Construct: sgRNA BRDN0001147513

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAAGAACGAGATCAACCCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETNK2 (55224)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76168

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 204146647 - ETNK2 NNNAGAACGAGATCAACCCC NGG 0 1.0 Tier I
2 NC_000016.10 16 2762240 - SRRM2 NNNAGAACGAGATCTACCCC NAG 2 0.0519 Tier I
3 NC_000013.11 13 23267379 + SGCG NNNACAACGAAATCAACCCC NGG 2 0.7857 Tier II
4 NC_000009.12 9 126336234 + MVB12B NNNAAAACGAGATAAACCCC NGG 2 0.3033 Tier II
5 NC_000013.11 13 23267379 + SDAD1P4 NNNACAACGAAATCAACCCC NGG 2 0.7857 Tier III
6 NC_000002.12 2 193155718 - LOC107985969 NNNAGAAAGAGATCAACTCC NGG 2 0.4179 Tier III
7 NC_000012.12 12 119231665 - LOC105370025 NNNAGAACGAGATCAACGCC NTG 2 0.0052 Tier III
8 NC_000010.11 10 6122798 - LOC101928080 NNNAGAAAGAGATCATCCCC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 102001466 + Numa1 NNNAGAACTAGATCAAGCCC NGG 2 0.0314 Tier I
2 NC_000068.7 2 33878727 - Mvb12b NNNAAAACGAGATAAACCCC NGG 2 0.3033 Tier II
3 NC_000076.6 10 21302953 - Hbs1l NNNAGAAAGAGATCAATCCC NGG 2 0.3033 Tier II
4 NC_000083.6 17 70879662 - Gm26510 NNNAGAAGGAGACCAACCCC NGG 2 0.5075 Tier III
5 NC_000068.7 2 153719374 - Dnmt3b-ps1 NNNAGAAAGAGATGAACCCC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)