Construct: sgRNA BRDN0001147559
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTGTTTCACCTGACGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS2 (5634)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78005
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
12810131 |
+ |
PRPS2 |
NNNTGTTTCACCTGACGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
56351414 |
- |
STAT2 |
NNNTGTTTCACCTGAGGCAG |
NTG |
2 |
0.006 |
Tier I |
| 3 |
NC_000016.10 |
16 |
70704151 |
- |
VAC14 |
NNNGGTTTCACCTGAAGCAG |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000012.12 |
12 |
5660910 |
- |
ANO2 |
NNNTGTGTCACCTGAAGCAG |
NGG |
2 |
0.5882 |
Tier II |
| 5 |
NC_000021.9 |
21 |
44441552 |
+ |
TRPM2 |
NNNTGGTGCACCTGACGCAG |
NGG |
2 |
0.419 |
Tier II |
| 6 |
NC_000001.11 |
1 |
44243789 |
+ |
ERI3 |
NNNTGTTTCACTTGACGCTG |
NGG |
2 |
0.2899 |
Tier II |
| 7 |
NC_000015.10 |
15 |
101338010 |
- |
PCSK6 |
NNNTGTATCACCTGACCCAG |
NGG |
2 |
0.2059 |
Tier II |
| 8 |
NC_000008.11 |
8 |
69749346 |
+ |
SLCO5A1 |
NNNTGTTTCACCGGGCGCAG |
NGG |
2 |
0.1696 |
Tier II |
| 9 |
NC_000004.12 |
4 |
150165749 |
+ |
DCLK2 |
NNNTGTTTCACCTGATGCTG |
NGG |
2 |
0.1657 |
Tier II |
| 10 |
NC_000018.10 |
18 |
46520418 |
- |
LOXHD1 |
NNNTGTTTCACCTGACCCTG |
NGG |
2 |
0.1267 |
Tier II |
| 11 |
NC_000004.12 |
4 |
42919697 |
- |
GRXCR1 |
NNNTGTTTCCCCTGACCCAG |
NGG |
2 |
0.0784 |
Tier II |
| 12 |
NC_000001.11 |
1 |
230376298 |
+ |
PGBD5 |
NNNTGTTTCACCTGTCTCAG |
NGG |
2 |
0.05 |
Tier II |
| 13 |
NC_000002.12 |
2 |
99554772 |
- |
AFF3 |
NNNTGTTTCACCTCACGCAG |
NTG |
2 |
0.0167 |
Tier II |
| 14 |
NC_000008.11 |
8 |
117428545 |
+ |
LOC105375716 |
NNNTGTTTCACATGACACAG |
NGG |
2 |
0.6667 |
Tier III |
| 15 |
NC_000001.11 |
1 |
44243789 |
+ |
ERI3-IT1 |
NNNTGTTTCACTTGACGCTG |
NGG |
2 |
0.2899 |
Tier III |
| 16 |
NC_000017.11 |
17 |
30655597 |
- |
LOC105371723 |
NNNTGTTTCACCGGGCGCAG |
NGG |
2 |
0.1696 |
Tier III |
| 17 |
NC_000015.10 |
15 |
61627479 |
+ |
LOC107984782 |
NNNTGTTTCACCTGACGCCC |
NGG |
2 |
0.0887 |
Tier III |
| 18 |
NC_000010.11 |
10 |
28441282 |
- |
LINC02652 |
NNNTTTTTCACCTGAGGCAG |
NGG |
2 |
0.0462 |
Tier III |
| 19 |
NC_000010.11 |
10 |
28441282 |
- |
LOC107984216 |
NNNTTTTTCACCTGAGGCAG |
NGG |
2 |
0.0462 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
12416164 |
- |
Mindy3 |
NNNTGTTTCACCTGAAGCTG |
NGG |
2 |
0.5385 |
Tier II |
| 2 |
NC_000075.6 |
9 |
16443517 |
- |
Fat3 |
NNNTGTTTCACCTGACACGG |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000079.6 |
13 |
94265825 |
+ |
Scamp1 |
NNNTGTTTCACTTGATGCAG |
NGG |
2 |
0.1657 |
Tier II |
| 4 |
NC_000075.6 |
9 |
86851873 |
- |
Snap91 |
NNNTGTATCACCTGAGGCAG |
NGG |
2 |
0.1346 |
Tier II |
| 5 |
NC_000071.6 |
5 |
123587908 |
+ |
Clip1 |
NNNTGTGTCACCTGACGCCG |
NGG |
2 |
0.1217 |
Tier II |
| 6 |
NC_000076.6 |
10 |
4977375 |
- |
Esr1 |
NNNTGTTTCACCTGACACAG |
NGA |
2 |
0.0648 |
Tier II |
| 7 |
NC_000070.6 |
4 |
154918904 |
- |
Tnfrsf14 |
NNNTGTTTCACCTGCCCCAG |
NGG |
2 |
0.0642 |
Tier II |
| 8 |
NC_000067.6 |
1 |
133045824 |
+ |
Pik3c2b |
NNNTGTTTCACCTGACCCAG |
NGC |
2 |
0.0052 |
Tier II |
| 9 |
NC_000077.6 |
11 |
67760890 |
- |
Glp2r |
NNNTGTTTCACCTGACCCAG |
NGT |
2 |
0.0038 |
Tier II |
| 10 |
NC_000069.6 |
3 |
128145429 |
+ |
D030025E07Rik |
NNNGGTTTCTCCTGACGCAG |
NGG |
2 |
0.5515 |
Tier III |
Other clones with same target sequence:
(none)
