Construct: sgRNA BRDN0001147547
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACATTCAACCAAACTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKG2 (5593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76919
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
81144316 |
- |
PRKG2 |
NNNACATTCAACCAAACTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
132693142 |
+ |
VNN1 |
NNNACATTCATCCAAGCTGT |
NGG |
2 |
0.0592 |
Tier I |
| 3 |
NC_000011.10 |
11 |
20497912 |
+ |
PRMT3 |
NNNACATTCAACCAAACAAT |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000002.12 |
2 |
28016314 |
+ |
BABAM2 |
NNNACATTCAACCAAACTCT |
NGG |
1 |
0.4483 |
Tier II |
| 5 |
NC_000003.12 |
3 |
158583638 |
+ |
MLF1 |
NNNATATTGAACCAAACTGT |
NGG |
2 |
0.3939 |
Tier II |
| 6 |
NC_000012.12 |
12 |
898001 |
+ |
WNK1 |
NNNACATTTAACCAAACTCT |
NGG |
2 |
0.3922 |
Tier II |
| 7 |
NC_000018.10 |
18 |
3708963 |
- |
DLGAP1 |
NNNGGATTCAACCAAACTGT |
NGG |
2 |
0.375 |
Tier II |
| 8 |
NC_000016.10 |
16 |
71006081 |
+ |
HYDIN |
NNNACAGTGAACCAAACTGT |
NGG |
2 |
0.3641 |
Tier II |
| 9 |
NC_000006.12 |
6 |
127766324 |
- |
THEMIS |
NNNTCATTCAACAAAACTGT |
NGG |
2 |
0.2448 |
Tier II |
| 10 |
NC_000003.12 |
3 |
41753438 |
+ |
ULK4 |
NNNACATTAAACCAAACTGT |
NAG |
2 |
0.2222 |
Tier II |
| 11 |
NC_000012.12 |
12 |
25094930 |
- |
IRAG2 |
NNNACATTCAACAAAATTGT |
NGG |
2 |
0.1795 |
Tier II |
| 12 |
NC_000008.11 |
8 |
26372387 |
- |
PPP2R2A |
NNNACATACAACCAAGCTGT |
NGG |
2 |
0.1538 |
Tier II |
| 13 |
NC_000007.14 |
7 |
18320146 |
+ |
HDAC9 |
NNNACATTCAACCACACTGA |
NGG |
2 |
0.1534 |
Tier II |
| 14 |
NC_000011.10 |
11 |
28035819 |
+ |
KIF18A |
NNNACATTCAACCATACTTT |
NGG |
2 |
0.1333 |
Tier II |
| 15 |
NC_000002.12 |
2 |
49106623 |
+ |
FSHR |
NNNAAATTCAACCAAACTGC |
NGG |
2 |
0.0519 |
Tier II |
| 16 |
NC_000002.12 |
2 |
162763595 |
+ |
KCNH7 |
NNNACATTCAACCAAATTGC |
NGG |
2 |
0.0424 |
Tier II |
| 17 |
NC_000007.14 |
7 |
123952850 |
+ |
SPAM1 |
NNNAAATTCAACCAAACTGT |
NGA |
2 |
0.0397 |
Tier II |
| 18 |
NC_000009.12 |
9 |
71402095 |
+ |
TRPM3 |
NNNACATTCAAACAAACTGT |
NGT |
2 |
0.0115 |
Tier II |
| 19 |
NC_000018.10 |
18 |
6043956 |
- |
L3MBTL4 |
NNNACAGTCAACCAAACTGT |
NGT |
2 |
0.0095 |
Tier II |
| 20 |
NC_000005.10 |
5 |
35730423 |
+ |
SPEF2 |
NNNACATTCAACCAACCTGT |
NGC |
2 |
0.0 |
Tier II |
| 21 |
NC_000005.10 |
5 |
23970706 |
+ |
LINC02899 |
NNNACATGGAACCAAACTGT |
NGG |
2 |
0.454 |
Tier III |
| 22 |
NC_000002.12 |
2 |
28016314 |
+ |
FAM133EP |
NNNACATTCAACCAAACTCT |
NGG |
1 |
0.4483 |
Tier III |
| 23 |
NC_000001.11 |
1 |
146705178 |
- |
HYDIN2 |
NNNACAGTGAACCAAACTGT |
NGG |
2 |
0.3641 |
Tier III |
| 24 |
NC_000002.12 |
2 |
40875940 |
- |
LOC105374497 |
NNNACATTTAACAAAACTGT |
NGG |
2 |
0.3365 |
Tier III |
| 25 |
NC_000005.10 |
5 |
152148901 |
+ |
LINC01933 |
NNNACATTCAACCAAACTGT |
NCT |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
98973192 |
- |
Prkg2 |
NNNACATTCAACCAAACTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
56565851 |
+ |
Cenpj |
NNNACACTCTACCAAACTGT |
NGG |
2 |
0.6066 |
Tier II |
| 3 |
NC_000080.6 |
14 |
11786591 |
- |
Ptprg |
NNNAAATGCAACCAAACTGT |
NGG |
2 |
0.419 |
Tier II |
| 4 |
NC_000067.6 |
1 |
150673449 |
- |
Hmcn1 |
NNNACAATCAACCAAACGGT |
NGG |
2 |
0.2917 |
Tier II |
| 5 |
NC_000070.6 |
4 |
44825133 |
- |
Zcchc7 |
NNNACATTCATACAAACTGT |
NGG |
2 |
0.2198 |
Tier II |
| 6 |
NC_000082.6 |
16 |
41294400 |
+ |
Lsamp |
NNNACATTCACCCAAATTGT |
NGG |
2 |
0.1867 |
Tier II |
| 7 |
NC_000076.6 |
10 |
32135714 |
- |
Nkain2 |
NNNACATTCATCCAAACTGA |
NGG |
2 |
0.1731 |
Tier II |
| 8 |
NC_000081.6 |
15 |
101427682 |
+ |
Krt7 |
NNNACATACAACCAAGCTGT |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000086.7 |
X |
111642628 |
- |
Hdx |
NNNACATTCATCCAAATTGT |
NGG |
2 |
0.1436 |
Tier II |
| 10 |
NC_000078.6 |
12 |
103675772 |
+ |
Serpina16 |
NNNATATTCAACCAAACTGT |
NGA |
2 |
0.0442 |
Tier II |
| 11 |
NC_000069.6 |
3 |
129922968 |
+ |
Mcub |
NNNAAATTCAACCAAAGTGT |
NGG |
2 |
0.0336 |
Tier II |
| 12 |
NC_000079.6 |
13 |
36048275 |
+ |
Lyrm4 |
NNNACACTCAACCAAACTGT |
NTG |
2 |
0.0268 |
Tier II |
| 13 |
NC_000076.6 |
10 |
71105902 |
+ |
Bicc1 |
NNNACCTTCAACCAAAGTGT |
NGG |
2 |
0.0267 |
Tier II |
| 14 |
NC_000070.6 |
4 |
118075945 |
- |
St3gal3 |
NNNACATTCAACCAAACTTT |
NTG |
2 |
0.026 |
Tier II |
| 15 |
NC_000069.6 |
3 |
131265905 |
+ |
Hadh |
NNNACATTCAACAAAAGTGT |
NGG |
2 |
0.0226 |
Tier II |
| 16 |
NC_000073.6 |
7 |
14489713 |
- |
Sult2a7 |
NNNACATTCAACCAGTCTGT |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000075.6 |
9 |
73255165 |
- |
Gm27211 |
NNNACATTCAACTAAACTTT |
NGG |
2 |
0.4667 |
Tier III |
| 18 |
NC_000077.6 |
11 |
40427730 |
+ |
Gm34173 |
NNNAAATTCAACAAAACTGT |
NGG |
2 |
0.2198 |
Tier III |
| 19 |
NC_000069.6 |
3 |
149597280 |
- |
Gm31121 |
NNNACACTCAACCAAACTGT |
NTG |
2 |
0.0268 |
Tier III |
Other clones with same target sequence:
(none)
