860 | - | LOC124904539 | NNNCAGAGCTCCCAACATGG | NGG | 2 | 0.8916 | Tier III |
58 | NC_000001.11 | 1 | 228954261 | - | LOC105373143 | NNNCAGAGCTCCCAACATGG | NGG | 2 | 0.8916 | Tier III |
59 | NC_000009.12 | 9 | 36265357 | - | LOC124902150 | NNNCAGAGCTCCCAACATGG | NGG | 2 | 0.8916 | Tier III |
60 | NC_000001.11 | 1 | 209585272 | - | LOC107985256 | NNNCAGATTTCTCAGCATGG | NGG | 2 | 0.5469 | Tier III |
61 | NC_000010.11 | 10 | 121076338 | + | LOC105378521 | NNNCAGGGCTCTCAGCATTG | NGG | 2 | 0.4706 | Tier III |
62 | NC_000009.12 | 9 | 93808515 | + | LOC101928014 | NNNCAGAGCTCTCAGCATCA | NGG | 2 | 0.4203 | Tier III |
63 | NC_000017.11 | 17 | 83207425 | + | LOC101929650 | NNNGGGAGCTCTCAGCATGG | NGG | 2 | 0.36 | Tier III |
64 | NC_000005.10 | 5 | 173707749 | + | LINC01484 | NNNCAGAGCTCTCAGCATGG | NAG | 1 | 0.2593 | Tier III |
65 | NC_000001.11 | 1 | 207804971 | - | MIR29B2CHG | NNNCAGAGCTCTCAGCAGGC | NGG | 2 | 0.1429 | Tier III |
66 | NC_000002.12 | 2 | 164848335 | - | LOC101929633 | NNNCAGAGCTCTCAGCTTGG | NGG | 1 | 0.1333 | Tier III |
67 | NC_000023.11 | X | 132984747 | - | LOC124905219 | NNNCAGAGCTCACAGGATGG | NGG | 2 | 0.1231 | Tier III |
68 | NC_000008.11 | 8 | 126867220 | + | LOC105375751 | NNNCACAGCTCTCAGCATGG | NCG | 2 | 0.0731 | Tier III |
69 | NC_000022.11 | 22 | 49047134 | + | NHIP | NNNCTGAGCTCTCAGCTTGG | NGG | 2 | 0.0485 | Tier III |
70 | NC_000001.11 | 1 | 29503849 | + | LOC107984933 | NNNCAGAGCTCTCAGCCTGG | NGA | 2 | 0.0123 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000073.6 | 7 | 5488267 | + | Vmn2r28 | NNNCAGAAATCTCAGCATGG | NGG | 2 | 0.8571 | Tier I |
2 | NC_000069.6 | 3 | 55477816 | + | Dclk1 | NNNCAGATCTCTCAGCATGG | NGG | 1 | 0.625 | Tier I |
3 | NC_000081.6 | 15 | 78483952 | - | Il2rb | NNNCTGAGCTCCCAGCATGG | NGG | 2 | 0.3445 | Tier I |
4 | NC_000075.6 | 9 | 30921688 | + | Adamts15 | NNNCAGAGCCCTCAGGATGG | NGG | 2 | 0.0821 | Tier I |
5 | NC_000071.6 | 5 | 124741940 | - | Dnah10 | NNNCAGAGCGCTCATCATGG | NGG | 2 | 0.0714 | Tier I |
6 | NC_000068.7 | 2 | 69897296 | + | Ubr3 | NNNCTGAGCTCTCATCATGG | NGG | 2 | 0.0519 | Tier I |
7 | NC_000078.6 | 12 | 108675560 | - | Evl | NNNCAGAGCTCTCATCATGG | NTG | 2 | 0.0056 | Tier I |
8 | NC_000078.6 | 12 | 74025561 | - | Syt16 | NNNCAGAGCTCTCAACATGG | NGG | 1 | 0.9412 | Tier II |
9 | NC_000083.6 | 17 | 49871510 | + | Kif6 | NNNCAGAGCTCTCAGAATGA | NGG | 2 | 0.9375 | Tier II |
10 | NC_000074.6 | 8 | 120440152 | + | Gse1 | NNNCAGAGTTCCCAGCATGG | NGG | 2 | 0.8289 | Tier II |
11 | NC_000070.6 | 4 | 156185780 | - | Agrn | NNNCAGAGCACCCAGCATGG | NGG | 2 | 0.812 | Tier II |
12 | NC_000078.6 | 12 | 4951620 | - | Atad2b | NNNTAGAACTCTCAGCATGG | NGG | 2 | 0.8 | Tier II |
13 | NC_000077.6 | 11 | 33244099 | + | Ranbp17 | NNNAAGAGCTATCAGCATGG | NGG | 2 | 0.6316 | Tier II |
14 | NC_000085.6 | 19 | 40723877 | + | Entpd1 | NNNCAGAGCTCCCAGCATTG | NGG | 2 | 0.6316 | Tier II |
15 | NC_000078.6 | 12 | 80579049 | + | Galnt16 | NNNCAGAGGTCTCAGCATGG | NGG | 1 | 0.619 | Tier II |
16 | NC_000071.6 | 5 | 101872362 | + | Wdfy3 | NNNCAGAGCTAACAGCATGG | NGG | 2 | 0.6 | Tier II |
17 | NC_000067.6 | 1 | 56855407 | + | Satb2 | NNNCAGAGCTCTCTGCATGG | NGG | 1 | 0.5333 | Tier II |
18 | NC_000072.6 | 6 | 108066673 | - | Setmar | NNNAAGATCTCTCAGCATGG | NGG | 2 | 0.5263 | Tier II |
19 | NC_000078.6 | 12 | 83322003 | + | Dpf3 | NNNCATGGCTCTCAGCATGG | NGG | 2 | 0.4706 | Tier II |
20 | NC_000073.6 | 7 | 136933963 | - | Mgmt | NNNCAGAGACCTCAGCATGG | NGG | 2 | 0.4571 | Tier II |
21 | NC_000076.6 | 10 | 59766667 | - | Micu1 | NNNCAGTGCTCTCAGCATGG | NGG | 1 | 0.4375 | Tier II |
22 | NC_000079.6 | 13 | 19117785 | + | Amph | NNNCAGAGCACGCAGCATGG | NGG | 2 | 0.4286 | Tier II |
23 | NC_000077.6 | 11 | 118908628 | - | Rbfox3 | NNNCAGAGCCCTCAGCATAG | NGG | 2 | 0.381 | Tier II |
24 | NC_000083.6 | 17 | 44516687 | - | Runx2 | NNNCAGAGATCTCAGCATGC | NGG | 2 | 0.3673 | Tier II |
25 | NC_000083.6 | 17 | 23838192 | + | Prss41 | NNNCTGAACTCTCAGCATGG | NGG | 2 | 0.3636 | Tier II |
26 | NC_000069.6 | 3 | 56004029 | - | Nbea | NNNCCGAGCTCTCAGCATTG | NGG | 2 | 0.3333 | Tier II |
27 | NC_000067.6 | 1 | 120181504 | - | 3110009E18Rik | NNNCAGAGGTCTCTGCATGG | NGG | 2 | 0.3302 | Tier II |
28 | NC_000078.6 | 12 | 36535191 | + | Crppa | NNNCTGAGTTCTCAGCATGG | NGG | 2 | 0.3182 | Tier II |
29 | NC_000070.6 | 4 | 115835525 | - | Mob3c | NNNCAGAGGGCTCAGCATGG | NGG | 2 | 0.3095 | Tier II |
30 | NC_000075.6 | 9 | 32466861 | + | Fli1 | NNNCAGAGCTCTCAGCAAGC | NGG | 2 | 0.2857 | Tier II |
31 | NC_000071.6 | 5 | 125119741 | - | Ncor2 | NNNCAGGGCTCTAAGCATGG | NGG | 2 | 0.2715 | Tier II |
32 | NC_000073.6 | 7 | 49927461 | - | Slc6a5 | NNNCAGAGCTCTAAGCATTG | NGG | 2 | 0.2564 | Tier II |
33 | NC_000086.7 | X | 74276779 | + | Dnase1l1 | NNNCAGAGCTGTCAACATGG | NGG | 2 | 0.2353 | Tier II |
34 | NC_000071.6 | 5 | 125432010 | - | Dhx37 | NNNCAGGGCTCTCAGCATGG | NAG | 2 | 0.183 | Tier II |
35 | NC_000073.6 | 7 | 91092175 | + | Dlg2 | NNNCAGACCTCTCAGCATGG | NAG | 2 | 0.1595 | Tier II |
36 | NC_000069.6 | 3 | 13975722 | + | Ralyl | NNNCAGAGCTCCCAGCTTGG | NGG | 2 | 0.1263 | Tier II |
37 | NC_000077.6 | 11 | 24077408 | + | Bcl11a | NNNCAGAGCTCTCAACTTGG | NGG | 2 | 0.1255 | Tier II |
38 | NC_000086.7 | X | 162103925 | - | Nhs | NNNCAGAGCTCTTAGCCTGG | NGG | 2 | 0.1235 | Tier II |
39 | NC_000067.6 | 1 | 124557780 | - | Dpp10 | NNNCAGAGATCTGAGCATGG | NGG | 2 | 0.1169 | Tier II |
40 | NC_000081.6 | 15 | 28298017 | - | Dnah5 | NNNCAGAGCTCTCAGGAAGG | NGG | 2 | 0.1026 | Tier II |
41 | NC_000074.6 | 8 | 83237288 | - | Tbc1d9 | NNNCAGGGCTCTGAGCATGG | NGG | 2 | 0.0963 | Tier II |
42 | NC_000079.6 | 13 | 38405463 | + | Bmp6 | NNNCAGAGCTTTCAGTATGG | NGG | 2 | 0.0947 | Tier II |
43 | NC_000078.6 | 12 | 102265851 | + | Slc24a4 | NNNCAGAGCTCTCTGCTTGG | NGG | 2 | 0.0711 | Tier II |
44 | NC_000081.6 | 15 | 64884960 | - | Adcy8 | NNNCTGAGCTCTCAGCCTGG | NGG | 2 | 0.0642 | Tier II |
45 | NC_000081.6 | 15 | 21301422 | + | Cdh12 | NNNCAGAGCTCTGGGCATGG | NGG | 2 | 0.0636 | Tier II |
46 | NC_000073.6 | 7 | 82758616 | + | Efl1 | NNNCAGAGTTCTCAGCATGG | NGA | 2 | 0.0608 | Tier II |
47 | NC_000070.6 | 4 | 137729031 | + | Rap1gap | NNNCAGAGCTCTCAGAATGG | NTG | 2 | 0.039 | Tier II |
48 | NC_000069.6 | 3 | 125576026 | - | Ndst4 | NNNCAGAGCTCTCAGCATGG | NTG | 1 | 0.039 | Tier II |
49 | NC_000078.6 | 12 | 116535645 | - | Ptprn2 | NNNCAGAGCACTCAGCATGG | NTG | 2 | 0.0334 | Tier II |
50 | NC_000078.6 | 12 | 41048895 | + | Immp2l | NNNCAGAGCTCTCTGCATGG | NTG | 2 | 0.0208 | Tier II |
51 | NC_000067.6 | 1 | 39440454 | + | Tbc1d8 | NNNCAGAGCTCTCAGCATGC | NGC | 2 | 0.0095 | Tier II |
52 | NC_000084.6 | 18 | 69602536 | + | Tcf4 | NNNCAGAGCTCTCAGCATGC | NGC | 2 | 0.0095 | Tier II |
53 | NC_000084.6 | 18 | 15092560 | - | Kctd1 | NNNCTGAGCTCTCAGCATGG | NGC | 2 | 0.0081 | Tier II |
54 | NC_000077.6 | 11 | 118514629 | + | Rbfox3 | NNNCAGAGCTCTCAGCCTGG | NTG | 2 | 0.0069 | Tier II |
55 | NC_000084.6 | 18 | 13811626 | - | Zfp521 | NNNCAGAGCTCTCAGGATGG | NGT | 2 | 0.0025 | Tier II |
56 | NC_000073.6 | 7 | 7714589 | + | Vmn2r-ps38 | NNNCAGAAATCTCAGCATGG | NGG | 2 | 0.8571 | Tier III |
57 | NC_000073.6 | 7 | 8514207 | + | Vmn2r-ps47 | NNNCAGAAATCTCAGCATGG | NGG | 2 | 0.8571 | Tier III |
58 | NC_000073.6 | 7 | 9248807 | + | Gm4011 | NNNCAGAAATCTCAGCATGG | NGG | 2 | 0.8571 | Tier III |
59 | NC_000073.6 | 7 | 19752607 | - | Gm34744 | NNNCAAGGCTCTCAGCATGG | NGG | 2 | 0.7059 | Tier III |
60 | NC_000081.6 | 15 | 74302329 | + | Gm19859 | NNNCAGGGCTCCCAGCATGG | NGG | 2 | 0.6687 | Tier III |
61 | NC_000069.6 | 3 | 55477816 | + | Gm31443 | NNNCAGATCTCTCAGCATGG | NGG | 1 | 0.625 | Tier III |
62 | NC_000069.6 | 3 | 56004029 | - | Gm31914 | NNNCCGAGCTCTCAGCATTG | NGG | 2 | 0.3333 | Tier III |
63 | NC_000067.6 | 1 | 102264758 | + | Gm3116 | NNNCAGAGCTCCCAGTATGG | NGG | 2 | 0.2915 | Tier III |
64 | NC_000085.6 | 19 | 43642040 | + | Gm34922 | NNNCAGAGCTCTCAGGAAGG | NGG | 2 | 0.1026 | Tier III |
65 | NC_000069.6 | 3 | 38681251 | - | Gm29879 | NNNCAGAGCTCTGAGCAAGG | NGG | 2 | 0.0909 | Tier III |
66 | NC_000082.6 | 16 | 49767711 | + | Gm15518 | NNNCAGAGCTCCCAGCATGG | NTG | 2 | 0.0369 | Tier III |
67 | NC_000076.6 | 10 | 61829682 | + | Gm51765 | NNNCAGAGCTCTCTGCATGG | NTG | 2 | 0.0208 | Tier III |