Construct: sgRNA BRDN0001147494

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCCTCTTGCTGACGTCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK18 (5129)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77879

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 205524232 - CDK18 NNNCTCTTGCTGACGTCCTG NGG 0 1.0 Tier I
2 NC_000010.11 10 70083424 + MACROH2A2 NNNCTCCTGCTGATGTCCTG NGG 2 0.5042 Tier II
3 NC_000019.10 19 2734111 - SLC39A3 NNNCTCTTGCAGACGCCCTG NGG 2 0.5 Tier II
4 NC_000007.14 7 123633091 + ASB15 NNNCTCTTGCTGACGTCCAA NGG 2 0.2679 Tier II
5 NC_000003.12 3 112047413 + TMPRSS7 NNNCTCTTGCAGAAGTCCTG NGG 2 0.2625 Tier II
6 NC_000011.10 11 45149144 - PRDM11 NNNCTCTTGCTGTTGTCCTG NGG 2 0.22 Tier II
7 NC_000005.10 5 135601389 + SLC25A48 NNNCTCTTGCTGCCATCCTG NGG 2 0.1981 Tier II
8 NC_000003.12 3 123693206 - MYLK NNNCTCTTGGTGACGTCCAG NGG 2 0.1111 Tier II
9 NC_000013.11 13 107212497 - NALF1 NNNCTCTTGCTGTCCTCCTG NGG 2 0.0818 Tier II
10 NC_000002.12 2 231521922 + NMUR1 NNNCTGTTGCTGACGTGCTG NGG 2 0.0294 Tier II
11 NC_000022.11 22 21653861 - LOC107985532 NNNCTCCAGCTGACGTCCTG NGG 2 0.55 Tier III
12 NC_000018.10 18 11464532 - LINC01928 NNNCTTTTGCTGCCGTCCTG NGG 2 0.1955 Tier III
13 NC_000018.10 18 11464532 - LOC107985173 NNNCTTTTGCTGCCGTCCTG NGG 2 0.1955 Tier III
14 NC_000003.12 3 123693206 - LOC105369194 NNNCTCTTGGTGACGTCCAG NGG 2 0.1111 Tier III
15 NC_000012.12 12 6158511 - LOC124902866 NNNCTCTTGCTGACCTCCAG NGG 2 0.0779 Tier III
16 NC_000020.11 20 49957472 - KRT18P4 NNNATCTTGCTGACGTCCTG NGA 2 0.0585 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 55494153 - Adcyap1r1 NNNCTCTTGCTGACGTTCTC NGG 2 0.2 Tier I
2 NC_000067.6 1 132121574 + Cdk18 NNNCTCTTGTTGAGGTCCTG NGG 2 0.0 Tier I
3 NC_000068.7 2 158795188 + Dhx35 NNNATCTTGCTGAAGTCCTG NGG 2 0.2947 Tier II
4 NC_000070.6 4 104532713 + Dab1 NNNCTGTTTCTGACGTCCTG NGG 2 0.2667 Tier II
5 NC_000084.6 18 38278879 - Pcdh12 NNNCTCTGGCTGACTTCCTG NGG 2 0.1048 Tier II
6 NC_000084.6 18 38278879 - Rnf14 NNNCTCTGGCTGACTTCCTG NGG 2 0.1048 Tier II
7 NC_000071.6 5 89143931 + Slc4a4 NNNCTCTTGCTGATGTCCTG NGC 2 0.0163 Tier II
8 NC_000079.6 13 119750625 + Nim1k NNNCTCTTGCTGACGTCCTG NGT 1 0.0161 Tier II
9 NC_000082.6 16 11225370 - Gspt1 NNNCTCTTGCTGACGACCTG NGT 2 0.0147 Tier II
10 NC_000075.6 9 88431391 - Snx14 NNNCTCTTGCAGACGTCCTG NGT 2 0.0121 Tier II
11 NC_000082.6 16 35565393 + Sema5b NNNCTCTTGCTGAAGTCCTG NGT 2 0.0056 Tier II
12 NC_000076.6 10 24682283 + Enpp1 NNNCTCTTCCTGAGGTCCTG NGG 2 0.0 Tier II
13 NC_000069.6 3 90116673 - Nup210l NNNCTCTTGCTGAGGACCTG NGG 2 0.0 Tier II
14 NC_000069.6 3 51534903 + Setd7 NNNCTCTTGCTGAGGTCCTG NGT 2 0.0 Tier II
15 NC_000070.6 4 134214647 - Catsper4 NNNCTCTTGCTGAGGTCCTG NGT 2 0.0 Tier II
16 NC_000073.6 7 45746789 - Sult2b1 NNNCTCTTGCTGAGGTCCTG NGT 2 0.0 Tier II
17 NC_000086.7 X 60153634 + Mcf2 NNNCTCTTGCTGAGGTCCTG NGT 2 0.0 Tier II
18 NC_000082.6 16 11225370 - 2610020C07Rik NNNCTCTTGCTGACGACCTG NGT 2 0.0147 Tier III
19 NC_000073.6 7 45746789 - Gm31597 NNNCTCTTGCTGAGGTCCTG NGT 2 0.0 Tier III
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Other clones with same target sequence:

(none)