Construct: sgRNA BRDN0001147492
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGAAATCTACTTGATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSF1R (1436)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77037
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150077368 |
+ |
CSF1R |
NNNGGAAATCTACTTGATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
36737372 |
- |
DCLK3 |
NNNGGAAATCTACCTGATCC |
NGG |
2 |
0.1224 |
Tier I |
| 3 |
NC_000017.11 |
17 |
5322691 |
- |
RABEP1 |
NNNAGTAATCTACTTGATCG |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000007.14 |
7 |
159073300 |
- |
VIPR2 |
NNNGAAAATCTACTTGATTG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000002.12 |
2 |
124845338 |
+ |
CNTNAP5 |
NNNTGAAATCTACTTGATCA |
NGG |
2 |
0.3409 |
Tier II |
| 6 |
NC_000023.11 |
X |
110766996 |
- |
CHRDL1 |
NNNTGAAACCTACTTGATCG |
NGG |
2 |
0.3357 |
Tier II |
| 7 |
NC_000012.12 |
12 |
9188180 |
- |
PZP |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000012.12 |
12 |
9188180 |
- |
KLRG1 |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier II |
| 9 |
NC_000023.11 |
X |
29539217 |
- |
IL1RAPL1 |
NNNTGAAATCTACTTGATCT |
NGG |
2 |
0.2545 |
Tier II |
| 10 |
NC_000003.12 |
3 |
7336199 |
- |
GRM7 |
NNNTGAAATCCACTTGATCG |
NGG |
2 |
0.2424 |
Tier II |
| 11 |
NC_000004.12 |
4 |
172260961 |
+ |
GALNTL6 |
NNNTGAAATCCACTTGATCG |
NGG |
2 |
0.2424 |
Tier II |
| 12 |
NC_000010.11 |
10 |
23022123 |
- |
ARMC3 |
NNNGGAAATCTTCTTGATCT |
NGG |
2 |
0.2333 |
Tier II |
| 13 |
NC_000004.12 |
4 |
8110647 |
+ |
ABLIM2 |
NNNGGAAATATACTTGGTCG |
NGG |
2 |
0.1529 |
Tier II |
| 14 |
NC_000002.12 |
2 |
47344438 |
- |
EPCAM-DT |
NNNGGAAATCCACTAGATCG |
NGG |
2 |
0.386 |
Tier III |
| 15 |
NC_000023.11 |
X |
149872670 |
- |
LINC00850 |
NNNTGAAACCTACTTGATCG |
NGG |
2 |
0.3357 |
Tier III |
| 16 |
NC_000008.11 |
8 |
92763861 |
- |
FLJ46284 |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier III |
| 17 |
NC_000010.11 |
10 |
23022123 |
- |
LOC107984215 |
NNNGGAAATCTTCTTGATCT |
NGG |
2 |
0.2333 |
Tier III |
| 18 |
NC_000012.12 |
12 |
82229667 |
+ |
LOC105369873 |
NNNGGAAATCTACTTTATCT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
49339589 |
- |
Grik2 |
NNNGGAAATCTACTTGATCA |
NGG |
1 |
0.9375 |
Tier II |
| 2 |
NC_000067.6 |
1 |
150883321 |
- |
Hmcn1 |
NNNGGAAGCCTACTTGATCG |
NGG |
2 |
0.6769 |
Tier II |
| 3 |
NC_000069.6 |
3 |
57348118 |
+ |
Tm4sf1 |
NNNGGAAGCCTACTTGATCG |
NGG |
2 |
0.6769 |
Tier II |
| 4 |
NC_000076.6 |
10 |
53101693 |
+ |
Slc35f1 |
NNNGGAAATCTACATGAACG |
NGG |
2 |
0.4127 |
Tier II |
| 5 |
NC_000085.6 |
19 |
43252929 |
+ |
Hpse2 |
NNNTGAAATCTACTTGATCA |
NGG |
2 |
0.3409 |
Tier II |
| 6 |
NC_000068.7 |
2 |
11901761 |
+ |
Gm34768 |
NNNTGAAACCTACTTGATCG |
NGG |
2 |
0.3357 |
Tier II |
| 7 |
NC_000083.6 |
17 |
80588250 |
+ |
Map4k3 |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000084.6 |
18 |
25208183 |
+ |
AW554918 |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier II |
| 9 |
NC_000072.6 |
6 |
96897512 |
- |
Tafa4 |
NNNTGAAATCTACTTGATTG |
NGG |
2 |
0.1678 |
Tier II |
| 10 |
NC_000086.7 |
X |
59349722 |
+ |
Fgf13 |
NNNTGAAATCTACTTGCTCG |
NGG |
2 |
0.0642 |
Tier II |
| 11 |
NC_000080.6 |
14 |
99183181 |
+ |
Pibf1 |
NNNGGAAATCTACTTGATTG |
NGA |
2 |
0.0321 |
Tier II |
| 12 |
NC_000068.7 |
2 |
17005699 |
- |
4930515L03Rik |
NNNTGAAATCTACTTGATCA |
NGG |
2 |
0.3409 |
Tier III |
| 13 |
NC_000067.6 |
1 |
17113693 |
+ |
Gm31982 |
NNNTGAAACCTACTTGATCG |
NGG |
2 |
0.3357 |
Tier III |
| 14 |
NC_000070.6 |
4 |
37260979 |
+ |
Gm12374 |
NNNTGAAACCTACTTGATCG |
NGG |
2 |
0.3357 |
Tier III |
| 15 |
NC_000073.6 |
7 |
105833920 |
- |
9130208D14Rik |
NNNTGAAGTCTACTTGATCG |
NGG |
2 |
0.2667 |
Tier III |
| 16 |
NC_000084.6 |
18 |
26277401 |
- |
Gm41679 |
NNNGGAAATCTACTGGATGG |
NGG |
2 |
0.0063 |
Tier III |
Other clones with same target sequence:
(none)
