Construct: sgRNA BRDN0001147564
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCCGGACAGAGTTTGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HK1 (3098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77644
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
69369582 |
+ |
HK1 |
NNNCCGGACAGAGTTTGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
77253336 |
+ |
TMEM63C |
NNNCCAGACAGTGTTTGACA |
NGG |
2 |
0.3333 |
Tier I |
| 3 |
NC_000002.12 |
2 |
100093758 |
- |
AFF3 |
NNNCAGGACAGAGTTTGACT |
NGG |
2 |
0.3429 |
Tier II |
| 4 |
NC_000002.12 |
2 |
109771427 |
+ |
RANBP2 |
NNNCAGGACAGAGTTTGACT |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000002.12 |
2 |
109771427 |
+ |
RGPD5 |
NNNCAGGACAGAGTTTGACT |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000002.12 |
2 |
110600060 |
- |
RGPD6 |
NNNCAGGACAGAGTTTGACT |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000008.11 |
8 |
3672649 |
+ |
CSMD1 |
NNNCCGGTCAGAGTATGACA |
NGG |
2 |
0.2481 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6509726 |
+ |
Nrxn2 |
NNNGCAGACAGAGTTTGACA |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000078.6 |
12 |
98935815 |
- |
Ttc8 |
NNNCCAGACAGTGTTTGACA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000085.6 |
19 |
6854343 |
- |
Ccdc88b |
NNNGCGGACAGAGTTTGACT |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000069.6 |
3 |
97695426 |
+ |
Pde4dip |
NNNCCTGACAGAGTTTGGCA |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000071.6 |
5 |
16794105 |
+ |
Gm28710 |
NNNCAGGTCAGAGTTTGACA |
NGG |
2 |
0.2449 |
Tier II |
| 6 |
NC_000075.6 |
9 |
22023299 |
- |
Elavl3 |
NNNCCTGACAGAGCTTGACA |
NGG |
2 |
0.1905 |
Tier II |
| 7 |
NC_000086.7 |
X |
113804877 |
+ |
Dach2 |
NNNCCAGACAGAGTTTGAGA |
NGG |
2 |
0.125 |
Tier II |
Other clones with same target sequence:
(none)
