Construct: sgRNA BRDN0001147521
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAAGACGACTCCATCGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK4 (440275)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75877
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
39967447 |
+ |
EIF2AK4 |
NNNAGACGACTCCATCGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
45524569 |
- |
VASP |
NNNAGACGACTTCATCCTGG |
NGG |
2 |
0.1267 |
Tier I |
| 3 |
NC_000001.11 |
1 |
65551077 |
+ |
LEPR |
NNNAGCCGACTTCATCGTGG |
NGG |
2 |
0.2448 |
Tier II |
| 4 |
NC_000003.12 |
3 |
85225962 |
+ |
CADM2 |
NNNAGCCGACTTCATCGTGG |
NGG |
2 |
0.2448 |
Tier II |
| 5 |
NC_000004.12 |
4 |
20978674 |
- |
KCNIP4 |
NNNAGATGACTCCATGGTGG |
NGG |
2 |
0.125 |
Tier II |
| 6 |
NC_000022.11 |
22 |
28040390 |
- |
TTC28 |
NNNAGACGACTTGATCGTGG |
NGG |
2 |
0.0734 |
Tier II |
| 7 |
NC_000018.10 |
18 |
50750881 |
+ |
LOC105372116 |
NNNAGACGACTACATCATGG |
NGG |
2 |
0.6667 |
Tier III |
| 8 |
NC_000002.12 |
2 |
37596528 |
+ |
LINC03063 |
NNNAGCCGACTTCATCGTGG |
NGG |
2 |
0.2448 |
Tier III |
| 9 |
NC_000008.11 |
8 |
93154173 |
- |
LINC02906 |
NNNAGAAGACTCCATTGTGG |
NGG |
2 |
0.2308 |
Tier III |
| 10 |
NC_000001.11 |
1 |
218915128 |
+ |
LINC01710 |
NNNAGAAGACTCCATCTTGG |
NGG |
2 |
0.1875 |
Tier III |
| 11 |
NC_000023.11 |
X |
22870489 |
- |
PTCHD1-AS |
NNNAGCCGACTCGATCGTGG |
NGG |
2 |
0.062 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
39064161 |
+ |
Tusc3 |
NNNAGAGGACTCCATTGTGG |
NGG |
2 |
0.1448 |
Tier II |
| 2 |
NC_000069.6 |
3 |
136180923 |
+ |
Bank1 |
NNNAGACGAGTCCATCCTGG |
NGG |
2 |
0.0915 |
Tier II |
| 3 |
NC_000076.6 |
10 |
45660222 |
+ |
Hace1 |
NNNAGACGACTTGATCGTGG |
NGG |
2 |
0.0734 |
Tier II |
| 4 |
NC_000070.6 |
4 |
127914859 |
+ |
Gm40256 |
NNNAGACAACTCCATCGTGT |
NGG |
2 |
0.7 |
Tier III |
| 5 |
NC_000083.6 |
17 |
21848337 |
+ |
Gm31758 |
NNNAGACTACTTCATCGTGG |
NGG |
2 |
0.3365 |
Tier III |
Other clones with same target sequence:
(none)
