Construct: sgRNA BRDN0001147487

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTACAGAGCTCTCAGCATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCLK1 (9201)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76747

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 35839166 - DCLK1 NNNCAGAGCTCTCAGCATGG NGG 0 1.0 Tier I
2 NC_000001.11 1 223110667 - TLR5 NNNCAGTGCTCTCATCATGG NGG 2 0.0625 Tier I
3 NC_000002.12 2 169827509 + UBR3 NNNCTGAGCTCTCATCATGG NGG 2 0.0519 Tier I
4 NC_000016.10 16 12962029 + SHISA9 NNNCAGAGCTCCCAGCATGG NGG 1 0.9474 Tier II
5 NC_000012.12 12 109491824 - UBE3B NNNCAGAGCTCTCAGCATGA NGG 1 0.9375 Tier II
6 NC_000009.12 9 36265357 - GNE NNNCAGAGCTCCCAACATGG NGG 2 0.8916 Tier II
7 NC_000007.14 7 56090524 - PHKG1 NNNCAGAGCACCCAGCATGG NGG 2 0.812 Tier II
8 NC_000017.11 17 82033188 + RAC3 NNNCACAGCTCTCAGAATGG NGG 2 0.6818 Tier II
9 NC_000015.10 15 41836080 + JMJD7-PLA2G4B NNNCAGGGCTCTCAGCATGA NGG 2 0.6618 Tier II
10 NC_000015.10 15 41836080 + JMJD7 NNNCAGGGCTCTCAGCATGA NGG 2 0.6618 Tier II
11 NC_000011.10 11 62639767 + GANAB NNNCAGAGCAATCAGCATGG NGG 2 0.6429 Tier II
12 NC_000013.11 13 46369819 + RUBCNL NNNCACAGCTCTCAGCATGA NGG 2 0.6392 Tier II
13 NC_000002.12 2 239112764 - HDAC4 NNNCAGAGCTCCCAGCAAGG NGG 2 0.6316 Tier II
14 NC_000012.12 12 117763462 - KSR2 NNNTAGAGCTCTTAGCATGG NGG 2 0.56 Tier II
15 NC_000001.11 1 209585272 - CAMK1G NNNCAGATTTCTCAGCATGG NGG 2 0.5469 Tier II
16 NC_000017.11 17 30228938 + SLC6A4 NNNTACAGCTCTCAGCATGG NGG 2 0.5455 Tier II
17 NC_000008.11 8 132302822 + KCNQ3 NNNAAGACCTCTCAGCATGG NGG 2 0.5182 Tier II
18 NC_000001.11 1 17586901 - ARHGEF10L NNNCCAAGCTCTCAGCATGG NGG 2 0.5 Tier II
19 NC_000001.11 1 41482883 + EDN2 NNNGAGAGCTCCCAGCATGG NGG 2 0.4737 Tier II
20 NC_000007.14 7 39264100 + POU6F2 NNNCAGGCCTCTCAGCATGG NGG 2 0.4344 Tier II
21 NC_000015.10 15 29291598 + ENTREP2 NNNCCCAGCTCTCAGCATGG NGG 2 0.3409 Tier II
22 NC_000018.10 18 46547597 + LOXHD1 NNNCAGACCCCTCAGCATGG NGG 2 0.3282 Tier II
23 NC_000011.10 11 10605662 - IRAG1 NNNCACAGCTCTCGGCATGG NGG 2 0.3182 Tier II
24 NC_000020.11 20 47521147 - NCOA3 NNNCTGAGCTCTCAGCAAGG NGG 2 0.2424 Tier II
25 NC_000008.11 8 3259373 + CSMD1 NNNCTTAGCTCTCAGCATGG NGG 2 0.2424 Tier II
26 NC_000007.14 7 47277426 + TNS3 NNNCAGAGCACTCACCATGG NGG 2 0.2338 Tier II
27 NC_000011.10 11 72649210 - PDE2A NNNCAGAGCTGACAGCATGG NGG 2 0.2 Tier II
28 NC_000002.12 2 196822309 - C2orf66 NNNCAGTGCTCTCAGCATGC NGG 2 0.1875 Tier II
29 NC_000007.14 7 103562756 + RELN NNNCACAGCTGTCAGCATGG NGG 2 0.1705 Tier II
30 NC_000010.11 10 131178138 - TCERG1L NNNCAGAGCGCTCAGCAGGG NGG 2 0.1667 Tier II
31 NC_000015.10 15 76148985 - TMEM266 NNNCAGAACTCTCAGGATGG NGG 2 0.1538 Tier II
32 NC_000018.10 18 63709217 - SERPINB11 NNNCAGAGATCTCAGCCTGG NGG 2 0.1513 Tier II
33 NC_000005.10 5 149378492 + IL17B NNNCAGAGCTCCCAGGATGG NGG 2 0.1457 Tier II
34 NC_000005.10 5 139587014 + UBE2D2 NNNCAGAGCTCTCAAGATGG NGG 2 0.1448 Tier II
35 NC_000006.12 6 29558953 - UBD NNNCAGAGCTCTCAAGATGG NGG 2 0.1448 Tier II
36 NC_000017.11 17 38504465 - ARHGAP23 NNNCAGAGCTCACAGCCTGG NGG 2 0.1412 Tier II
37 NC_000011.10 11 48074882 + PTPRJ NNNCAGTGCTCTCAGCATGG NAG 2 0.1134 Tier II
38 NC_000002.12 2 26568047 - CIMIP2C NNNCAGACCTCTCAGCCTGG NGG 2 0.1086 Tier II
39 NC_000007.14 7 73557077 + BCL7B NNNCAGAGCGCTCAGCCTGG NGG 2 0.0882 Tier II
40 NC_000014.9 14 23550926 - THTPA NNNCAGAGCTGTCAGCCTGG NGG 2 0.0441 Tier II
41 NC_000014.9 14 23550926 - ZFHX2 NNNCAGAGCTGTCAGCCTGG NGG 2 0.0441 Tier II
42 NC_000003.12 3 133881188 - RAB6B NNNCAGAGCTCGCAGCATGG NGA 2 0.0347 Tier II
43 NC_000004.12 4 158162314 - GASK1B NNNCAGAGCTCTCAGCTTGG NAG 2 0.0346 Tier II
44 NC_000020.11 20 59309282 - EDN3 NNNCAGAGCTCTCAGCATAG NTG 2 0.0278 Tier II
45 NC_000002.12 2 236130945 + AGAP1 NNNCAGGGCTCTCAGCATGG NTG 2 0.0275 Tier II
46 NC_000003.12 3 61113532 - FHIT NNNCAAAGCTCTCAGCATGG NGC 2 0.0222 Tier II
47 NC_000007.14 7 32417422 - PDE1C NNNCAAAGCTCTCAGCATGG NGC 2 0.0222 Tier II
48 NC_000011.10 11 67583896 + GSTP1 NNNCAGAGCTCCCAGCATGG NGC 2 0.0211 Tier II
49 NC_000002.12 2 3613422 + COLEC11 NNNCAGAGCTCTGAGGATGG NGG 2 0.021 Tier II
50 NC_000022.11 22 17855014 - MICAL3 NNNCAGAGCTCTCAGGTTGG NGG 2 0.0205 Tier II
51 NC_000002.12 2 9314526 - ASAP2 NNNCAGAGCTCTTAGCATGG NGC 2 0.0156 Tier II
52 NC_000002.12 2 109180906 - RANBP2 NNNCAGAGCTCTCTGCATGG NGT 2 0.0086 Tier II
53 NC_000002.12 2 109180906 - SH3RF3 NNNCAGAGCTCTCTGCATGG NGT 2 0.0086 Tier II
54 NC_000004.12 4 114967475 + NDST4 NNNCAGAGCTCTCAGCATGG NTC 2 0.0 Tier II
55 NC_000009.12 9 133222172 + OBP2B NNNCAGAGCTCTCAGCATGG NTC 2 0.0 Tier II
56 NC_000015.10 15 22883041 + CYFIP1 NNNCAGAGCTCTCAGCATGG NCC 2 0.0 Tier II
57 NC_000001.11 1 228942860 - LOC124904539 NNNCAGAGCTCCCAACATGG NGG 2 0.8916 Tier III
58 NC_000001.11 1 228954261 - LOC105373143 NNNCAGAGCTCCCAACATGG NGG 2 0.8916 Tier III
59 NC_000009.12 9 36265357 - LOC124902150 NNNCAGAGCTCCCAACATGG NGG 2 0.8916 Tier III
60 NC_000001.11 1 209585272 - LOC107985256 NNNCAGATTTCTCAGCATGG NGG 2 0.5469 Tier III
61 NC_000010.11 10 121076338 + LOC105378521 NNNCAGGGCTCTCAGCATTG NGG 2 0.4706 Tier III
62 NC_000009.12 9 93808515 + LOC101928014 NNNCAGAGCTCTCAGCATCA NGG 2 0.4203 Tier III
63 NC_000017.11 17 83207425 + LOC101929650 NNNGGGAGCTCTCAGCATGG NGG 2 0.36 Tier III
64 NC_000005.10 5 173707749 + LINC01484 NNNCAGAGCTCTCAGCATGG NAG 1 0.2593 Tier III
65 NC_000001.11 1 207804971 - MIR29B2CHG NNNCAGAGCTCTCAGCAGGC NGG 2 0.1429 Tier III
66 NC_000002.12 2 164848335 - LOC101929633 NNNCAGAGCTCTCAGCTTGG NGG 1 0.1333 Tier III
67 NC_000023.11 X 132984747 - LOC124905219 NNNCAGAGCTCACAGGATGG NGG 2 0.1231 Tier III
68 NC_000008.11 8 126867220 + LOC105375751 NNNCACAGCTCTCAGCATGG NCG 2 0.0731 Tier III
69 NC_000022.11 22 49047134 + NHIP NNNCTGAGCTCTCAGCTTGG NGG 2 0.0485 Tier III
70 NC_000001.11 1 29503849 + LOC107984933 NNNCAGAGCTCTCAGCCTGG NGA 2 0.0123 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 5488267 + Vmn2r28 NNNCAGAAATCTCAGCATGG NGG 2 0.8571 Tier I
2 NC_000069.6 3 55477816 + Dclk1 NNNCAGATCTCTCAGCATGG NGG 1 0.625 Tier I
3 NC_000081.6 15 78483952 - Il2rb NNNCTGAGCTCCCAGCATGG NGG 2 0.3445 Tier I
4 NC_000075.6 9 30921688 + Adamts15 NNNCAGAGCCCTCAGGATGG NGG 2 0.0821 Tier I
5 NC_000071.6 5 124741940 - Dnah10 NNNCAGAGCGCTCATCATGG NGG 2 0.0714 Tier I
6 NC_000068.7 2 69897296 + Ubr3 NNNCTGAGCTCTCATCATGG NGG 2 0.0519 Tier I
7 NC_000078.6 12 108675560 - Evl NNNCAGAGCTCTCATCATGG NTG 2 0.0056 Tier I
8 NC_000078.6 12 74025561 - Syt16 NNNCAGAGCTCTCAACATGG NGG 1 0.9412 Tier II
9 NC_000083.6 17 49871510 + Kif6 NNNCAGAGCTCTCAGAATGA NGG 2 0.9375 Tier II
10 NC_000074.6 8 120440152 + Gse1 NNNCAGAGTTCCCAGCATGG NGG 2 0.8289 Tier II
11 NC_000070.6 4 156185780 - Agrn NNNCAGAGCACCCAGCATGG NGG 2 0.812 Tier II
12 NC_000078.6 12 4951620 - Atad2b NNNTAGAACTCTCAGCATGG NGG 2 0.8 Tier II
13 NC_000077.6 11 33244099 + Ranbp17 NNNAAGAGCTATCAGCATGG NGG 2 0.6316 Tier II
14 NC_000085.6 19 40723877 + Entpd1 NNNCAGAGCTCCCAGCATTG NGG 2 0.6316 Tier II
15 NC_000078.6 12 80579049 + Galnt16 NNNCAGAGGTCTCAGCATGG NGG 1 0.619 Tier II
16 NC_000071.6 5 101872362 + Wdfy3 NNNCAGAGCTAACAGCATGG NGG 2 0.6 Tier II
17 NC_000067.6 1 56855407 + Satb2 NNNCAGAGCTCTCTGCATGG NGG 1 0.5333 Tier II
18 NC_000072.6 6 108066673 - Setmar NNNAAGATCTCTCAGCATGG NGG 2 0.5263 Tier II
19 NC_000078.6 12 83322003 + Dpf3 NNNCATGGCTCTCAGCATGG NGG 2 0.4706 Tier II
20 NC_000073.6 7 136933963 - Mgmt NNNCAGAGACCTCAGCATGG NGG 2 0.4571 Tier II
21 NC_000076.6 10 59766667 - Micu1 NNNCAGTGCTCTCAGCATGG NGG 1 0.4375 Tier II
22 NC_000079.6 13 19117785 + Amph NNNCAGAGCACGCAGCATGG NGG 2 0.4286 Tier II
23 NC_000077.6 11 118908628 - Rbfox3 NNNCAGAGCCCTCAGCATAG NGG 2 0.381 Tier II
24 NC_000083.6 17 44516687 - Runx2 NNNCAGAGATCTCAGCATGC NGG 2 0.3673 Tier II
25 NC_000083.6 17 23838192 + Prss41 NNNCTGAACTCTCAGCATGG NGG 2 0.3636 Tier II
26 NC_000069.6 3 56004029 - Nbea NNNCCGAGCTCTCAGCATTG NGG 2 0.3333 Tier II
27 NC_000067.6 1 120181504 - 3110009E18Rik NNNCAGAGGTCTCTGCATGG NGG 2 0.3302 Tier II
28 NC_000078.6 12 36535191 + Crppa NNNCTGAGTTCTCAGCATGG NGG 2 0.3182 Tier II
29 NC_000070.6 4 115835525 - Mob3c NNNCAGAGGGCTCAGCATGG NGG 2 0.3095 Tier II
30 NC_000075.6 9 32466861 + Fli1 NNNCAGAGCTCTCAGCAAGC NGG 2 0.2857 Tier II
31 NC_000071.6 5 125119741 - Ncor2 NNNCAGGGCTCTAAGCATGG NGG 2 0.2715 Tier II
32 NC_000073.6 7 49927461 - Slc6a5 NNNCAGAGCTCTAAGCATTG NGG 2 0.2564 Tier II
33 NC_000086.7 X 74276779 + Dnase1l1 NNNCAGAGCTGTCAACATGG NGG 2 0.2353 Tier II
34 NC_000071.6 5 125432010 - Dhx37 NNNCAGGGCTCTCAGCATGG NAG 2 0.183 Tier II
35 NC_000073.6 7 91092175 + Dlg2 NNNCAGACCTCTCAGCATGG NAG 2 0.1595 Tier II
36 NC_000069.6 3 13975722 + Ralyl NNNCAGAGCTCCCAGCTTGG NGG 2 0.1263 Tier II
37 NC_000077.6 11 24077408 + Bcl11a NNNCAGAGCTCTCAACTTGG NGG 2 0.1255 Tier II
38 NC_000086.7 X 162103925 - Nhs NNNCAGAGCTCTTAGCCTGG NGG 2 0.1235 Tier II
39 NC_000067.6 1 124557780 - Dpp10 NNNCAGAGATCTGAGCATGG NGG 2 0.1169 Tier II
40 NC_000081.6 15 28298017 - Dnah5 NNNCAGAGCTCTCAGGAAGG NGG 2 0.1026 Tier II
41 NC_000074.6 8 83237288 - Tbc1d9 NNNCAGGGCTCTGAGCATGG NGG 2 0.0963 Tier II
42 NC_000079.6 13 38405463 + Bmp6 NNNCAGAGCTTTCAGTATGG NGG 2 0.0947 Tier II
43 NC_000078.6 12 102265851 + Slc24a4 NNNCAGAGCTCTCTGCTTGG NGG 2 0.0711 Tier II
44 NC_000081.6 15 64884960 - Adcy8 NNNCTGAGCTCTCAGCCTGG NGG 2 0.0642 Tier II
45 NC_000081.6 15 21301422 + Cdh12 NNNCAGAGCTCTGGGCATGG NGG 2 0.0636 Tier II
46 NC_000073.6 7 82758616 + Efl1 NNNCAGAGTTCTCAGCATGG NGA 2 0.0608 Tier II
47 NC_000070.6 4 137729031 + Rap1gap NNNCAGAGCTCTCAGAATGG NTG 2 0.039 Tier II
48 NC_000069.6 3 125576026 - Ndst4 NNNCAGAGCTCTCAGCATGG NTG 1 0.039 Tier II
49 NC_000078.6 12 116535645 - Ptprn2 NNNCAGAGCACTCAGCATGG NTG 2 0.0334 Tier II
50 NC_000078.6 12 41048895 + Immp2l NNNCAGAGCTCTCTGCATGG NTG 2 0.0208 Tier II
51 NC_000067.6 1 39440454 + Tbc1d8 NNNCAGAGCTCTCAGCATGC NGC 2 0.0095 Tier II
52 NC_000084.6 18 69602536 + Tcf4 NNNCAGAGCTCTCAGCATGC NGC 2 0.0095 Tier II
53 NC_000084.6 18 15092560 - Kctd1 NNNCTGAGCTCTCAGCATGG NGC 2 0.0081 Tier II
54 NC_000077.6 11 118514629 + Rbfox3 NNNCAGAGCTCTCAGCCTGG NTG 2 0.0069 Tier II
55 NC_000084.6 18 13811626 - Zfp521 NNNCAGAGCTCTCAGGATGG NGT 2 0.0025 Tier II
56 NC_000073.6 7 7714589 + Vmn2r-ps38 NNNCAGAAATCTCAGCATGG NGG 2 0.8571 Tier III
57 NC_000073.6 7 8514207 + Vmn2r-ps47 NNNCAGAAATCTCAGCATGG NGG 2 0.8571 Tier III
58 NC_000073.6 7 9248807 + Gm4011 NNNCAGAAATCTCAGCATGG NGG 2 0.8571 Tier III
59 NC_000073.6 7 19752607 - Gm34744 NNNCAAGGCTCTCAGCATGG NGG 2 0.7059 Tier III
60 NC_000081.6 15 74302329 + Gm19859 NNNCAGGGCTCCCAGCATGG NGG 2 0.6687 Tier III
61 NC_000069.6 3 55477816 + Gm31443 NNNCAGATCTCTCAGCATGG NGG 1 0.625 Tier III
62 NC_000069.6 3 56004029 - Gm31914 NNNCCGAGCTCTCAGCATTG NGG 2 0.3333 Tier III
63 NC_000067.6 1 102264758 + Gm3116 NNNCAGAGCTCCCAGTATGG NGG 2 0.2915 Tier III
64 NC_000085.6 19 43642040 + Gm34922 NNNCAGAGCTCTCAGGAAGG NGG 2 0.1026 Tier III
65 NC_000069.6 3 38681251 - Gm29879 NNNCAGAGCTCTGAGCAAGG NGG 2 0.0909 Tier III
66 NC_000082.6 16 49767711 + Gm15518 NNNCAGAGCTCCCAGCATGG NTG 2 0.0369 Tier III
67 NC_000076.6 10 61829682 + Gm51765 NNNCAGAGCTCTCTGCATGG NTG 2 0.0208 Tier III
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Other clones with same target sequence:

(none)