Construct: sgRNA BRDN0001147508
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCAATGTGATGCTCGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PXK (54899)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78020
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
58397106 |
+ |
PXK |
NNNCAATGTGATGCTCGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
34959595 |
- |
CCT6B |
NNNCAATGTGCTGCTCGATG |
NGA |
2 |
0.0278 |
Tier I |
| 3 |
NC_000019.10 |
19 |
53900640 |
+ |
PRKCG |
NNNCAATGTGATGCTGGATG |
NTG |
2 |
0.006 |
Tier I |
| 4 |
NC_000009.12 |
9 |
5847258 |
+ |
ERMP1 |
NNNGATTGTGATGCTCGATG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000004.12 |
4 |
21151650 |
- |
KCNIP4 |
NNNCAATGTGATGATAGATG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000006.12 |
6 |
128218207 |
+ |
PTPRK |
NNNCAATATGATGCTCCATG |
NGG |
2 |
0.2353 |
Tier II |
| 7 |
NC_000016.10 |
16 |
64994595 |
- |
CDH11 |
NNNCAGTGTGATGCTGGATG |
NGG |
2 |
0.1099 |
Tier II |
| 8 |
NC_000017.11 |
17 |
4448892 |
+ |
SPNS3 |
NNNGAATGTGATGCTGGATG |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000001.11 |
1 |
64077937 |
+ |
ROR1 |
NNNCAATGTGATGCTCAATG |
NGA |
2 |
0.0648 |
Tier II |
| 10 |
NC_000011.10 |
11 |
19569777 |
+ |
NAV2 |
NNNCAATGTGATGCTCCCTG |
NGG |
2 |
0.0448 |
Tier II |
| 11 |
NC_000002.12 |
2 |
229093842 |
- |
PID1 |
NNNCAATGTGATGCTGGCTG |
NGG |
2 |
0.0293 |
Tier II |
| 12 |
NC_000019.10 |
19 |
53566213 |
- |
ZNF331 |
NNNCAATGTGATGCTGGATG |
NGC |
2 |
0.0034 |
Tier II |
| 13 |
NC_000018.10 |
18 |
23966915 |
- |
LINC02958 |
NNNCACTGTGATGCTCGGTG |
NGG |
2 |
0.1818 |
Tier III |
| 14 |
NC_000014.9 |
14 |
105533330 |
- |
LOC105370697 |
NNNCAATGTGATGCTCGCTG |
NTG |
2 |
0.0074 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
7967657 |
+ |
Wdr70 |
NNNAAATGTGATGCTCGATA |
NGG |
2 |
0.7895 |
Tier II |
| 2 |
NC_000068.7 |
2 |
168516814 |
- |
Nfatc2 |
NNNCAATGTGACGCTCGATG |
NGA |
2 |
0.0658 |
Tier II |
| 3 |
NC_000074.6 |
8 |
91534797 |
- |
Fto |
NNNCTATGTGATGCTGGATG |
NGG |
2 |
0.0559 |
Tier II |
| 4 |
NC_000085.6 |
19 |
11273595 |
- |
4930526L06Rik |
NNNCACTGTGATGCTTGATG |
NGG |
2 |
0.1399 |
Tier III |
| 5 |
NC_000077.6 |
11 |
62993940 |
+ |
Cdrt4os1 |
NNNCAGTGTGATGCTCGCTG |
NGG |
2 |
0.1361 |
Tier III |
Other clones with same target sequence:
(none)
