Construct: sgRNA BRDN0001147488
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGCCCCTGAAGACATACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA2 (1969)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76369
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
16133583 |
- |
EPHA2 |
NNNGCCCCTGAAGACATACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
37998972 |
+ |
FHL3 |
NNNGCCCCTGAACACATACC |
NGG |
2 |
0.1805 |
Tier I |
| 3 |
NC_000001.11 |
1 |
6266352 |
+ |
ACOT7 |
NNNTCCCCTGAAGACATACA |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000023.11 |
X |
48998794 |
- |
GRIPAP1 |
NNNGCCCCTCAAGACATTCG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000013.11 |
13 |
110406937 |
- |
COL4A2 |
NNNGCCCCTGAAGGCATAAG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000002.12 |
2 |
130883664 |
- |
ARHGEF4 |
NNNGCCCCTGCAGACATGCG |
NGG |
2 |
0.16 |
Tier II |
| 7 |
NC_000016.10 |
16 |
70162343 |
+ |
PDPR |
NNNGGCCCTGAAGACATCCG |
NGG |
2 |
0.1143 |
Tier II |
| 8 |
NC_000014.9 |
14 |
99581384 |
+ |
CCDC85C |
NNNGCCCCTGAAGACAACCG |
NGG |
2 |
0.1016 |
Tier II |
| 9 |
NC_000005.10 |
5 |
122947488 |
+ |
SNX24 |
NNNGCCCCTGAAGACATCTG |
NGG |
2 |
0.0879 |
Tier II |
| 10 |
NC_000005.10 |
5 |
122339875 |
- |
SNCAIP |
NNNGCCCCTGAAGACAGACT |
NGG |
2 |
0.0824 |
Tier II |
| 11 |
NC_000011.10 |
11 |
121476094 |
+ |
SORL1 |
NNNGCCCCTGAAGATATACG |
NGG |
1 |
0.0667 |
Tier II |
| 12 |
NC_000017.11 |
17 |
75764269 |
- |
GALK1 |
NNNGCCCCTGAAGACCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000016.10 |
16 |
70162343 |
+ |
LOC400541 |
NNNGGCCCTGAAGACATCCG |
NGG |
2 |
0.1143 |
Tier III |
| 14 |
NC_000011.10 |
11 |
121476094 |
+ |
LOC105369535 |
NNNGCCCCTGAAGATATACG |
NGG |
1 |
0.0667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
130100582 |
- |
AU015228 |
NNNGCCCCTGACGACATACT |
NGG |
2 |
0.1842 |
Tier I |
| 2 |
NC_000073.6 |
7 |
25615726 |
+ |
Erich4 |
NNNGCCCCTGAAGACATAAG |
NGC |
2 |
0.0095 |
Tier I |
| 3 |
NC_000070.6 |
4 |
141321489 |
+ |
Epha2 |
NNNGCCCCTGAAGACCTATG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000078.6 |
12 |
69982095 |
+ |
Sav1 |
NNNGCCCGTGAAGACATACA |
NGG |
2 |
0.6027 |
Tier II |
| 5 |
NC_000071.6 |
5 |
107634306 |
+ |
Rpap2 |
NNNGTCACTGAAGACATACG |
NGG |
2 |
0.4773 |
Tier II |
| 6 |
NC_000068.7 |
2 |
18695551 |
+ |
Spag6 |
NNNGACCCTTAAGACATACG |
NGG |
2 |
0.4643 |
Tier II |
| 7 |
NC_000074.6 |
8 |
116584709 |
+ |
Cdyl2 |
NNNGCCCTTGAAGAAATACG |
NGG |
2 |
0.1944 |
Tier II |
| 8 |
NC_000076.6 |
10 |
127886207 |
- |
Rdh7 |
NNNGCCCCTGAAGACATCTG |
NGG |
2 |
0.0879 |
Tier II |
| 9 |
NC_000079.6 |
13 |
55349826 |
+ |
Lman2 |
NNNACCCCTGAAGACATACG |
NGA |
2 |
0.0625 |
Tier II |
| 10 |
NC_000078.6 |
12 |
69982095 |
+ |
Gm19221 |
NNNGCCCGTGAAGACATACA |
NGG |
2 |
0.6027 |
Tier III |
| 11 |
NC_000073.6 |
7 |
78604375 |
- |
E430016F16Rik |
NNNTCCCCTGAAGACATACA |
NGG |
2 |
0.3409 |
Tier III |
| 12 |
NC_000079.6 |
13 |
69688244 |
+ |
Gm35618 |
NNNGCCCCTGTAGACATTCG |
NGG |
2 |
0.1538 |
Tier III |
| 13 |
NC_000079.6 |
13 |
69688244 |
+ |
Gm40993 |
NNNGCCCCTGTAGACATTCG |
NGG |
2 |
0.1538 |
Tier III |
Other clones with same target sequence:
(none)
