Construct: sgRNA BRDN0001147552
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGCTGTTAGAAGGCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STKLD1 (169436)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75811
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
133397277 |
+ |
STKLD1 |
NNNGCTGTTAGAAGGCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
17793509 |
+ |
C21orf91 |
NNNGCTGTTCGATGGCAACG |
NGG |
2 |
0.1 |
Tier I |
| 3 |
NC_000004.12 |
4 |
4440947 |
+ |
STX18 |
NNNGCAGTTAGAAGGCAACT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000001.11 |
1 |
121257418 |
- |
SRGAP2C |
NNNGCTATTAGAAGGCAATG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000001.11 |
1 |
145022881 |
+ |
SRGAP2B |
NNNGCTATTAGAAGGCAATG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000001.11 |
1 |
206275919 |
- |
SRGAP2 |
NNNGCTATTAGAAGGCAATG |
NGG |
2 |
0.4615 |
Tier II |
| 7 |
NC_000003.12 |
3 |
55599211 |
- |
ERC2 |
NNNGCTGTAAGCAGGCAACG |
NGG |
2 |
0.2444 |
Tier II |
| 8 |
NC_000006.12 |
6 |
163309693 |
- |
PACRG |
NNNGCTGTTAGAAGGCAACG |
NAC |
2 |
0.0 |
Tier II |
| 9 |
NC_000021.9 |
21 |
39029312 |
+ |
LINC02943 |
NNNGGTGTTAGAATGCAACG |
NGG |
2 |
0.16 |
Tier III |
| 10 |
NC_000021.9 |
21 |
17793509 |
+ |
LOC124900465 |
NNNGCTGTTCGATGGCAACG |
NGG |
2 |
0.1 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
91111423 |
- |
4932438H23Rik |
NNNGCTGTTAGAAGACAACA |
NGG |
2 |
0.8824 |
Tier II |
| 2 |
NC_000071.6 |
5 |
30938838 |
- |
Cgref1 |
NNNGCTGTTAGAAGACAACG |
NAG |
2 |
0.244 |
Tier II |
| 3 |
NC_000073.6 |
7 |
130945023 |
- |
Htra1 |
NNNGCTGTTAGGAGGTAACG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000075.6 |
9 |
29476361 |
+ |
Ntm |
NNNGCTGTTAGAAGGTAAAG |
NGG |
2 |
0.1319 |
Tier II |
Other clones with same target sequence:
(none)
