Construct: sgRNA BRDN0001147517
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGTCACTTCTTCGTATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP7 (143098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75833
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
28089735 |
+ |
MPP7 |
NNNGTCACTTCTTCGTATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
102523407 |
- |
MMP7 |
NNNGTCACTTCTTCGGTTGT |
NGG |
2 |
0.0462 |
Tier I |
| 3 |
NC_000008.11 |
8 |
40673270 |
- |
ZMAT4 |
NNNGTCACTTCTTAATATGT |
NGG |
2 |
0.3294 |
Tier II |
| 4 |
NC_000006.12 |
6 |
136445613 |
- |
MAP7 |
NNNGTCACTTCTACCTATGT |
NGG |
2 |
0.1888 |
Tier II |
| 5 |
NC_000006.12 |
6 |
27443405 |
+ |
ZNF184 |
NNNGTCACTTCTTCATTTGT |
NGG |
2 |
0.1255 |
Tier II |
| 6 |
NC_000004.12 |
4 |
173265312 |
- |
GALNT7 |
NNNGTCACTTCTTAGTATGT |
NAG |
2 |
0.0907 |
Tier II |
| 7 |
NC_000012.12 |
12 |
3406902 |
+ |
PRMT8 |
NNNGTCACTTCTTCGTTTTT |
NGG |
2 |
0.0889 |
Tier II |
| 8 |
NC_000012.12 |
12 |
129407346 |
+ |
TMEM132D |
NNNGTCACTTCTTCGTCTGT |
NAG |
2 |
0.0458 |
Tier II |
| 9 |
NC_000015.10 |
15 |
96301398 |
- |
NR2F2-AS1 |
NNNGTCACTTCTTTTTATGT |
NGG |
2 |
0.1048 |
Tier III |
| 10 |
NC_000004.12 |
4 |
173265312 |
- |
LOC124900812 |
NNNGTCACTTCTTAGTATGT |
NAG |
2 |
0.0907 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
101199841 |
+ |
Jak1 |
NNNGTCACTTCTTAGTAGGT |
NGG |
2 |
0.1167 |
Tier II |
| 2 |
NC_000077.6 |
11 |
114897514 |
+ |
Cd300a |
NNNGTCACTTCTTCCTCTGT |
NGG |
2 |
0.0481 |
Tier II |
| 3 |
NC_000068.7 |
2 |
79966599 |
+ |
Pde1a |
NNNGTCACTTCTTGGTGTGT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000067.6 |
1 |
130680204 |
- |
C4bp-ps1 |
NNNGTCACTTATTCATATGT |
NGG |
2 |
0.7059 |
Tier III |
| 5 |
NC_000072.6 |
6 |
41645733 |
- |
Gm34642 |
NNNGACACTTCTTTGTATGT |
NGG |
2 |
0.3667 |
Tier III |
| 6 |
NC_000072.6 |
6 |
58091991 |
- |
Gm6695 |
NNNTTCACTTCATCGTATGT |
NGG |
2 |
0.2909 |
Tier III |
Other clones with same target sequence:
(none)
