Construct: sgRNA BRDN0001147563
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGCACCTGTGAAATCGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK40 (83931)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75625
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
36355415 |
- |
STK40 |
NNNCACCTGTGAAATCGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
80443397 |
- |
TENT5D |
NNNCACCTATGAAATGGTTG |
NGG |
2 |
0.0989 |
Tier I |
| 3 |
NC_000023.11 |
X |
153774796 |
- |
PLXNB3 |
NNNCACCTGTGAACTCCTTG |
NGG |
2 |
0.0504 |
Tier I |
| 4 |
NC_000010.11 |
10 |
17788821 |
- |
TMEM236 |
NNNCAGCTGTGAAATAGTTG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000013.11 |
13 |
110913875 |
- |
ANKRD10 |
NNNCATCTGTGAAGTCGTTG |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000002.12 |
2 |
25547782 |
+ |
DTNB |
NNNCACCTGTGAAATAGTCG |
NGG |
2 |
0.2759 |
Tier II |
| 7 |
NC_000016.10 |
16 |
12360510 |
- |
SNX29 |
NNNCACCTGTGACATCGTTG |
NGG |
1 |
0.2105 |
Tier II |
| 8 |
NC_000001.11 |
1 |
7766307 |
- |
CAMTA1 |
NNNCTCCTGTGAAATTGTTG |
NGG |
2 |
0.1119 |
Tier II |
| 9 |
NC_000015.10 |
15 |
31101066 |
- |
TRPM1 |
NNNCACCTGTGAAAGCCTTG |
NGG |
2 |
0.0118 |
Tier II |
| 10 |
NC_000013.11 |
13 |
96973216 |
+ |
LINC00359 |
NNNCATCTGTGAAATCTTTG |
NGG |
2 |
0.2321 |
Tier III |
| 11 |
NC_000002.12 |
2 |
116295220 |
- |
LOC105373576 |
NNNCACCTGTGAAATCTTTG |
NAG |
2 |
0.0648 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
73767871 |
- |
Plxnb3 |
NNNCACCTGTGAACTCCTTG |
NGG |
2 |
0.0504 |
Tier I |
| 2 |
NC_000070.6 |
4 |
151062992 |
+ |
Camta1 |
NNNCACTTGTGAAATTGTTG |
NGG |
2 |
0.25 |
Tier II |
| 3 |
NC_000075.6 |
9 |
71878046 |
+ |
Tcf12 |
NNNCAACTGTGAAAGCGTTG |
NGG |
2 |
0.0464 |
Tier II |
| 4 |
NC_000079.6 |
13 |
10407174 |
+ |
Gm26861 |
NNNCACTTGTGAAATCCTTG |
NGG |
2 |
0.1912 |
Tier III |
Other clones with same target sequence:
(none)
