Construct: sgRNA BRDN0001147502
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATAATAGGGAGTATAGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK3 (7867)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77240
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
50644514 |
- |
MAPKAPK3 |
NNNTAATAGGGAGTATAGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
21395155 |
+ |
CHD8 |
NNNGAATAGGAAGTATAGCA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000003.12 |
3 |
197699898 |
- |
RUBCN |
NNNTAAGAGGAAGTATAGCA |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000001.11 |
1 |
2488674 |
- |
PLCH2 |
NNNGAATGGGGAGTATAGCA |
NGG |
2 |
0.4583 |
Tier II |
| 5 |
NC_000010.11 |
10 |
24171334 |
+ |
KIAA1217 |
NNNAAATAGGGAATATAGCA |
NGG |
2 |
0.4396 |
Tier II |
| 6 |
NC_000017.11 |
17 |
36308504 |
- |
LOC101060212 |
NNNTAATAGGGAGAATAACA |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000017.11 |
17 |
47580587 |
- |
NPEPPS |
NNNTAATAGGGAGAATAACA |
NGG |
2 |
0.4286 |
Tier II |
| 8 |
NC_000002.12 |
2 |
134602952 |
- |
TMEM163 |
NNNCAATAGGGAGTATAGCT |
NGG |
2 |
0.3882 |
Tier II |
| 9 |
NC_000022.11 |
22 |
46085337 |
+ |
LOC124905135 |
NNNTAATAGGGAACATAGCA |
NGG |
2 |
0.2637 |
Tier II |
| 10 |
NC_000001.11 |
1 |
49543333 |
- |
AGBL4 |
NNNTAATAAGGAGTAGAGCA |
NGG |
2 |
0.2225 |
Tier II |
| 11 |
NC_000003.12 |
3 |
192652312 |
+ |
FGF12 |
NNNCAATAGGGAGCATAGCA |
NGG |
2 |
0.1849 |
Tier II |
| 12 |
NC_000018.10 |
18 |
26952991 |
- |
CHST9 |
NNNTAAAAGGGAGTATAGCA |
NTG |
2 |
0.0341 |
Tier II |
| 13 |
NC_000010.11 |
10 |
73501365 |
- |
USP54 |
NNNTAATAGGGAGTAGAGCA |
NGA |
2 |
0.024 |
Tier II |
| 14 |
NC_000002.12 |
2 |
85404313 |
- |
CAPG |
NNNTAATAGGGTGTATAGCA |
NTG |
2 |
0.013 |
Tier II |
| 15 |
NC_000018.10 |
18 |
31679794 |
- |
B4GALT6 |
NNNTAATAGGGAGTATAGAA |
NGT |
2 |
0.0069 |
Tier II |
| 16 |
NC_000017.11 |
17 |
38112608 |
- |
LOC101929950 |
NNNTAATAGGGAGAATAACA |
NGG |
2 |
0.4286 |
Tier III |
| 17 |
NC_000017.11 |
17 |
38207914 |
+ |
NPEPPSP1 |
NNNTAATAGGGAGAATAACA |
NGG |
2 |
0.4286 |
Tier III |
| 18 |
NC_000008.11 |
8 |
117184155 |
+ |
LOC105375716 |
NNNTAATAGGGAGTGTAGCA |
NGA |
2 |
0.0451 |
Tier III |
| 19 |
NC_000010.11 |
10 |
73501365 |
- |
PPP3CB-AS1 |
NNNTAATAGGGAGTAGAGCA |
NGA |
2 |
0.024 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
107260064 |
+ |
Mapkapk3 |
NNNTAATAGGGAGTGTAGCA |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000084.6 |
18 |
43245979 |
+ |
Stk32a |
NNNCAATAGAGAGTATAGCA |
NGG |
2 |
0.6039 |
Tier II |
| 3 |
NC_000082.6 |
16 |
7345086 |
+ |
Rbfox1 |
NNNCAATACGGAGTATAGCA |
NGG |
2 |
0.3484 |
Tier II |
| 4 |
NC_000085.6 |
19 |
23604297 |
+ |
1700028P14Rik |
NNNAAGTAGGGAGTATAGCA |
NGG |
2 |
0.3401 |
Tier II |
| 5 |
NC_000067.6 |
1 |
10297140 |
- |
Cpa6 |
NNNTAAAAGGGAGCATAGCA |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000079.6 |
13 |
8275897 |
- |
Adarb2 |
NNNAACTAGGGAGTATAGCA |
NGG |
2 |
0.2165 |
Tier II |
| 7 |
NC_000072.6 |
6 |
127984639 |
+ |
Tspan9 |
NNNTAATAGGGTGAATAGCA |
NGG |
2 |
0.2063 |
Tier II |
| 8 |
NC_000083.6 |
17 |
19661481 |
+ |
Vmn2r102 |
NNNTAATAGGAAGTATGGCA |
NGG |
2 |
0.1765 |
Tier II |
| 9 |
NC_000081.6 |
15 |
48606671 |
+ |
Csmd3 |
NNNTAATAGGGAGTAAAGCA |
NTG |
2 |
0.0354 |
Tier II |
| 10 |
NC_000072.6 |
6 |
85217236 |
- |
Sfxn5 |
NNNTAATAGGGAGTGTAGCA |
NTG |
2 |
0.0253 |
Tier II |
| 11 |
NC_000085.6 |
19 |
36435823 |
+ |
Pcgf5 |
NNNTAATAGGGAGTTTAGGA |
NGG |
2 |
0.025 |
Tier II |
Other clones with same target sequence:
(none)
