Construct: sgRNA BRDN0001147513
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAAGAACGAGATCAACCCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ETNK2 (55224)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76168
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
204146647 |
- |
ETNK2 |
NNNAGAACGAGATCAACCCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
2762240 |
- |
SRRM2 |
NNNAGAACGAGATCTACCCC |
NAG |
2 |
0.0519 |
Tier I |
| 3 |
NC_000013.11 |
13 |
23267379 |
+ |
SGCG |
NNNACAACGAAATCAACCCC |
NGG |
2 |
0.7857 |
Tier II |
| 4 |
NC_000009.12 |
9 |
126336234 |
+ |
MVB12B |
NNNAAAACGAGATAAACCCC |
NGG |
2 |
0.3033 |
Tier II |
| 5 |
NC_000013.11 |
13 |
23267379 |
+ |
SDAD1P4 |
NNNACAACGAAATCAACCCC |
NGG |
2 |
0.7857 |
Tier III |
| 6 |
NC_000002.12 |
2 |
193155718 |
- |
LOC107985969 |
NNNAGAAAGAGATCAACTCC |
NGG |
2 |
0.4179 |
Tier III |
| 7 |
NC_000012.12 |
12 |
119231665 |
- |
LOC105370025 |
NNNAGAACGAGATCAACGCC |
NTG |
2 |
0.0052 |
Tier III |
| 8 |
NC_000010.11 |
10 |
6122798 |
- |
LOC101928080 |
NNNAGAAAGAGATCATCCCC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
102001466 |
+ |
Numa1 |
NNNAGAACTAGATCAAGCCC |
NGG |
2 |
0.0314 |
Tier I |
| 2 |
NC_000068.7 |
2 |
33878727 |
- |
Mvb12b |
NNNAAAACGAGATAAACCCC |
NGG |
2 |
0.3033 |
Tier II |
| 3 |
NC_000076.6 |
10 |
21302953 |
- |
Hbs1l |
NNNAGAAAGAGATCAATCCC |
NGG |
2 |
0.3033 |
Tier II |
| 4 |
NC_000083.6 |
17 |
70879662 |
- |
Gm26510 |
NNNAGAAGGAGACCAACCCC |
NGG |
2 |
0.5075 |
Tier III |
| 5 |
NC_000068.7 |
2 |
153719374 |
- |
Dnmt3b-ps1 |
NNNAGAAAGAGATGAACCCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
