Construct: sgRNA BRDN0001147558
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAGCTCGTGAGTGCAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRK (203447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76314
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
105908737 |
- |
NRK |
NNNAGCTCGTGAGTGCAAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_012920.1 |
MT |
8064 |
- |
COX2 |
NNNAGCTCATGAGTGCAAGA |
NGT |
2 |
0.0104 |
Tier I |
| 3 |
NC_000019.10 |
19 |
48127382 |
+ |
LIG1 |
NNNAGTTCGTGAGTGCAGGA |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000011.10 |
11 |
15148398 |
+ |
INSC |
NNNTGCTCGTGATTGCAAGA |
NGG |
2 |
0.1909 |
Tier II |
| 5 |
NC_000016.10 |
16 |
89859488 |
+ |
SPIRE2 |
NNNAGCTCGTGCCTGCAAGA |
NGG |
2 |
0.1108 |
Tier II |
| 6 |
NC_000009.12 |
9 |
126405321 |
+ |
MVB12B |
NNNAGCTCGCGAGTGCACGA |
NGG |
2 |
0.1016 |
Tier II |
| 7 |
NC_000020.11 |
20 |
35523909 |
+ |
C20orf173 |
NNNAGCCCGTGAGTTCAAGA |
NGG |
2 |
0.0982 |
Tier II |
| 8 |
NC_000011.10 |
11 |
97399702 |
- |
LOC105369451 |
NNNAGCTTGAGAGTGCAAGA |
NGG |
2 |
0.75 |
Tier III |
| 9 |
NC_000003.12 |
3 |
8315791 |
+ |
LMCD1-AS1 |
NNNAGCTCCTGAGTGCCAGA |
NGG |
2 |
0.095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
138975147 |
- |
Nrk |
NNNAGCTCAGGAGTGCAAGA |
NGG |
2 |
0.3214 |
Tier I |
| 2 |
NC_000084.6 |
18 |
61721155 |
- |
Grpel2 |
NNNAGCACCTGAGTGCAAGA |
NGG |
2 |
0.4712 |
Tier II |
| 3 |
NC_000071.6 |
5 |
24922266 |
+ |
Prkag2 |
NNNAGCTCATGAGTGCCAGA |
NGG |
2 |
0.1134 |
Tier II |
| 4 |
NC_000074.6 |
8 |
114320107 |
- |
Vat1l |
NNNAGCTCGGGAGTTCAAGA |
NGG |
2 |
0.0714 |
Tier II |
| 5 |
NC_000073.6 |
7 |
130632770 |
- |
Tacc2 |
NNNAGCCCGTGAGTGCAAGA |
NGA |
2 |
0.0477 |
Tier II |
| 6 |
NC_000077.6 |
11 |
107714385 |
+ |
Cacng1 |
NNNAGCTGGTGAGGGCAAGA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000071.6 |
5 |
24922266 |
+ |
Gm52767 |
NNNAGCTCATGAGTGCCAGA |
NGG |
2 |
0.1134 |
Tier III |
| 8 |
NC_000079.6 |
13 |
71574299 |
+ |
1700112M02Rik |
NNNTGCTCGTGAGTGCAAGA |
NGC |
2 |
0.0141 |
Tier III |
Other clones with same target sequence:
(none)
