Construct: sgRNA BRDN0001147554

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACATCATCTTGATGGCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PINK1 (65018)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78035

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 20638102 - PINK1 NNNTCATCTTGATGGCCAAG NGG 0 1.0 Tier I
2 NC_000017.11 17 42023809 - NKIRAS2 NNNTCATCTTGCTGGCCAAG NAG 2 0.0682 Tier I
3 NC_000023.11 X 101356853 + BTK NNNTCATCTTGATGGCCACG NCG 2 0.0222 Tier I
4 NC_000020.11 20 36367777 - DLGAP4 NNNTCATCCTGATGGCAAAG NGG 2 0.4308 Tier II
5 NC_000001.11 1 229599692 + TAF5L NNNTCATCTTGATGGCCATT NGG 2 0.3769 Tier II
6 NC_000002.12 2 80585540 - CTNNA2 NNNTTATCTTCATGGCCAAG NGG 2 0.2727 Tier II
7 NC_000001.11 1 56551670 - PLPP3 NNNTCATCTTGATGGACAAG NAG 2 0.2593 Tier II
8 NC_000005.10 5 79324130 - JMY NNNTCATCTTGATGGACAAG NAG 2 0.2593 Tier II
9 NC_000003.12 3 125252577 - ZNF148 NNNTCATCATGCTGGCCAAG NGG 2 0.2444 Tier II
10 NC_000011.10 11 131567792 - NTM NNNTCATTTTGATGGGCAAG NGG 2 0.1346 Tier II
11 NC_000003.12 3 11022074 - SLC6A1 NNNTCATCTGGATGGCCCAG NGG 2 0.0952 Tier II
12 NC_000005.10 5 109132667 + FER NNNTCATGTTGATGTCCAAG NGG 2 0.0918 Tier II
13 NC_000013.11 13 20011959 + ZMYM2 NNNTCACCTTGATGGCCAAG NTG 2 0.0268 Tier II
14 NC_000015.10 15 80912500 + CEMIP NNNTCAGCTTGATGGCCAAG NTG 2 0.0229 Tier II
15 NC_000012.12 12 5771589 - ANO2 NNNTCATCTTGATGGCCAGG NTG 2 0.0146 Tier II
16 NC_000014.9 14 55589519 + KTN1 NNNTCATCTTGTTGGCCAAG NTG 2 0.013 Tier II
17 NC_000012.12 12 49316821 - TROAP-AS1 NNNTCACCATGATGGCCAAG NGG 2 0.6384 Tier III
18 NC_000011.10 11 81883076 + MIR4300HG NNNTCATCATGTTGGCCAAG NGG 2 0.3095 Tier III
19 NC_000005.10 5 79324130 - LOC102724530 NNNTCATCTTGATGGACAAG NAG 2 0.2593 Tier III
20 NC_000006.12 6 113874858 + LOC124901382 NNNTCATCTTGGTGGCCCAG NGG 2 0.1376 Tier III
21 NC_000007.14 7 125224480 - LOC101928283 NNNTCATCTTTATTGCCAAG NGG 2 0.1026 Tier III
22 NC_000019.10 19 29298201 - VSTM2B-DT NNNTCATCTTTATGGCCAAG NAG 2 0.0997 Tier III
23 NC_000007.14 7 65029049 + LOC105375329 NNNTCATCTTGTTGGCCAAG NTG 2 0.013 Tier III
24 NC_000007.14 7 65029049 + LOC105375330 NNNTCATCTTGTTGGCCAAG NTG 2 0.013 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 138320766 + Pink1 NNNTCATCTTGATGGCAAAG NGA 2 0.0324 Tier I
2 NC_000077.6 11 3977954 - Pes1 NNNTCATCATGATGGCCAAG NGC 2 0.0206 Tier I
3 NC_000080.6 14 8369217 + Oit1 NNNTCATCTTGGTGGCCAAG NGG 1 0.7222 Tier II
4 NC_000075.6 9 119629104 + Scn10a NNNTCTTCCTGATGGCCAAG NGG 2 0.6593 Tier II
5 NC_000071.6 5 115286879 - Coq5 NNNTCATCATGAAGGCCAAG NGG 2 0.6429 Tier II
6 NC_000082.6 16 35573468 + Sema5b NNNTCATCTGGATGGACAAG NGG 2 0.5 Tier II
7 NC_000067.6 1 105709395 + Relch NNNTAATCTTGATAGCCAAG NGG 2 0.4286 Tier II
8 NC_000085.6 19 56437531 + Casp7 NNNTCATCTTGGTGGCCTAG NGG 2 0.3611 Tier II
9 NC_000077.6 11 58850961 + Gm12258 NNNTCATCTTGATTACCAAG NGG 2 0.251 Tier II
10 NC_000080.6 14 67405269 - Ebf2 NNNTAATCTTGATGGCCAAC NGG 2 0.2449 Tier II
11 NC_000071.6 5 141431845 - Sdk1 NNNTCCTCTTGATGGCTAAG NGG 2 0.2121 Tier II
12 NC_000073.6 7 79780023 + Wdr93 NNNACATCTTGATCGCCAAG NGG 2 0.2041 Tier II
13 NC_000075.6 9 31177487 + Aplp2 NNNTCATCTTGATGGTCATG NGG 2 0.1657 Tier II
14 NC_000076.6 10 73114438 + Pcdh15 NNNTTATCTTGATGGCCAAG NAG 2 0.165 Tier II
15 NC_000068.7 2 62618870 + Ifih1 NNNTCATCTTCTTGGCCAAG NGG 2 0.1429 Tier II
16 NC_000068.7 2 27126877 + Fam163b NNNTCAACTTGATGGGCAAG NGG 2 0.1346 Tier II
17 NC_000083.6 17 43897699 - Rcan2 NNNTCATCTTGTTGGCCAGG NGG 2 0.125 Tier II
18 NC_000077.6 11 68444133 - Pik3r5 NNNTCCTCTTGCTGGCCAAG NGG 2 0.1196 Tier II
19 NC_000069.6 3 122054542 + Abca4 NNNTCATCTTTATGCCCAAG NGG 2 0.1049 Tier II
20 NC_000072.6 6 34933924 - Stra8 NNNTCATCTTGAGGGCCACG NGG 2 0.054 Tier II
21 NC_000071.6 5 26860284 - Dpp6 NNNACATCTTGATGGCCAAG NGA 2 0.0331 Tier II
22 NC_000074.6 8 99326639 - Cdh8 NNNTCATCTTGATCGCCAAG NTG 2 0.0167 Tier II
23 NC_000069.6 3 110025798 + Ntng1 NNNTCATCTTGATGGCCAAA NGT 2 0.0151 Tier II
24 NC_000070.6 4 62018994 - Mup20 NNNTCATCTTTATGGCCAAG NTG 2 0.015 Tier II
25 NC_000079.6 13 17653891 + Sugct NNNTCATCTTTATGGCCAAG NTG 2 0.015 Tier II
26 NC_000080.6 14 8369217 + 2610318M16Rik NNNTCATCTTGGTGGCCAAG NGG 1 0.7222 Tier III
27 NC_000082.6 16 35573468 + Gm5963 NNNTCATCTGGATGGACAAG NGG 2 0.5 Tier III
28 NC_000075.6 9 31177487 + Gm33535 NNNTCATCTTGATGGTCATG NGG 2 0.1657 Tier III
29 NC_000074.6 8 83477634 + Gm5910 NNNTCATCATGATGGCCAAG NGC 2 0.0206 Tier III
30 NC_000081.6 15 45978521 + Gm5472 NNNTCATCATGATGGCCAAG NGC 2 0.0206 Tier III
31 NC_000077.6 11 56092036 + LOC115485826 NNNTCATCTTTATGGCCAAG NTG 2 0.015 Tier III
32 NC_000074.6 8 86814079 + Gm39215 NNNTCATCTTGATTGCCAAG NTG 2 0.0104 Tier III
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Other clones with same target sequence:

(none)