Construct: sgRNA BRDN0001147542
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACTGATATCGAATGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MET (4233)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76062
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
116699718 |
- |
MET |
NNNACTGATATCGAATGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
48945071 |
- |
SHC4 |
NNNACTGTTATCGAAAGCAA |
NGG |
2 |
0.3896 |
Tier II |
| 3 |
NC_000012.12 |
12 |
91150079 |
+ |
DCN |
NNNATTGATATGGAATGCAA |
NGG |
2 |
0.2828 |
Tier II |
| 4 |
NC_000011.10 |
11 |
87964246 |
- |
RAB38 |
NNNACTGATCACGAATGCAA |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000002.12 |
2 |
135025841 |
- |
MAP3K19 |
NNNGCTGATATCTAATGCAA |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000015.10 |
15 |
80477503 |
- |
ARNT2 |
NNNACTGATCTTGAATGCAA |
NGG |
2 |
0.1795 |
Tier II |
| 7 |
NC_000013.11 |
13 |
41156083 |
- |
KBTBD6-DT |
NNNAATAATATCGAATGCAA |
NGG |
2 |
0.5714 |
Tier III |
| 8 |
NC_000003.12 |
3 |
28642816 |
- |
LINC00693 |
NNNACTGATATCAAAGGCAA |
NGG |
2 |
0.3195 |
Tier III |
| 9 |
NC_000021.9 |
21 |
25499739 |
+ |
LOC105372753 |
NNNACTGATATCAAATGCAA |
NTG |
2 |
0.036 |
Tier III |
| 10 |
NC_000013.11 |
13 |
91115779 |
- |
LOC105370312 |
NNNACTGATATCTAATGCAA |
NTG |
2 |
0.0117 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
115127356 |
+ |
Unc119b |
NNNACTGATATCGAACGAAA |
NGG |
2 |
0.359 |
Tier I |
| 2 |
NC_000086.7 |
X |
138561301 |
- |
Il1rapl2 |
NNNTCTGATATCAAATGCAA |
NGG |
2 |
0.5874 |
Tier II |
| 3 |
NC_000068.7 |
2 |
146456955 |
- |
Ralgapa2 |
NNNACTGATAGCGAATGCAA |
NAG |
2 |
0.1037 |
Tier II |
| 4 |
NC_000067.6 |
1 |
125724140 |
- |
Gpr39 |
NNNACTGATATCGAATGAAA |
NGT |
2 |
0.0087 |
Tier II |
| 5 |
NC_000071.6 |
5 |
25106579 |
- |
LOC115490109 |
NNNACTGATATAGTATGCAA |
NGG |
2 |
0.381 |
Tier III |
Other clones with same target sequence:
(none)
