Construct: sgRNA BRDN0001147538
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGTGATGATATAGAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABL1 (25)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77735
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
130872883 |
- |
ABL1 |
NNNGTGATGATATAGAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
53425583 |
+ |
TSHZ2 |
NNNGTGATGATATAAAACAG |
NGG |
2 |
0.6723 |
Tier II |
| 3 |
NC_000005.10 |
5 |
69190557 |
- |
CENPH |
NNNGTTATGATATAGAACAG |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000012.12 |
12 |
129432038 |
- |
TMEM132D |
NNNGTGATGATATAGAATTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000022.11 |
22 |
24514818 |
- |
UPB1 |
NNNGTCATTATATAGAACGG |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000004.12 |
4 |
93619807 |
+ |
GRID2 |
NNNGTGATCATATAGAACTG |
NGG |
2 |
0.359 |
Tier II |
| 7 |
NC_000004.12 |
4 |
103086076 |
- |
BDH2 |
NNNGTGATGATATATAACAG |
NGG |
2 |
0.102 |
Tier II |
| 8 |
NC_000013.11 |
13 |
113537854 |
- |
TMCO3 |
NNNGTGATGCTATAGAAGGG |
NGG |
2 |
0.0444 |
Tier II |
| 9 |
NC_000002.12 |
2 |
158170022 |
- |
CCDC148-AS1 |
NNNGTGATAATATAGAACGA |
NGG |
2 |
0.6027 |
Tier III |
| 10 |
NC_000003.12 |
3 |
151784224 |
- |
AADACP1 |
NNNGTGATGATATAGGACAG |
NGG |
2 |
0.1374 |
Tier III |
| 11 |
NC_000003.12 |
3 |
151784224 |
- |
AADACL2-AS1 |
NNNGTGATGATATAGGACAG |
NGG |
2 |
0.1374 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
59280891 |
+ |
Slc18a2 |
NNNGTGATGATACAGAAGGG |
NGG |
2 |
0.1053 |
Tier II |
| 2 |
NC_000071.6 |
5 |
99474853 |
- |
A930011G23Rik |
NNNGTGATGATATAGAAATG |
NGG |
2 |
0.359 |
Tier III |
Other clones with same target sequence:
(none)
