Construct: sgRNA BRDN0001147557

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAGTAATGAGCATCCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADK (132)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75745

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 74394241 - ADK NNNGTAATGAGCATCCACAT NGG 0 1.0 Tier I
2 NC_000007.14 7 87744796 - RUNDC3B NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
3 NC_000012.12 12 11043480 + PRH1 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
4 NC_000012.12 12 11043480 + PRH1-TAS2R14 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
5 NC_000012.12 12 11117103 + PRH1 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
6 NC_000012.12 12 11117103 + PRH1-TAS2R14 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
7 NC_000012.12 12 79279691 + SYT1 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier II
8 NC_000007.14 7 133878672 - EXOC4 NNNGAAATGAACATCCACAT NGG 2 0.5 Tier II
9 NC_000005.10 5 133514406 + FSTL4 NNNATAATTAGCATCCACAT NGG 2 0.48 Tier II
10 NC_000023.11 X 64930346 - ZC4H2 NNNGTAAAGAGCATCCAAAT NGG 2 0.4308 Tier II
11 NC_000023.11 X 36119049 - CFAP47 NNNGTAATAAGCATCCAAAT NGG 2 0.3462 Tier II
12 NC_000001.11 1 37628909 - RSPO1 NNNGTAATGAGCATCCACAT NAG 1 0.2593 Tier II
13 NC_000015.10 15 71881339 - MYO9A NNNGTAACGAGCATACACAT NGG 2 0.2222 Tier II
14 NC_000019.10 19 3635037 - PIP5K1C NNNGTACTGAGCACCCACAT NGG 2 0.125 Tier II
15 NC_000007.14 7 14739073 - DGKB NNNTTAATGAGCATCGACAT NGG 2 0.0559 Tier II
16 NC_000012.12 12 21497658 - RECQL NNNTTAATGAGCATTCACAT NGG 2 0.0242 Tier II
17 NC_000006.12 6 1688965 - GMDS NNNGTAATGATCATCCACAT NTG 2 0.015 Tier II
18 NC_000003.12 3 167818960 - SERPINI1 NNNGTAATGAGTATCCACAT NGT 2 0.0087 Tier II
19 NC_000012.12 12 11043480 + PRH1-PRR4 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier III
20 NC_000012.12 12 11117103 + PRH1-PRR4 NNNATAAAGAGCATCCACAT NGG 2 0.72 Tier III
21 NC_000003.12 3 83397764 + LOC105377181 NNNATAATGAGCATCCAAAT NGG 2 0.4846 Tier III
22 NC_000012.12 12 127292518 - LOC124903050 NNNTTAATGAGCAGCCACAT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 21234933 - Adk NNNGTAATGAGCATCCACAT NTG 1 0.039 Tier I
2 NC_000074.6 8 56010178 - Glra3 NNNGTTGTGAGCATCCACAT NGG 2 0.5042 Tier II
3 NC_000068.7 2 160690047 - Top1 NNNGTTATCAGCATCCACAT NGG 2 0.3846 Tier II
4 NC_000080.6 14 38899213 - Nrg3 NNNGTGTTGAGCATCCACAT NGG 2 0.3125 Tier II
5 NC_000084.6 18 75224674 - Dym NNNGTTCTGAGCATCCACAT NGG 2 0.3125 Tier II
6 NC_000071.6 5 102879384 - Arhgap24 NNNGTTTTGAGCATCCACAT NGG 2 0.3125 Tier II
7 NC_000075.6 9 32099843 - Arhgap32 NNNGTTTTGAGCATCCACAT NGG 2 0.3125 Tier II
8 NC_000072.6 6 91945932 + 4930590J08Rik NNNGTAATGAGCATCCACCT NGG 1 0.2069 Tier II
9 NC_000079.6 13 93712755 - Dmgdh NNNGTTATGAGCCTCCACAT NGG 2 0.1504 Tier II
10 NC_000076.6 10 90958720 + Anks1b NNNGTAGTGAGCCTCCACAT NGG 2 0.1486 Tier II
11 NC_000067.6 1 75203600 + Glb1l NNNGTAATGAGCCACCACAT NGG 2 0.1303 Tier II
12 NC_000073.6 7 57615671 - Gabrb3 NNNGAAATGAGCCTCCACAT NGG 2 0.1053 Tier II
13 NC_000076.6 10 67199158 + Jmjd1c NNNGTAGTGAGCATTCACAT NGG 2 0.0471 Tier II
14 NC_000082.6 16 17389282 - Pi4ka NNNGTAATGAGAATCCACAT NTG 2 0.0278 Tier II
15 NC_000084.6 18 60558656 + Dctn4 NNNGTAATGAGCATCCTCCT NGG 2 0.0276 Tier II
16 NC_000068.7 2 58864154 - Ccdc148 NNNGTAGTGAGCATCCACAT NTG 2 0.0275 Tier II
17 NC_000072.6 6 112804970 - Srgap3 NNNGTAATGAGCATCCACTT NTG 2 0.021 Tier II
18 NC_000078.6 12 102261413 + Slc24a4 NNNGTAATGAGTATCCACAT NGC 2 0.012 Tier II
19 NC_000081.6 15 77157526 + Rbfox2 NNNGTAATGAGCTTCCACAT NTG 2 0.0117 Tier II
20 NC_000085.6 19 44497598 + Wnt8b NNNGTAATGAGCATTCACAT NGA 2 0.0046 Tier II
21 NC_000069.6 3 37496909 - Spata5 NNNGTAATGAGCATCCACAG NGC 2 0.0039 Tier II
22 NC_000073.6 7 16832368 - Strn4 NNNGTAATGAGAAGCCACAT NGG 2 0.0 Tier II
23 NC_000070.6 4 40887738 + Gm34289 NNNGTTATGAGCAACCACAT NGG 2 0.4422 Tier III
24 NC_000080.6 14 38899213 - Nrg3os NNNGTGTTGAGCATCCACAT NGG 2 0.3125 Tier III
25 NC_000079.6 13 93712755 - AW495222 NNNGTTATGAGCCTCCACAT NGG 2 0.1504 Tier III
26 NC_000084.6 18 83222019 - Gm31089 NNNGTTATGAGCATCCACCT NGG 2 0.1478 Tier III
27 NC_000084.6 18 60558656 + Gm52358 NNNGTAATGAGCATCCTCCT NGG 2 0.0276 Tier III
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Other clones with same target sequence:

(none)